Concanavalin A Disrupts the Release of Fibrous Material Necessary for Zygote Formation of a Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex

Abstract

The Closterium peracerosum–strigosum–littorale (C. psl.) complex is the best characterized charophycean alga with respect to the processes of sexual reproduction. We examined the effect of concanavalin A (Con A) on physiological and ultrastructural changes during the conjugation of the C. psl. complex. Two heterothallic gametangial cells formed a sexual pair as usual; however, the release of gametes was completely blocked by the addition of Con A. Fluorescein isothiocyanate-labeled Con A bound to the outermost layer of the conjugation papillae of paired cells. In the absence of Con A, the disruption of outer cell walls on the conjugation papillae and the secretion of fibrous materials from the conjugation papillae were observed using a transmission electron microscope, but Con A-treated cells did not show these changes. Instead, a highly electron-dense layer was observed in the outermost papillae, and the excess fibrous materials remained at the inside of the layer. These results suggest that an unknown molecule(s) recognized by Con A is essential for the diffusion of fibrous materials at the conjugation papillae, which is an indispensable step for gamete release during conjugation of the C. psl. complex.

Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy

“…Concavalin A strongly labelled papillae of conjugating Closterium sp. (Abe et al 2016). In contrast, papillae in Zygnema cruciatum were only weakly labelled by CA and RCA (Kim et al 2007).…”

“…This suggests the presence of one or more unknown cell wall compounds (glycoprotein and/or poly-/oligosaccharide), which only emerge during the conjugation process. Further studies may mine recently published Zygnematophyceae genomes for the repertoire of glycosyltransferases, potentially discovering new cell wall components that are crucial for sexual processes, as these components might mediate the recognition and adhesion between conjugating filaments (Kim et al 2007;Abe et al 2016). This may also address the question, whether small differences in the cell wall composition of papillae as recognized by lectins help avoiding the establishment of conjugation tubes between incompatible species and thus prevent wasting metabolic energy by building non-functional conjugation tubes.…”