Computational investigation on lipid bilayer disruption induced by amphiphilic Janus nanoparticles: combined effect of Janus balance and charged lipid concentration

Nguyen, Danh; Wu, James; Corrigan, Patrick; Li, Ying

doi:10.1039/d3nr00403a

Cited by 5 publications

(1 citation statement)

References 65 publications

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“…Second, characterization of Janus structures at the <50 nm regime that occurs due to anisotropic surface chemistry and anisotropic functionalization is challenging due to the requirements of sophisticated chemical characterization at such length scales. Third, cellular interaction with Janus nanostructures is not well understood, and recent studies indicate that they can lead to interesting phenomena. − In particular, it has been shown that amphiphilic Janus nanoparticles can lead to cell membrane disruption, , anisotropically functionalized Janus nanoparticles offer extended cell surface attachment, and amphiphilic–polycationic Janus nanoparticles offer enhanced antibacterial activity . These results suggest that Janus nanoparticles with anisotropic functionalization have a wide range of application potentials that are yet to be explored.…”

Section: Introductionmentioning

confidence: 99%

Nanoporous Silica Nanoparticles with Janus Functionalization for Enhanced Cell Uptake and Drug Delivery

Garai,

Ghosh,

Jana

2024

ACS Appl. Nano Mater.

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Although colloidal Janus nanoparticles have significant biomedical application potential, their interaction with cells has been relatively less studied. Here we have demonstrated enhanced cellular interaction and uptake of nanoparticles due to Janus functionalization. In particular, we have synthesized 50−100 nm porous silica particles where one side of each particle is terminated with arginine and the other side is terminated with dextran. These Janus nanoparticles exhibit lower colloidal stability compared to isotropically functionalized arginine/dextran, and this property induces enhanced cellular interaction/uptake. The porous structure of these Janus nanoparticles is used for the loading of small-molecule drugs and then for enhanced cell delivery of drugs with enhanced therapeutic performance. Results suggest that Janus nanoparticles with anisotropic functionalization have a wide range of biomedical application potentials that are yet to be explored.

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Section: Introductionmentioning

confidence: 99%

Nanoporous Silica Nanoparticles with Janus Functionalization for Enhanced Cell Uptake and Drug Delivery

Garai,

Ghosh,

Jana

2024

ACS Appl. Nano Mater.

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Targeting the Weak Spot: Preferential Disruption of Bacterial Poles by Janus Nanoparticles

Nguyen,

Bhattacharyya,

Richman

et al. 2024

Nano Lett.

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The interaction between nanoparticles (NPs) and bacterial cell envelopes is crucial for designing effective antibacterial materials against multi-drug-resistant pathogens. However, the current understanding assumes a uniform bacterial cell wall. This study challenges that assumption by investigating how bacterial cell wall curvature impacts antibacterial NP action. Focusing on Janus NPs, which feature segregated hydrophobic and polycationic ligands and previously demonstrated high efficacy against diverse bacteria, we found that these NPs preferentially target and disrupt bacterial poles. Experimental and computational approaches reveal that curvature at E. coli poles induces conformational changes in lipopolysaccharide (LPS) polymers on the outer membrane, exposing underlying lipids for NPmediated disruption. We establish that curvature-induced targeting by Janus NPs depends on the outer membrane composition and is most pronounced at physiologically relevant LPS densities. This work demonstrates that high-curvature regions of bacterial cell walls are "weak spots" for Janus NPs, thereby aiding the development of more effective targeted therapies.

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Influence of branched ligand architectures on nanoparticle interactions with lipid bilayers

Huang-Zhu,

Van Lehn

2025

Nanoscale

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Computational investigation on lipid bilayer disruption induced by amphiphilic Janus nanoparticles: combined effect of Janus balance and charged lipid concentration

Abstract: Janus nanoparticles (NPs) with charged/hydrophobic compartments have garnered attention for their potential antimicrobial activity. These NPs have been shown to disrupt lipid bilayers in experimental studies, yet the underlying mechanisms...

Cited by 5 publications

References 65 publications

Nanoporous Silica Nanoparticles with Janus Functionalization for Enhanced Cell Uptake and Drug Delivery

Nanoporous Silica Nanoparticles with Janus Functionalization for Enhanced Cell Uptake and Drug Delivery

Targeting the Weak Spot: Preferential Disruption of Bacterial Poles by Janus Nanoparticles

Influence of branched ligand architectures on nanoparticle interactions with lipid bilayers

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