Concentración de los anticuerpos en contra de proteínas de las glándulas salivares de Aedes aegypti e historia de la exposición al virus del dengue en residentes de una zona endémica colombiana

Abstract

Introduction:Mosquito salivary proteins are able to induce an antibody response that reflects the level of human-vector contact. IgG antibodies against dengue virus (DENV-IgG) are indicators of previous exposure. The risk of DENV transmission is not only associated to mosquito or dengue factors, but also to socioeconomic factors that may play an important role in the disease epidemiology. Objective: To determine the effect of the presence of Aedes aegypti mosquitos in different stages in households and the history of dengue exposure on vector-human contact determined by the level of anti-salivary protein antibodies in people living in a Colombian endemic area. Materials and methods: A pilot study of 58 households and 55 human subjects was conducted in Norte de Santander, Colombia. A questionnaire for socioeconomic factors was administered and houses were examined for the presence of Ae. aegypti specimens in the aquatic stages. The level of DENVIgG antibodies (DENV-IgG), in addition to IgG and IgM anti-Ae. aegypti salivary gland extract (SGE) antibodies (SGE-IgG, SGE-IgM) were evaluated by ELISA using blood collected in filter paper. Results: We found a significant higher level of SGE-IgG antibodies in subjects living in houses with Ae. aegypti in aquatic stages. We also found a higher concentration of SGE-IgG antibodies in people exposed to DENV, a positive correlation between IgM-SGE and IgG-DENV and a negative correlation with IgG-SGE. Conclusion: Anti-salivary proteins antibodies are consistent with the presence of Ae. aegypti aquatic stages inside houses and DENV-IgG antibodies concentrations.

Antibody Responses Against Anopheles darlingi Immunogenic Peptides in Plasmodium Infected Humans

“…Serum samples from 179 participants were collected by active and passive case detection from November 2016 to October 2017 in Turbo and El Bagre as part of a malaria study in Colombia. These represent two malaria endemic areas with differing malaria incidence as measured by the Annual Parasite Index (API: confirmed cases during 1 year/population under surveillance ×1,000): the API in Turbo was 1.22 in 2016 and 0.77 in 2017, while malaria incidence in El Bagre was higher, with an API of 24.7 in 2016 and 21.3 in 2017 (Londoño-Rentería et al, 2015a ; Londono-Renteria et al, 2020 ; Montiel et al, 2020b ). Participant characteristics are described in Table 1 .…”

“…On-site malaria diagnosis was done by microscopy, then confirmed by nested PCR (nPCR) at the Universidad de Antioquia (Colombia) following methods described previously (Londoño-Rentería et al, 2015a ; Londono-Renteria et al, 2020 ). Based on these results, samples were classified as malaria positive or malaria negative, based on the presence/absence of parasites or a positive PCR result.…”

“…Our previous studies demonstrated that IgG antibodies against salivary proteins of major disease vectors correlate with the clinical presentation of dengue fever and malaria (Londono-Renteria et al, 2010 ; Londoño-Rentería et al, 2015a ; Montiel et al, 2020a ), and can be used to evaluate vector control interventions (Londono-Renteria et al, 2015c ). In fact, IgG antibodies against a salivary peptide (gSG6-P1) from a major African malaria vector has been validated as a reliable marker to measure exposure to several Anopheles spp.…”

New Immuno-Epidemiological Biomarker of Human Exposure to Aedes Vector Bites: From Concept to Applications

“…In addition, a significant higher level of anti-SGE IgG in subjects living in houses with Ae. aegypti aquatic stages was found [ 43 ].…”

“…Previous works provided data on human exposure to Aedes saliva and its relationship with dengue infection in the Americas [ 24 , 43 ]. Using the Nterm-34 kDa salivary peptide, one study aimed to evaluate the risk of dengue transmission in urban settings of Vientiane city, Lao PDR [ 28 ].…”