Concentrated Growth Factors Can Inhibit Photoaging Damage Induced by Ultraviolet A (UVA) on the Human Dermal Fibroblasts In Vitro

Abstract

Background Photoaging is the main cause of extrinsic skin aging. Daily exposure to ultraviolet A (UVA) accelerates the process of photoaging. The present study aimed to understand the role of concentrated growth factors (CGF) on UVA irradiated human skin cells. Material/Methods We isolated and subcultured normal human dermal fibroblasts (NHDFs) from 6 different human dorsal skins and established photoaging models of NHDFs irradiated by UVA to detect the influence of CGF on fibroblasts in vitro . Three groups were examined: normal, cellular photoaging model (total dosages of 18J·cm −2 ), and cellular photoaging model plus CGF. In our study, we used the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay method to measure the cell viability. We also used reactive oxygen species (ROS) assay and superoxide dismutase (SOD) assay to measure respectively the amount of oxygen free radicals and antioxidative enzymes. We compared the migration rates among the photoaging model groups, the control groups, and the CGF-treated culture medium groups that were irradiated. Results Our study results indicated that 5% CGF can reduce UVA-induced human skin fibroblasts damage significantly, improve the viability of NHDFs significantly, and largely decrease the UVA irradiation effect ( P <0.05). The migration rates of the normal group and the UVA-irradiated NHDFs in the 5% CGF group had significantly increased migration rates ( P <0.05), compared to the control medium group. The migration rates of the UVA-irradiated NHDFs in 5% CGF exceed those of the normal group. These results showed that 5% CGF could greatly promote cellular proliferation, migration, and SOD at the same time that the amounts of ROS were markedly decreased. Conclusions These experimental findings offer some important insights into CGF’s capacity for scavenging ROS, improving SOD, and increasing migration rates in NHDFs irradiated by UVA.

A Novel Autologous Topical Serum Based on Plasma Rich in Growth Factors Technology Counteracts Ultraviolet Light-Derived Photo-Oxidative Stress

“…This is consistent with other studies regarding platelet-derived products that activate detoxifying enzymes by the overexpression of the antioxidant response element [31]. In fact, one of the major antioxidant enzymes, superoxide dismutase (SOD) with free radical scavenging activity has been shown to be upregulated after growth factor concentrate treatment following UV irradiation [32]. In this line, the use of epidermal growth factor has also shown to promote the expression of catalase in response to photodamage, which is an antioxidant enzyme prominently expressed in the stratum corneum of the skin [33].…”

Concentrated growth factor inhibits UVA-induced photoaging in human dermal fibroblasts via the MAPK/AP-1 pathway

“…However, few studies have focused on the effects of CGF in repairing skin photoaging. In our previous study, we showed that CGF is capable of inhibiting UVA-induced photoaging of human dermal fibroblasts (HDFs) [ 19 ]; in the present study, we further elucidate the underlying mechanism for this CGF-mediated inhibition of UVA irradiation-induced aging.…”

“…Some studies have used a cell survival rate of 50% as the selection criterion for radiation dose [ 23 ]. Following literature and a previous study from our laboratory [ 19 ], we used 5, 10, 20, and 30 J/cm 2 of UVA to irradiate HDFs and screen the appropriate UVA irradiation dose. The results showed that the 24 h survival rate following irradiation with 20 J/cm 2 UVA was 64%, which met the requirements of the present study and thus was used as the UVA irradiation dose for the following experiment.…”

In vitro and in vivo effects of concentrated growth factor on cells and tissues

“…Only one study in this group compared CGF extract with other platelet concentrate including PRP, PRGF, and A‐PRF extract and reported more proliferation in PRP‐treated cells (Masuki et al, ). While in the study of Jun et al on CGF extracts (2, 5, 10, and 15%), a dose‐dependent manner in CGF groups was reported, study of Chen et al on the effects of 5, 10, 15, and 20% concentrations of CCM showed better effect of 5% CGF in comparison to other concentrations (Chen, Jiao, et al, ; Jun et al, ).…”

“…Only one study in this group compared CGF extract with other platelet concentrate including PRP, PRGF, and A-PRF extract and reported more proliferation in PRP-treated cells(Masuki et al, 2016). While in the study of Jun et al on CGF extracts (2, 5, 10, and 15%), a dose-dependent manner in CGF groups was reported, study of Chen et al on the effects of 5, 10, 15, and 20% concentrations of CCM showed better effect of 5% CGF in comparison to other concentrations(Chen, Jiao, et al, 2019;Jun et al, 2018).3.4 | In vivo effect of CGFSince the introduction of CGF by Sacco in 2006, many studies have evaluated the osteoinductive and regenerative properties of CGF in clinical studies as case series, animal studies, or clinical trials. In this regard, with excluding case series (without a control group), 18 studies on bone regeneration and six studies on regeneration of other tissues are listed inTables 4 and 5.…”