Concanavalin-A displays leishmanicidal activity by inducing ROS production in human peripheral blood mononuclear cells

Thomazelli, Ana Paula Fortes dos Santos; Tomiotto-Pellissier, Fernanda; Miranda-Sapla, Milena Menegazzo; Silva, Suelen Santos da; Alvarenga, Daniele Sapede; Cataneo, Allan Henrique Depieri; Bordignon, Juliano; Silveira, Guilherme Ferreira; Lioni, Lucy Megumi Yamauchi; Sá, Jussevania Pereira Santos Rubro de; Felipe, Ionice; Pavanelli, Wander Rogério; Conchon‐Costa, Ivete

doi:10.1080/08923973.2018.1510960

Cited by 10 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, exogenously added AA and DHA were shown to promote ROS production in L. donovani promastigotes (Mathur et al, 2015). The production of ROS has been observed in macrophages infected with Leishmania amazonensis , Leishmania braziliensis , most likely as a defense mechanism to eliminate parasites (Carneiro et al, 2016; Reybier et al, 2010; Roma et al, 2016; Thomazelli et al, 2018). Our data consistently show increased ROS production in both MON‐24 and MON‐1 infected macrophages.…”

Section: Discussionmentioning

confidence: 99%

Enriched PUFA environment of Leishmania infantum promastigotes promotes the accumulation of lipid mediators and favors parasite infectivity towards J774 murine macrophages

Leroux

Messaoud

Luquain-Costaz

et al. 2022

Lipids

View full text Add to dashboard Cite

Leishmania parasites are the causative agents of visceral or cutaneous leishmaniasis in humans and of canine leishmaniosis. The macrophage is the predilected host cell of Leishmania in which the promastigote stage is transformed into amastigote. We previously showed changes in the fatty acid composition (FA) of lipids in two strains of Leishmania donovani upon differentiation of promastigote to amastigote, including increased proportions of arachidonic acid (AA) and to a less extent of docosahexaenoic acid (DHA). Here, we carried out supplementation with AA or DHA on two Leishmania infantum strains, a visceral (MON-1) and a cutaneous (MON-24), to evaluate the role of these FA in parasite/macrophage interactions. The proportions of AA or DHA in total lipids were significantly increased in promastigotes cultured in AA-or DHAsupplemented media compared to controls. The content of FA-derived oxygenated metabolites was enhanced in supplemented strains, generating especially epoxyeicosatrienoic acids (11,(12)(13)(14) and hydroxyeicosatetraenoic acids (5-and 8-HETE) from AA, and hydroxydocosahexaenoic acids (14and 17-HDoHE) from DHA. For both MON-1 and MON-24, AA-supplemented promastigotes showed higher infectivity towards J774 macrophages as evidenced by higher intracellular amastigote numbers. Higher infectivity was observed after DHA supplementation for MON-24 but not MON-1 strain. ROS production by macrophages increased upon parasite infection, but only minor change was observed between control and supplemented parasites. We

show abstract

Section: Discussionmentioning

confidence: 99%

Enriched PUFA environment of Leishmania infantum promastigotes promotes the accumulation of lipid mediators and favors parasite infectivity towards J774 murine macrophages

Leroux

Messaoud

Luquain-Costaz

et al. 2022

Lipids

View full text Add to dashboard Cite

show abstract

“…ConA extracted from Canavalia ensiformis displays antiprotozoal activity at 0.39 μM against L. amazonensis . The ConA treatment eliminated promastigotes in a post-infection period and increased the expressions of cytokines such as IFN-γ, IL-6, TNF- α, IL-4, IL-2, and IL-10 to overcome L. amazonensis infection [ 21 ]. These findings suggest that inhibitors may be developed for infectious diseases using lectins that may bind to specific cell surface glycans.…”

Section: Lectins As Inhibitorsmentioning

confidence: 99%

Lectins as the prominent potential to deliver bioactive metal nanoparticles by recognizing cell surface glycans

Bala Subramaniyan,

Veerappan

2024

Heliyon

View full text Add to dashboard Cite

Microgramma vacciniifolia Frond Lectin: In Vitro Anti-leishmanial Activity and Immunomodulatory Effects Against Internalized Amastigote Forms of Leishmania amazonensis

de Souza Aguiar,

de Moraes Alves,

Sobrinho Júnior

et al. 2023

Acta Parasit.

View full text Add to dashboard Cite

Concanavalin-A displays leishmanicidal activity by inducing ROS production in human peripheral blood mononuclear cells

Abstract: Based on the data obtained, it was possible to infer that Con-A induces the ROS production, responsible for eliminating parasites in addition to regulatory cytokines synthesis which are important for disease resolution.

Cited by 10 publications

References 35 publications

Enriched PUFA environment of Leishmania infantum promastigotes promotes the accumulation of lipid mediators and favors parasite infectivity towards J774 murine macrophages

Enriched PUFA environment of Leishmania infantum promastigotes promotes the accumulation of lipid mediators and favors parasite infectivity towards J774 murine macrophages

Lectins as the prominent potential to deliver bioactive metal nanoparticles by recognizing cell surface glycans

Microgramma vacciniifolia Frond Lectin: In Vitro Anti-leishmanial Activity and Immunomodulatory Effects Against Internalized Amastigote Forms of Leishmania amazonensis

Contact Info

Product

Resources

About