There are many sources to increase value in an integrated mining–metallurgy context. The optimal strategy for concentration of minerals from the ore is one of these sources. The elements of mining-metallurgy operations from resources, infrastructure, mining site, processing, and metallurgy are mutually dependent, and reinforce and interact positively with each other to generate a combined value greater than the sum of their individual contributions. Bioleaching technology is an alternative processing technology for treatment of low-grade sulphide ore, and its application contributes to the achievement of better economic results than the conventional pyrometallurgical technology. This work presents the application of bioleaching technology in laboratory conditions for metal extraction from the polymetallic deposit Tenka in East Serbia, belonging to the company Serbia Zijin Bor Copper Doo (formerly the Mining Smelter Basin Bor Group). Bioleaching of sulphide polymetallic raw material is performed by chemolithoautotrophic microorganisms. Applying the bioleaching technology, the achieved recoveries of metals from the polymetallic ore are as follows: Cu 90%, Au 90%, Ag 80%, Zn 72% and Pb 90%. The calculated values for Net Present Value (NPV) and Internal Rate of Return (IRR) are $ 21.927 million and 12.55%, respectively.