Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy

Cantelli, Andrea; Piro, F.; Pecchini, Pietro; Giosia, Matteo Di; Danielli, Alberto; Calvaresi, Matteo

doi:10.1016/j.jphotobiol.2020.111852

Cited by 47 publications

(45 citation statements)

References 57 publications

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“…Regarding protein conjugates, Cantelli et al recently used carbodiimide chemistry to randomly conjugate RB to the homo-tetrameric lectin ConA [ 173 ] with an average addition of 2.4 PS per targeting ligand, and a 10-fold higher superoxide generation compared to the model compound. With improved recognition of LPS in the Gram− bacterial membrane, it showed a 4.5-fold higher uptake and greater photodynamic effect.…”

Section: Recent Studies On Targeted Apdtmentioning

confidence: 99%

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

2020

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Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally invasive manner. In photodynamic therapy (PDT), a non-toxic photosensitizer (PS) is activated by a specific wavelength of light and generates highly cytotoxic reactive oxygen species (ROS) such as superoxide (O2−, type-I mechanism) or singlet oxygen (1O2*, type-II mechanism). Although it offers many advantages over conventional treatment methods, ROS-mediated microbial killing is often faced with the issues of accessibility, poor selectivity and off-target damage. Thus, several strategies have been employed to develop target-specific antimicrobial PDT (aPDT). This includes conjugation of known PS building-blocks to either non-specific cationic moieties or target-specific antibiotics and antimicrobial peptides, or combining them with targeting nanomaterials. In this review, we summarise these general strategies and related challenges, and highlight recent developments in targeted aPDT.

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Section: Recent Studies On Targeted Apdtmentioning

confidence: 99%

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

2020

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“…The different killing efficiency between POS/ICG and POS-UCNPs/ICG demonstrated that the introduction of UCNPs would converse 808 nm NIR to the green light, thus triggering POS NSs to decompose H 2 O to generate O 2 for aPDT enhancement (Figure 5 C). On the contrary, E. coli in the POS/ICG group and POS-UCNPs/ICG groups showed similar reduction of less 2 orders of magnitude compared with control group (Figure 5 D), since Gram-negative bacteria possessed outer cell membrane containing LPS that blocked connection PSs with cell membrane protected the Gram-negative bacteria from ROS attacking 52 , 53 . The similar results were also found in the dead/live staining assay (Figure 5 E).…”

Section: Resultsmentioning

confidence: 88%

Antibacterial PDT nanoplatform capable of releasing therapeutic gas for synergistic and enhanced treatment against deep infections

et al. 2022

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Antibacterial photodynamic therapy (aPDT) has emerged as an attractive treatment option for efficient removal of pathogenic bacteria. However, aPDT in deep tissue will encounter difficulties such as limited light penetration depth, insufficient oxygen (O 2 ) supply and inability to eliminate inflammation introduced by bacteria, which hinders its clinical application. Herein, the near infrared (NIR) strategy of simultaneously generating O 2 and CO was developed for aPDT based antibacterial therapy and mitigation of deep infection inflammation. Methods: We prepared NIR-mediated multifunctional aPDT nanoplatform (POS-UCNPs/ICG) producing therapeutic gas of O 2 and CO. The CO, O 2 and ROS generation of the nanoplatform were characterized by dye probes, respectively. The antibacterial activity and anti-inflammation of POS-UCNPs/ICG were demonstrated in vitro and in vivo . In addition, the therapeutic effects in vivo were serially analyzed by immunofluorescence staining, Masson's staining, hematoxylin and eosin staining, colony formation units (CFU) and so on. Results: NIR-mediated multifunctional aPDT nanoplatform was realized by combining the up-conversion nanoparticles (UCNPs) and partially oxidized SnS 2 (POS) nanosheets (NSs) as well as indocyanine green (ICG). Using a single 808 nm light, aPDT can be achieved via ICG molecules, meanwhile, O 2 /CO can be generated by POS NSs through upconversion light excitation. During the aPDT process, O 2 can enhance aPDT, while CO can regulate inflammation through the PI3K/NF-κB pathway. Therefore, POS-UCNPs/ICG groups had a highest percentage of healing area up to 91.55±1.26% in mouse abscess model. Conclusion: Due to enhanced aPDT and anti-inflammatory collaborative therapy, the POS-UCNPs/ICG composites showed remarkably accelerated recovery in animal abscess models. Such NIR light responsive nanoplatform with optimized antibacterial capacity and immunomodulatory functions is promising for clinical therapeutics of bacteria-induced infections.

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“…The interaction with proteins may affect the photophysical properties of photosensitizers, [ 56 , 57 , 58 , 59 , 60 , 61 ] so we evaluated the BGNP performances in a physiological-like conditions, investigating the possible effects of the biomolecular corona during laser the irradiation/heating process [ 44 ]. We repeated the irradiation experiment for the FBS-dispersed BGNPs, using the highest concentration of BGNPs (0.25 mM Au 0 BGNPs).…”

Section: Resultsmentioning

confidence: 99%

Spiky Gold Nanoparticles for the Photothermal Eradication of Colon Cancer Cells

et al. 2021

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Colorectal cancer (CRC) is a widespread and lethal disease. Relapses of the disease and metastasis are very common in instances of CRC, so adjuvant therapies have a crucial role in its treatment. Systemic toxic effects and the development of resistance during therapy limit the long-term efficacy of existing adjuvant therapeutic approaches. Consequently, the search for alternative strategies is necessary. Photothermal therapy (PTT) represents an innovative treatment for cancer with great potential. Here, we synthesize branched gold nanoparticles (BGNPs) as attractive agents for the photothermal eradication of colon cancer cells. By controlling the NP growth process, large absorption in the first NIR biological window was obtained. The FBS dispersed BGNPs are stable in physiological-like environments and show an extremely efficient light-to-heat conversion capability when irradiated with an 808-nm laser. Sequential cycles of heating and cooling do not affect the BGNP stability. The uptake of BGNPs in colon cancer cells was confirmed using flow cytometry and confocal microscopy, exploiting their intrinsic optical properties. In dark conditions, BGNPs are fully biocompatible and do not compromise cell viability, while an almost complete eradication of colon cancer cells was observed upon incubation with BGNPs and irradiation with an 808-nm laser source. The PTT treatment is characterized by an extremely rapid onset of action that leads to cell membrane rupture by induced hyperthermia, which is the trigger that promotes cancer cell death.

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Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy

Cited by 47 publications

References 57 publications

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy

Antibacterial PDT nanoplatform capable of releasing therapeutic gas for synergistic and enhanced treatment against deep infections

Spiky Gold Nanoparticles for the Photothermal Eradication of Colon Cancer Cells

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