Computer-assisted semen analysis parameters in men with varicocele: is surgery helpful?

Parikh, Firuza R.; Kamat, Sudesh; Kodwaney, Geeta G.; Balaiah, D.

doi:10.1016/s0015-0282(16)58516-1

Cited by 24 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This suggested that sperm from individuals with varicocele had a lower degree of maturity, higher degree of DNA fragmentation, and altered epigenetic state. These conclusions supported reports in the literature which suggested that individuals with varicocele had reduced efficiency of spermatogenesis which accounted for reduced semen parameters [Parikh et al 1996;Pasqualotto et al 2005]. Improper replacement of histone with protamine led to relatively decondensed sperm chromatin, which increased susceptibility to ROS mediated DNA damage [Sadek et al 2011;Sakkas and Alvarez 2010].…”

Section: Parameterssupporting

confidence: 84%

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele

Bahreinian

Tavalaee

Abbasi

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

This study aimed to assess and compare global DNA methylation (GDM) between fertile men and infertile men with varicocele. In addition, we evaluated the correlations between DNA methylation with reactive oxygen species (ROS), protamine deficiency, and DNA integrity. Semen samples were collected from 44 men with grades II and III varicocele, and 15 fertile men for assessment of semen parameters, DNA methylation, DNA fragmentation, oxidative stress, and protamine deficiency. Samples were evaluated by the World Health Organization (WHO) criteria, immunostaining, the TUNEL assay, 2 0 , 7 0 -dichlorodihydrofluorescein diacetate (DCFH-DA) staining, and chromomycin A3 (CMA3) staining. Semen parameters were significantly lower in individuals with varicocele compared to fertile men. The percentage of GDM and intensity of DCFH were reduced and the percentages of DCFH, TUNEL, and CMA3 positive sperm significantly increased in individuals with varicocele compared to fertile men. Correlation analysis revealed a negative significant relation between DNA methylation and DNA fragmentation, but not with the degree of protamine deficiency and ROS production. The results have shown that individuals with varicocele show increased DNA susceptibility to damage when DNA is hypomethylated. This phenomenon appears to be independent of ROS production.

show abstract

Section: Parameterssupporting

confidence: 84%

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele

Bahreinian

Tavalaee

Abbasi

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Despite the published data by Parikh et al 20. we could not find differences in the overall sperm kinetics after varicocelectomy by using CASA.…”

Section: Discussioncontrasting

confidence: 96%

Computer-aided sperm analysis: a useful tool to evaluate patient′s response to varicocelectomy

et al. 2017

View full text Add to dashboard Cite

Preoperative and postoperative sperm parameter values from infertile men with varicocele were analyzed by computer-aided sperm analysis (CASA) to assess if sperm characteristics improved after varicocelectomy. Semen samples of men with proven fertility (n = 38) and men with varicocele-related infertility (n = 61) were also analyzed. Conventional semen analysis was performed according to WHO (2010) criteria and a CASA system was employed to assess kinetic parameters and sperm concentration. Seminal parameters values in the fertile group were very far above from those of the patients, either before or after surgery. No significant improvement in the percentage normal sperm morphology (P = 0.10), sperm concentration (P = 0.52), total sperm count (P = 0.76), subjective motility (%) (P = 0.97) nor kinematics (P = 0.30) was observed after varicocelectomy when all groups were compared. Neither was significant improvement found in percentage normal sperm morphology (P = 0.91), sperm concentration (P = 0.10), total sperm count (P = 0.89) or percentage motility (P = 0.77) after varicocelectomy in paired comparisons of preoperative and postoperative data. Analysis of paired samples revealed that the total sperm count (P = 0.01) and most sperm kinetic parameters: curvilinear velocity (P = 0.002), straight-line velocity (P = 0.0004), average path velocity (P = 0.0005), linearity (P = 0.02), and wobble (P = 0.006) improved after surgery. CASA offers the potential for accurate quantitative assessment of each patient's response to varicocelectomy.

show abstract

“…Sperm morphology is an important predictor of fertilizing potential; when the proportion of normal forms is <20%, there is complete failure of fertilization under in vitro conditions [ 21]. The reports of improvement in sperm morphology after varicocelectomy differ among studies [ 17, 20, 22]. In the present study, there were no differences in normal forms or in tail defects in sperm from those with varicoceles and from controls, but only in the amorphous forms ( P =0.02).…”

Section: Discussionmentioning

confidence: 99%

A follow‐up study of semen quality and fertility in men with varicocele testis and in control subjects

Lund

Larsen

1998

British Journal of Urology

View full text Add to dashboard Cite

Objective To evaluate semen quality and fecundity in a men with and without reduced semen quality. There were no diCerences between the groups in sperm group of men with initially untreated varicocele testis, in comparison with control subjects. motility values (straight-line velocity, curvilinear velocity) evaluated using computer-assisted semen analyPatients, subjects and methods In a prospective, longitudinal study, 77 men (39 with varicocele and 38 sis. Paternity was achieved by 10 of 14 men in the varicocele group, compared with 14 of 16 in the control subjects) were evaluated in 1989 and re-investigated after 8 years. Fifty-seven men (29 with control group. Conclusions There was no evidence that varicovaricocele, 28 controls) were available for evaluation and they all completed a questionnaire. Semen from celectomy might benefit patients, because the sperm concentration did not decline among men with varicoa total of 46 men (24 with varicocele) was compared with findings in 22 men from the control group.cele over time. Computer-assisted semen analysis provided a good assessment of various sperm motility Results There was a decline in sperm count in the control group from 80×106/mL to 55×106/mL over characteristics and we recommend evaluation using such objective methods, to allow comparison among the 8-year period, but nearly no change in the sperm count in the men with varicocele. The withdrawal studies Keywords Varicocele testis, semen, fertility, male rate from each group was the same and included both in a group with initially untreated varicocele and a

show abstract

Computer-assisted semen analysis parameters in men with varicocele: is surgery helpful?

Cited by 24 publications

References 20 publications

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele

DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele

Computer-aided sperm analysis: a useful tool to evaluate patient′s response to varicocelectomy

A follow‐up study of semen quality and fertility in men with varicocele testis and in control subjects

Contact Info

Product

Resources

About