Computer-Assisted Sperm Analysis of Head Morphometry and Kinematic Parameters in Warmblood Stallions Spermatozoa

Křížková, Jana; Čoudková, Veronika; Maršálek, Miroslav

doi:10.1016/j.jevs.2017.05.012

Cited by 11 publications

(6 citation statements)

References 47 publications

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“…Kathiravan et al (2011) reported that analysis of sperm motility using CASA system is more effective and precise for fertility assessment than subjective evaluation among different breeds and age categories of breeding males. Studies on the use of CASA measurements exhibiting significant correlations between motility and fertility have been conducted in bovine (Gillan et al 2008, Kumar et al 2015, equine (Køí•ková et al 2017), human being (Hirano et al 2001), rabbit (Lavara et al 2005), ovine (Palacin et al 2013) and swine (Broekhuijse et al 2011a). https://doi.org/10.56093/ijans.v91i6.115435…”

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confidence: 99%

Computer-assisted sperm analysis (CASA) in veterinary science: A review

Singh

Kumar

Bisla

2021

Indian J of Anim Sci

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Computer-assisted sperm analysis (CASA) allows an assessment of sperm motion and morphology more accurately and objectively than by subjective evaluation. Although, CASA instruments have improved significantly during last 40 years especially in terms of software, image capture and computer settings, little has changed regarding processes for analyzing sperm motion attributes. The main problem is related to validation, consistency and optimization of equipment and procedures. Differences among CASA systems denote problems of objective analysis of results between different semen processing units. If validated, CASA systems can provide a great tool to objectively compare sperm motility and morphology. Sperm motility is one of the indicators most evaluated before and after cryopreservation vis-à-vis quality and fertilizing ability. Researchers have determined a possible relationship of CASA outputs with bull fertility in vivo; however, a clear association has not yet been confirmed. Most CASA measures depend upon concentration, sample volume, type of extender, duration of analysis and thawing temperature. For each attribute, CASA software should provide outputs based on a range rather than means or medians for transformed data. The current review describes development, validation requirements, limitations and future expansions associated with CASA technology.

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confidence: 99%

Computer-assisted sperm analysis (CASA) in veterinary science: A review

Singh

Kumar

Bisla

2021

Indian J of Anim Sci

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“…[37], with similar mean for all kinetic parameters (VCL 63 23. μm/s, VSL 31.02 μm/s, VAP 47.53 μm/s, STR 65.24%, LIN 48.8%).…”

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confidence: 64%

A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen

et al. 2019

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Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted and cryopreserved into three experimental extender groups: BotuCrio ® , lactose-extender supplemented with egg yolk (20%) and lactose-extender supplemented with jenny colostrum (20%). The results demonstrated that lactose-jenny colostrum samples displayed significantly higher values in almost all parameters evaluated (p < 0.05) compared with the other two extenders after thawing (BotuCrio ® and lactose-egg yolk based extender, respectively)-Total Motility, Viability, HOS test, VCL, VSL and VAP. Acrosome status, LIN, STR and WOB despite showing lower values, none of them were statistically significant (p > 0.05). In conclusion, the extender containing jenny colostrum can be successfully used for donkey semen crypreservation and could effectively improve donkey sperm qualities after freezing-thawing.

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“…The Bali-polled bull had a good value of kinematic sperms (Table 4), according to the statement Krízková et al (2017) VCL values can be divided into: fast (>90 μm/s), moderate (45-90 μm/s), slow (10-45 μm/s), and statis or immotile (<10 μm/s). VAP values> 25.0 μm/s are a good predictor of in vitro fertilization ability (Suzuki et al, 2003).…”

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confidence: 99%

Characteristic and Kinematics of Bali-Polled Bull Sperms

Diansyah¹,

Yusuf²,

Toleng³

et al. 2022

AAVS

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The purpose of this study was to determine the characteristics and kinematics of Bali-polled bull sperm to identify reproductive potential. Semen collection of Bali-polled bull was conducted once a week using an artificial vagina. The semen was proceeded until the frozen semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by using CASA with the Sperms Vision Version 3.7.5 program. The results in this study showed that sperms motility in Bali-horned bull was significantly lower (P<0.01) than Bali-polled bull, Brahman, Limousine, and Simmental. The sperms viability in Bali-horned and polled bull was significantly lower (P<0.01) compared to other bull breeds. The membrane integrity and acrosome integrity of Bali-polled bull sperms was significantly higher (P<0.01) than in Bali-horned bull. DCL, DAP, and DSL of Bali-polled bull were significantly higher (P<0.01) than Bali-horned bull. VCL, VAP, and VSL of Bali-horned bull were significantly lower (P<0.01) than Bali-polled, Simmental, Limousine, and Brahman bull. Based on the results of this study, it can be concluded that Bali-polled bull had good sperm characteristics and kinematics compared with various breeds so that the Bali-polled bull can have the potential to become superior bulls to contribute to the strategies for developing Bali bull as a national beef bull contributor.

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Computer-Assisted Sperm Analysis of Head Morphometry and Kinematic Parameters in Warmblood Stallions Spermatozoa

Cited by 11 publications

References 47 publications

Computer-assisted sperm analysis (CASA) in veterinary science: A review

Computer-assisted sperm analysis (CASA) in veterinary science: A review

A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen

Characteristic and Kinematics of Bali-Polled Bull Sperms

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