Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4°C) on sperm quality

Filliers, Muriel; Rijsselaere, Tom; Bossaert, Philippe; Causmaecker, V. De; Dewulf, Jeroen; Pope, C. E.; Soom, Ann Van

doi:10.1016/j.theriogenology.2008.07.004

Cited by 44 publications

(35 citation statements)

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“…Motility is crucial for achieving fertilization (Palomo et al, 2016), but in the cat, it decreased significantly due to cryopreservation (Filliers et al;Choi et al, 2010). Our results support that PG was efficient for improving sperm motility parameters in frozen samples for all analyzed cats, similar to previous reports for fresh semen (Villaverde et al, 2009(Villaverde et al, , 2014.…”

Section: Discussionsupporting

confidence: 90%

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Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

2017

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CHEUQUEMÁN, C.; SÁNCHEZ, R. & RISOPATRÓN, J.Effect of sperm selection techniques in frozen/thawed cat spermatozoa on sperm motility analyzed by CASA system. Int. J. Morphol., 35(4):1495Morphol., 35(4): -1501Morphol., 35(4): , 2017. SUMMARY:Freeze/thawing process reduces sperm survival and fertilizing ability of cat spermatozoa, with sperm motility being the most sensitive sperm parameter altered, due to cryo-damage. In this context, swim-up and density gradient processing methods can help to recover high motile and normal spermatozoa. Maximizing the use of frozen semen sample is essential, especially in endangered felids or high value cats in which sample size, number of samples or access to semen collection is reduced. To our knowledge, there is no previous report describing an in depth analysis of sperm motility improvement, after sperm selection techniques in frozen cat semen. Accordingly, we evaluated the effect of percoll gradient (PG) and swim up (SU) sperm selection techniques on sperm motility parameters and sperm recovery rate in frozen/thawed spermatozoa of domestic cat. Next, we evaluated the individual effect of the cat over sperm motility after PG sperm selection of frozen/thawed spermatozoa. SU and PG improved significantly all sperm motility parameters of frozen/thawed cat spermatozoa compared to simple washing. However, PG allows better sperm recovery from the original frozen sample and works mostly homogeneously among individual cats. This new information could help to maximize the use of frozen semen in endangered felids or high value domestic cats for its subsequent application on in vitro fertilization and artificial insemination.

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Section: Discussionsupporting

confidence: 90%

“…However, freeze/thawing process reduces sperm survival and fertilizing ability of cat spermatozoa (Luvoni, 2006;Thiangtum et al, 2009;Terrell et al, 2012), being sperm motility the most sensitive sperm parameter altered due to cryo-damage (Filliers et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

2017

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“…72(6): 777-780, 2010 The total number of animals worldwide has decreased in many wildcat species, and various assisted reproductive technologies (ART) are necessary to maintain and increase their numbers. Many studies on ART for endangered feline species are underway using domestic cats as a model [1][2][3][4][5][10][11][12][13][14][15].…”

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confidence: 99%

The Qualities of Cryopreserved Epididymal Sperm Collected from Feline Epididymides Stored at Low Temperature

Toyonaga

Sato

Morita

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. We observed the influences of low-temperature storage of the feline epididymis on the epididymal semen qualities before and after cryopreservation to identify the optimal duration for low-temperature storage of the epididymis. After excision, the feline epididymis was stored at 4  C for 0-72 hr and then subjected to epididymal sperm collection. When sperm from the refrigerated cauda epididymis were frozen and thawed, there was no significant difference in sperm motility between the 0-and 24-hr low-temperature storage groups, but sperm motility was significantly decreased in the 48-hr storage group. The above findings suggested that low-temperature storage of the epididymis until 24 hr is useful for frozen sperm collected from the feline cauda epididymis.KEY WORDS: cryopreservation, epididymal sperm, feline, refrigeration.J. Vet. Med. Sci. 72(6): 777-780, 2010 The total number of animals worldwide has decreased in many wildcat species, and various assisted reproductive technologies (ART) are necessary to maintain and increase their numbers. Many studies on ART for endangered feline species are underway using domestic cats as a model [1][2][3][4][5][10][11][12][13][14][15].In a previous study, we collected feline sperm from the cauda epididymis immediately after excision and performed intrauterine insemination of frozen semen (5  10 6 ) [14]. The mean sperm motility following freeze-thawing was 24.0  4.0% (SE), and the conception rate was only 27.3% (3/11). Two female cats delivered five and one kittens, respectively, and another one aborted on 30 days of gestation. This was our initial success rate achieved using epididymal frozen semen in cats. Our previous study is also the only one to report successful conception cases achieved using frozen feline epididymal semen thus far.The duration and temperature of storage before collection of sperm after excision of the epididymis have marked influences on semen qualities in various animal species [6][7][8][9]. In cats, Hay and Goodrowe [5] reported that semen qualities did not differ between sperm collected from the cauda epididymis immediately after excision and after 24-hr storage at 5C. Furthermore, Chatdarong et al. [3] compared the quality of caudal epididymal semen collect from the epididymis after storage for 2 and 4 days at 4C and reported that the sperm motility after storage for 2 days is significantly higher than that after storage for 4 days. Filliers et al. [4] observed that the qualities of semen from the feline epididymis stored in sterile physiological saline for 24 hr at 5C were good. However, Chatdarong et al. [3] and Filliers et al. [4] did not observe the semen quality immediately after excision. Tittarelli et al. [13] also stored the feline epididymis in sterile physiological saline or egg yolk-Tris for 24, 48 and 72 hr and observed the semen qualities. Sperm motility was apparently decreased at all storage time points, and egg yolk-Tris was suggested to be superior as a storage medium compared with physiological saline. Howev...

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“…Epididymal spermatozoa have been harvested from a variety of species like cats (Filliers et al, 2008;Hermansson & AxnÈr, 2007), dogs (Garcia-Macias et al, 2006;Hewitt et al, 2001;Nothling et al, 2007;Ponglowhapan et al, 2006), rats (Yamashiro et al, 2007), horses (Braun et al, 1994;Bruemmer, 2006;Heise et al, 2011;Johnson & Coutinho da Silva, 2008;Melo et al, 2008), cattle (Goovaerts et al, 2006;Martins, Rumpf, Pereira, & Dode, 2007), pigs (Ikeda et al, 2002), sheep (Garcia-Macias et al, 2006), goats (Blash, Melican, & Gavin, 2000), red deer (Fernandez-Santos et al, 2006;Garcia-Macias et al, 2006;MartÌnez-Pastor et al, 2006), Spanish Ibex , African buffalo (Herold et al, 2006), North American buffalo (Lessard at al., 2009) and monkeys (Goff et al, 2009;Ng et al, 2002). Pregnancies and offspring after AI with epididymal spermatozoa have been produced amongst others in horses (Barker & Gandier, 1957;Heise et al, 2010;Papa et al, 2008), dogs (Hori, Hagiuda, Kawakami, & Tsutsui, 2005) and Spanish Ibex (SantiagoMoreno et al, 2006).…”

Section: Use Of Epididymal Spermatozoa For Aimentioning

confidence: 99%

Artificial Insemination in Veterinary Science

Heise¹

2012

A Bird's-Eye View of Veterinary Medicine

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Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4°C) on sperm quality

Cited by 44 publications

References 66 publications

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

Effect of Sperm Selection Techniques in Frozen/Thawed Cat Spermatozoa on Sperm Motility Analyzed by CASA System

The Qualities of Cryopreserved Epididymal Sperm Collected from Feline Epididymides Stored at Low Temperature

Artificial Insemination in Veterinary Science

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