Computer Controlled High-Throughput Integration System: FasTEM

Fukushima, Kurio; O’Donnell, Ryan M.; Fujiwara, Kazunari; Kai, He; Okunishi, Eiji; Kawasaki, Masahiro; Kersker, M.; Naruse, Mikio

doi:10.1017/s1431927600038216

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All development and testing were carried out on a JEM‐2010F and a JEM‐3000SFF (JEOL USA, Peabody, MA), 200‐ and 300‐keV transmission electron microscopes. These microscopes are under computer control managed by JEOL's fastem 3.00 application (Fukushima et al ., 2000). fastem is a client/server application, enabling the microscopist to use a remote fastem client as a graphical microscope control interface that delegates directives across the network to the fastem server connected directly to the microscope.…”

Section: Methodsmentioning

confidence: 99%

“…Unfortunately, the software to run these components is rarely shared between companies, resulting in partially integrated components, supporting only limited experimental tasks. In particular we found no available solution for low‐dose imaging that integrated software control of our Gatan CCD cameras with the fastem software package (Fukushima et al ., 2000) that controls our JEOL microscopes. To meet our needs but also establish a more general solution, we found it necessary to develop a package that handles communication between electron microscope components and provides researchers with a single interface to their equipment for software engineering.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Modular software platform for low‐dose electron microscopy and tomography

Marsh

Chang

Booth

et al. 2007

Journal of Microscopy

View full text Add to dashboard Cite

SummaryTransmission electron microscopy imaging protocols required by structural scientists vary widely and can be laborious without tailor-made applications. We present here the JEOL AUTOMATED MICROSCOPY EXPERT SYSTEM (JAMES) API INTEGRATOR, a programming library for computer control of transmission electron microscopy operations and equipment. JAMES has been implemented on JEOL microscopes with Gatan CCDs but is designed to be modular so it can be adapted to run on different microscopes and detectors. We have used the JAMES API INTEGRATOR to develop two applications for lowdose digital imaging: JAMES imaging application and the MR T tomographic imaging application. Both applications have been widely used within our NCRR-supported Center for routine data collection and are now made available for public download.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modular software platform for low‐dose electron microscopy and tomography

Marsh

Chang

Booth

et al. 2007

Journal of Microscopy

View full text Add to dashboard Cite

show abstract

“…Through the development and improvement of commercial remote PC operation systems, this means that microscope remote operation is now identical to local operation and lends itself to a number of applications: remote operation for enhanced performance, instrument sharing, experiment collaboration, remote diagnostics, remote servicing, and remote teaching and training. 1,2 The JEOL JEM-ARM200F TEM can be remotely operated in just this fashion. A remote station is connected to the microscope and accessory computers through standard remote operating protocols over an Internet connection.…”

mentioning

confidence: 99%

Practical Remote Electron Microscopy - A Vendor's Perspective

et al. 2011

View full text Add to dashboard Cite

As microscopes have become more complex, they have become more specialized. Due to the expense of these instruments, not every laboratory that has a need for a specific feature on a microscope can afford such a machine. This has led to an increase in collaborative funding for instrumentation, with the instrument residing in one centralized facility. With this increased instrument complexity comes an increased sensitivity to the local environment of the microscope. For environmental effects such as thermal fluctuations, pressure fluctuations, air flow, mechanical vibrations and acoustic noise the operator is a major source. Remote operation of these instruments allows the microscope to be placed in the best possible environment while the operator can remain separated, in a comfortable environment. This ensures that the newest generation of microscopes can achieve their full potential.Practical remote operation of electron microscopes has existed commercially for more than a decade. These early iterations often involved using software different from the microscope control software, as well as condensed versions of hardware, if any hardware at all. This meant that remote operation could be somewhat limited, with access to all features of the microscope not possible. This also meant that a remote operator needed to learn a new way to operate the microscope, as these remote versions were not the same as sitting in front of the instrument to operate it.Current electron microscopes feature fully digital components and are operated by personal computers running standard operating systems. Through the development and improvement of commercial remote PC operation systems, this means that microscope remote operation is now identical to local operation and lends itself to a number of applications: remote operation for enhanced performance, instrument sharing, experiment collaboration, remote diagnostics, remote servicing, and remote teaching and training. 1,2The JEOL JEM-ARM200F TEM can be remotely operated in just this fashion. A remote station is connected to the microscope and accessory computers through standard remote operating protocols over an Internet connection. Microscope control knobsets and a trackball for stage movement are connected via USB to the remote station, making remote operation of the ARM200F identical to operating the TEM locally.JEOL SEMs use similar remote operating systems, such as VNC (Virtual Network Computing) software to allow a remote PC to log on to the SEM via an internet browser to have both live remote viewing and/or live remote control of the SEM. The real time lag in response can be as short as a few hundred milliseconds or as long as several tens of seconds depending on many factors such as: internet traffic, speed of any dedicated lines, and internal network bridging and security on both ends of the connection. VNC consists of two components. A server, which runs on the computer you want to remotely access (the SEM PC), and a viewer, which runs on the computer you are sitting in front...

show abstract

“…Full remote control of TEMs has been a reality for nearly a decade. 2 One system for remotely operating these instruments is Sirius. Sirius is a client / server platform that interfaces directly with the control software of the TEM.…”

mentioning

confidence: 99%

Remote Teaching for Electron Microscopy

et al. 2008

View full text Add to dashboard Cite

Modern electron microscopes feature fully digital components and are operated by personal computers running standard operating systems. A benefit of this operation is that these instruments can be controlled by a computer sitting next to the instrument, or by one connected to the instrument via a local network (Intranet) or by the Internet. This remote operation is identical to local operation and lends itself to a number of applications: remote operation for enhanced performance, instrument sharing, experiment collaboration, remote diagnostics, remote servicing, and remote teaching and training. 1As microscopes have become more complex, they have become more specialized. Due to the expense of these instruments, not every laboratory that has a need for a specific feature on a microscope can afford such a machine. This has led to an increase in collaborative funding for instrumentation, with the instrument residing in one centralized facility. This means that teaching of electron microscope fundamentals as well as training for the instruments is not necessarily limited to a single facility. Through remote operation of the microscope, users and students at remote facilities can get the education and training they need without the added expense of travel.Classroom teaching of electron microscopy has typically been the teaching of the theory and operation of these instruments without the benefit of actually operating one during class. The electron microscope often resides in an environmentally controlled laboratory that is not conducive to a large number of students being near by for education and training. Remote operation eliminates this problem by instead bringing the instrument to the students.Full remote control of TEMs has been a reality for nearly a decade. 2 One system for remotely operating these instruments is Sirius. Sirius is a client / server platform that interfaces directly with the control software of the TEM. The remote software terminal is designed to work with a trackball and knob set so that remote microscope operation is completely identical to local operation ( Figure 1). Sirius operates using the TCP/IP protocol, with the remote client interfacing directly to a server. This makes the connection secure, and ensures that only one client (and thus one user) can be connected to the microscope at any given time. All operation and data collection is integrated by combining Sirius with a remote connection to a PC operating the Gatan Microscopy Suite (Figure 2). The system sends small compressed data communications to conserve network bandwidth, resulting in live time operation.

show abstract

Computer Controlled High-Throughput Integration System: FasTEM

Cited by 3 publications

References 0 publications

Modular software platform for low‐dose electron microscopy and tomography

Modular software platform for low‐dose electron microscopy and tomography

Practical Remote Electron Microscopy - A Vendor's Perspective

Remote Teaching for Electron Microscopy

Contact Info

Product

Resources

About