The pervasive presence of electromagnetic fields (EMF) generated by modern technologies poses a significant threat to human health, with ionizing radiation, a byproduct of EMF, potentially contributing to cancer development. This study explores the impact of chronic exposure to GSM-EMFs and 900-1800 MHz-induced electromagnetic radiation (EMR) on liver enzymes, and serum electrolytes in mice and potential mitigating effect of exogenous glutathione administration. Thirty-five adult male mice were randomly divided into seven groups and exposed to various modes of mobile phone radiation for five weeks, with or without glutathione administration. Liver enzymes (ALP, ALT, AST) and serum electrolytes (sodium, potassium, bicarbonate) were analyzed. Results showed significant increases in ALP levels in the Silent, Ringtone + GSH, and Silent + GSH groups compared to controls, while ALT and AST levels remained largely unchanged. Serum electrolyte concentrations did not significantly differ across experimental groups. Overall, the study underscores the need for continued research to better understand the health implications of EMF exposure and inform public health measures.