2020
DOI: 10.3389/fbioe.2020.00277
|View full text |Cite
|
Sign up to set email alerts
|

Computer-Aided Rational Design of Efficient NADPH Production System by Escherichia coli pgi Mutant Using a Mixture of Glucose and Xylose

Abstract: Lignocellulosic biomass can be hydrolyzed into two major sugars of glucose and xylose, and thus the strategy for the efficient consumption of both sugars is highly desirable. NADPH is the essential molecule for the production of industrially important valueadded chemicals, and thus its availability is quite important. Escherichia coli mutant lacking the pgi gene encoding phosphoglucose isomerase (Pgi) has been preferentially used to overproduce the NADPH. However, there exists a disadvantage that the cell grow… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

1
1
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
4

Relationship

0
4

Authors

Journals

citations
Cited by 4 publications
(2 citation statements)
references
References 60 publications
(80 reference statements)
1
1
0
Order By: Relevance
“…Interestingly, although no significant change was found in total NADP levels, PDLSCs incubated under high glucose conditions exhibited a significant reduction in NADP + levels and an increase in NADPH levels and the NADPH/NADP + ratio ( Figure 2C ), indicating an increased NADPH production in cells under high glucose conditions. These results were consistent with previous studies suggesting that glucose is a main source of NADPH production and increasing glucose concentration can acutely promote NADPH generation from NADP + and increase intracellular NADPH levels ( 51 , 52 ). Ying et al.…”
Section: Discussionsupporting
confidence: 93%
“…Interestingly, although no significant change was found in total NADP levels, PDLSCs incubated under high glucose conditions exhibited a significant reduction in NADP + levels and an increase in NADPH levels and the NADPH/NADP + ratio ( Figure 2C ), indicating an increased NADPH production in cells under high glucose conditions. These results were consistent with previous studies suggesting that glucose is a main source of NADPH production and increasing glucose concentration can acutely promote NADPH generation from NADP + and increase intracellular NADPH levels ( 51 , 52 ). Ying et al.…”
Section: Discussionsupporting
confidence: 93%
“…Because the natural cofactor NAD(P)H is widely involved in various metabolic reactions in the cell, a change in its concentration in the cell will cause disturbances to cell life activities [ 46 ]. Therefore, unnatural cofactors such as nicotinamide cytidine dinucleotides (NCD) [ 47 ] and nicotinamide mononucleotides (NMN) were designed to be coupled to oxidoreductases [ 48 ].…”
Section: Electron Transfer Pathway Engineeringmentioning
confidence: 99%