Concentrated extract of Prunus mume fruit exerts dual effects in 3T3-L1 adipocytes by inhibiting adipogenesis and inducing beiging/browning

Osmanthus fragrans Flavonoid Extract Inhibits Adipogenesis and Induces Beiging in 3T3-L1 Adipocytes

“…Overexpansion of the WAT disrupts the white-to-brown adipocyte ratio, prompting interest in brown adipocyte activation or the induction of white adipocyte browning to enhance energy catabolism and combat obesity [ 36 , 37 ]. Plant extracts rich in flavonoids show promise in promoting white adipocyte browning, boosting energy expenditure, countering high-fat diet-induced obesity, and improving metabolic function [ 38 , 39 ].…”

“…Excessive accumulation of lipids in adipocytes results in increased levels of intracellular ROS, which can lead to mitochondrial dysfunction by damaging proteins, membrane lipids, and nucleic acids [ 60 ]. It has been reported that both concentrated extract of Prunus mume fruit and 3-hydroxymorphinan can inhibit ROS generation in 3T3-L1 adipocytes to improve mitochondrial function [ 38 , 61 ]. Similarly, the results of the present study indicated that OFFE reduced intracellular ROS levels in a dose- and time-dependent manner, shielding mitochondria from oxidative-stress-induced harm and thereby preserving mitochondrial function.…”

“…The 3T3-L1 cell line (SCSP-5038) was acquired from Shanghai’s National Collection of Authenticated Cell Cultures, affiliated with the China Academy of Sciences (Shanghai, China). Induction of maturation in 3T3-L1 pre-adipocytes was performed with an established laboratory protocol [ 38 ]. Unless otherwise specified, varying concentrations of OFFE were incorporated into differentiation medium II throughout the differentiation process.…”

Nutraceuticals in Brown Adipose Tissue Activation

“…As observed by cell culture-based experiments, treatment of 3T3-L1 murine adipocytes with a concentrated water extract of Prunus mume fruit, rich in naringin, was able to promote expression of genes involved in mitochondrial biogenesis genes, (NAMPT, Nrf1, Nrf2, CPT1α) and in brown-like adipocyte differentiation (PGC-1α, UCP1, CIDEA, Cox7α1, Cox8b) also reducing, in parallel, reactive oxygen species abundance. These data suggested the flavanone naringin is able to favour the conversion of white to brite adipocytes, potentially representing a coumpound suitable for counteracting obesity development [ 84 ] and, interestingly, a clinical study revealed that treatment with the flavonoids naringin or hesperidin in combination with the alkaloid p-synephrine (see below) enhanced the effects of p-synephrine to increase the resting metabolic rate of the participants [ 85 ].…”

Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease

“…Extracts of the fruit of Prunus mume also have been used for a long time in many culinary and medicinal preparations ( Bailly, 2020 ). Previous studies have shown that a methanolic extract of Prunus mume fruit can reduce serum UA concentration in hyperuricemia mice ( Yi et al, 2012 ), an ethanol extract of Prunus mume fruit can stimulate glucose uptake by regulating PPAR-c in C2C12 myotubes and ameliorate glucose intolerance and fat accumulation in mice fed a high-fat diet ( Shin et al, 2013 ), and a concentrated water extract of Prunus mume fruit can suppress adipogenesis in 3T3-L1 adipocytes ( Bu et al, 2021 ). Studies also have reported that Prunus mume leaf extract can reduce blood glucose levels in diabetic mice ( Lee et al, 2016 ), and Prunus mume seed extract can protect against potassium oxonate-induced hyperuricemia ( Xia et al, 2013a ).…”