1999
DOI: 10.1016/s0014-5793(99)00072-1
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Concatemerization of tRNA molecules in the presence of trivaline derivative

Abstract: The interaction of tRNA with trivaline dansyl hydrazide trifluoroacetate (DHTV) has been studied. The shape of curves of fluorimetric titration of tRNA with DHTV and vice versa can be explained only by formation of DHTV dimers on tRNA molecules, and subsequent association of DHTV-saturated tRNA molecules with each other. The ability of tRNA molecules to form concatemers in solution in the presence of DHTV has been demonstrated by electron microscopy. Electron microscopy of the tRNA-DHTV complexes stained with … Show more

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Cited by 4 publications
(2 citation statements)
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“…The second model suggests that an increase of the DNA concentration provokes a TPT‐mediated intermolecular DNA interaction. The resulting complex should be characterized by the larger value of specific FLD 390 signal of bound TPT than those for TPT molecules adsorbed on the single DNA molecules 28, 29. DNA‐TPT binding curves were further obtained by titration of DNA with the TPT aliquots and detection of the intensity of the band at 390 nm in the FLD spectra in order to make a choice between these two models of TPT‐DNA interaction.…”
Section: Resultsmentioning
confidence: 99%
“…The second model suggests that an increase of the DNA concentration provokes a TPT‐mediated intermolecular DNA interaction. The resulting complex should be characterized by the larger value of specific FLD 390 signal of bound TPT than those for TPT molecules adsorbed on the single DNA molecules 28, 29. DNA‐TPT binding curves were further obtained by titration of DNA with the TPT aliquots and detection of the intensity of the band at 390 nm in the FLD spectra in order to make a choice between these two models of TPT‐DNA interaction.…”
Section: Resultsmentioning
confidence: 99%
“…On the other hand, the DNA complexes where the ligands electronic transitions are oriented nonparallel to the plane of the DNA base pares are characterized by the positive LD signals . The angles α for some of the DNA minor groove binders are known to be as follows: distamycin A, 46°; , Hechst 33258, 45°; DAPI, 43−46° . Tpt exhibits the negative LD r signal (Figure B), but the value of this signal is much less negative than those for typical intercalators …”
Section: Discussionmentioning
confidence: 98%