Concentrating Genomic Length DNA in a Microfabricated Array

Chen, Yu; Abrams, Ezra S.; Boles, T. Christian; Pedersen, Jonas Nyvold; Flyvbjerg, Henrik; Austin, Robert H.; Sturm, James C.

doi:10.1103/physrevlett.114.198303

Cited by 28 publications

(20 citation statements)

References 34 publications

(37 reference statements)

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“…[16], parasites [17], spores [18], bacteria [19], exosomes [20], and DNA [21,22]. Furthermore, recent progress enables the use of DLD pillar arrays as a platform for biomolecules detection including proteins and vesicles as seen in Fig.…”

Section: Introductionmentioning

confidence: 99%

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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HIGHLIGHTS• A well-organized and thorough discussion on the fundamental principles and recent progress in deterministic lateral displacement (DLD) is provided.• The most updated designs and applications of DLD techniques for particle separation and detection are reviewed.• The current limitations of DLD and its potential solutions for clinical and commercial applications are discussed.ABSTRACT The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics. In this field, microfluidic deterministic lateral displacement (DLD) holds a promise due to the ability of continuous separation of particles by size, shape, deformability, and electrical properties with high resolution. DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes. DLD techniques have been previously reviewed in 2014. Since then, the field has matured as several physics of DLD have been updated, new phenomena have been discovered, and various designs have been presented to achieve a higher separation performance and throughput. Furthermore, some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection. This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection. Furthermore, current challenges and potential solutions of DLD are also discussed. We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications. In particular, the rapid, low-cost, and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.

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Section: Introductionmentioning

confidence: 99%

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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“…173 Recently, Chen et al were able to apply a label-free passive deterministic lateral displacement technique to continuously concentrate genomic-length DNA by increasing the genome's shear modulus with 10% polyethylene glycol. 174 Kim et al employed a viscoelastic fluid flow to concentrate DNA towards the center of a microfluidic channel by both a flexibility-induced wall lift force and a fluid elastic force. 145 Protein.…”

Section: Biomolecule Separationmentioning

confidence: 99%

Advancements in microfluidics for nanoparticle separation

2017

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Nanoparticles have been widely implemented for healthcare and nanoscience industrial applications. Thus, efficient and effective nanoparticle separation methods are essential for advancement in these fields. However, current technologies for separation, such as ultracentrifugation, electrophoresis, filtration, chromatography, and selective precipitation, are not continuous and require multiple preparation steps and a minimum sample volume. Microfluidics has offered a relatively simple, low-cost, and continuous particle separation approach, and has been well-established for micron-sized particle sorting. Here, we review the recent advances in nanoparticle separation using microfluidic devices, focusing on its techniques, its advantages over conventional methods, and its potential applications, as well as foreseeable challenges in the separation of synthetic nanoparticles and biological molecules, especially DNA, proteins, viruses, and exosomes.

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“…Variations on this theme include pulsed field electrophoresis through arrays of micropillers 65 or nanoparticles (Fig. 2d) 66 , tilted Brownian ratcheting 67, 68 , and deterministic lateral displacement 69 . In general, these methods are limited to large DNA molecules (10’s to 100’s of kb) with limited demonstrated integration with single molecule detection.…”

Section: Assessment Modalitiesmentioning

confidence: 99%

“…However, single molecule analysis is useful for mechanistic and optimization studies. For example, analyzing the degree of DNA stretching under various solution conditions was used to improve throughput of deterministic lateral displacement separations with the addition of PEG 69 . Further improvements in DNA separation and sizing technologies on chip could allow for more efficient measurement and sorting prior to other downstream analyses.…”

Section: Assessment Modalitiesmentioning

confidence: 99%

Analysis of single nucleic acid molecules in micro- and nano-fluidics

2016

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Nucleic acid analysis has enhanced our understanding of biological processes and disease progression, elucidated the association of genetic variants and disease, and led to the design and implementation of new treatment strategies. These diverse applications require analysis of a variety of characteristics of nucleic acid molecules: size or length, detection or quantification of specific sequences, mapping of the general sequence structure, full sequence identification, analysis of epigenetic modifications, and observation of interactions between nucleic acids and other biomolecules. Strategies that can detect rare or transient species, characterize population distributions, and analyze small sample volumes enable the collection of richer data from biosamples. Platforms that integrate micro- and nano- fluidic operations with high sensitivity single molecule detection facilitate manipulation and detection of individual nucleic acid molecules. In this review, we will highlight important milestones and recent advances in single molecule nucleic acid analysis in micro- and nano- fluidic platforms. We focus on assessment modalities for single nucleic acid molecules and highlight the role of micro- and nano- structures and fluidic manipulation. We will also briefly discuss future directions and the current limitations and obstacles impeding even faster progress toward these goals.

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Concentrating Genomic Length DNA in a Microfabricated Array

Cited by 28 publications

References 34 publications

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

Advancements in microfluidics for nanoparticle separation

Analysis of single nucleic acid molecules in micro- and nano-fluidics

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