Computing Method and Test on IC50 Value of Tyrosinase Inhibition

Abstract

Calculating methods of inhibitor concentration leading to yield 50% inhibition (IC50) of competitive inhibitor, noncompetitive inhibitor and mixed inhibitor were proposed on the basis of kinetics of enzyme inhibition, and were applied to calculate IC50 value of concerned compounds, such as cinnamic acid, cinnamaldehyde, benzoic acid and et al. The calculating results of competitive inhibitor, noncompetitive inhibitor and mixed inhibitor are similar to experimental data, and the average errors are only 3.7%, 2.4%, 4.6%, respectively. The calculating method is confirmed to be reliable by the results.