Computational identification of Chinese cabbage anthocyaninspecific genes

Abstract

Therefore, 3,895 candidate genes related to anthocyanin biosynthesis in Chinese cabbage were identified using a 300K Brassica rapa microarray analysis. Gene expression during six stages of leaf developmental stages were examined in FC (green leaf) and FA (red leaf) Chinese cabbage cultivars. The 317 transcription factor genes found to be associated with anthocyanin were classified into 11 functional groups. The ratio of expression levels of each transcription factor between the two cultivars was examined during the six leaf developmental stages. A total of 14 genes were found to be expressed in all developmental stages commonly. Among these genes, 10 unknown and hypothetical genes were differentially revealed to be expressed between the two cultivars at each developmental stage, as determined by microarray analysis, and were verified by RT-PCR validation. These genes most likely play regulatory roles in either anthocyanin production or metabolism during flavonoid biosynthesis. While these genes require further validation and characterization, our results illustrate the potential usefulness of this multi-layered screening method using Chinese cabbage (Brassica rapa) microarrays.

Double Gate Tunnel-FET Working Like a Permittivity Based Biosensor with Different Drain to Gate and Drain to Biomaterial Alignments

“…32 Therefore, the Bio-TFET consist to use this underlap region of length L UD as a sensitive area for biomolecules detection. 33 Biomolecules have different values of dielectric constant, e.g., DNA (k = 1-64), 34,35 streptavidin (k = 2.1), 36 biotin (k = 2.63), 36 APTES (k = 3.57) 37 and Iris antigen (k = 4). 38 Depositing a biomaterial that acts as a bioreceptor it is possible to bind target biomolecules in the L UD region.…”

Performance Analysis of MOS-HEMT as a Biosensor: A Dielectric Modulation Approach

“…The neutral biomolecules given in Table 1 are analyzed. The charged biomolecules are analyzed range from 3 × 10 12 /cm 2 to −5 × 10 12 /cm 2 , which includes the DNA charge ranges from −1 × 10 12 /cm 2 to −1 × 10 11 /cm 2 [9]. The dielectric of air(K=1) is considered as reference to differentiate the change in device performance parameters after exposure to different biomolecules.…”

“…where ✏ oxide oxide is the dielectric constant of oxide,✏ AlGaN is the dielectric constant and d AlGaN is width of the barrier layer, respectively, q b is the gate barrier height. The threshold voltage given in the below equation for a MOS-HEMT shows the dependence on the dielectric constant and thickness of oxide and barrier layer [9].…”

“…The presence of high sheet charge density and mobility increases the sensitivity [3]. Various research has been done on HEMT based biosensor for pH detection [4] [5][6] [7], Immuno-detection using floating gate AlGaN HEMT [8],deoxyribonucleic acid (DNA) [9], Urea testing [10], Prostate-specific antigen testing [11] [12], Monokine induced by interferon gamma [13], CerbB2 detection [14], CO and H 2 detection [3] [15], C-reactive protein detection [16], troponin I testing [17] [18] and carcinoembryonic antigen (CEA) detection respectively. To enhance the sensitivity of the proposed HEMTs device, various structures are also optimized like Circular gate HEMT, Double gate, Gateless devices, Nano-cavity under the gate region towards the drain side, and Nano-cavity at the source and drain both sides, respectively.…”

A genome-based database for agricultural biotechnology

“…The NABIC was established in 2002 with the purpose of analyzing the genomes of agricultural crops and providing related services to professional genomic research institutes and societies (NAAS, 2010;Kim et al, 2011b). Our genome-based database provides information through a user-friendly web interface from searching genetic resources to genome infrastructure analysis.…”