Computational Modeling of the Electrochemical System of Lipase Activity Detection

Abstract

This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5′-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process.

Phospholipase A2 activity on supported thiolipid monolayers monitored by electrochemical and SPR methods

“…The mechanism of phospholipase activation and use of these enzymes in liposome-based drug delivery have been extensively studied by the group of Jorgensen et al [5][6][7][8]. An electrochemical method for the detection of lipolytic enzyme activity based on its interaction with solid supported lipid substrate was described by Razumas and coworkers [9,10]. The interfacial enzymatic process liberated ferrocene groups from the electrode surface triggering a decay of the electrochemical signal.…”

Application of room temperature ionic liquids to the development of electrochemical lipase biosensing systems for water-insoluble analytes

“…It also could be caused by lipase kinetics, but usually its activity decreases in time [49]; thus, the effect of increase of the signal with the number of cycles should not be determined by enzyme kinetics.…”

Interaction of femtosecond laser radiation with skin: mathematical model

“…Фемтосекундное излучение высокой мощности применяется для надрезов и удаления поверхностных и подповерхностных тканей, в том числе на коже [3] и в глазах [4,5]. Фундаментальные и прикладные исследования с использованием фемтосекундного излучения в настоящее время направлены на совершенствование методов нелинейной микроскопии [6,7], разработку новых методов оптического сверхразрешения и увеличения поля зрения оптических систем [8,9], на исследование особенностей распространения лазерного излучения в оптиче-ски нелинейных средах [10][11][12][13] и особенностей генерации фемтосекундного спектрального суперконти-нуума [14,15], на изучение влияния на молекулярную динамику [16,17] и воздействие излучения на от-дельные молекулы (лазерный пинцет) [18]. Известны работы по исследованию воздействия спиральных лазерных пучков фемтосекундной длительности на клетки [19], биопринтингу [20,21], решаются задачи управления ДНК [22].…”