Concentration and desalting of Tetraselmis suecica crude extract by ultrafiltration

Rida, Hussein; Peydecastaing, Jérôme; Takache, Hosni; Ismail, Ali; Pontalier, Pierre-Yves

doi:10.1016/j.dwt.2024.100209

Cited by 3 publications

(1 citation statement)

References 54 publications

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“…These findings are consistent with those of Delran et al [20], who found that with a single pass at 400 These findings suggest that significant cell lysis occurred under these conditions, releasing 64.4% of the proteins. Similar results were obtained by Rida et al [39], who extracted 61.9 ± 3.9% of proteins from Tetraselmis suecica using 300 bars of pressure and two passes. Moreover, Tetraselmis suecica appears to have a weak cell wall.…”

Section: High-pressure Homogenization (Hph)supporting

confidence: 88%

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Rida,

Peydecastaing,

Takache

et al. 2024

Processes

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The optimization of cell disruption is a critical step in microalgal biorefineries. We used the same batch of Tetraselmis suecica culture to compare two mechanical cell disruption techniques, focusing on the extraction yield of water-soluble molecules. The conditions for high-pressure homogenization (HPH) studied were two passes at a moderate pressure of 300 bars. For ultrasound (US) treatment, we used an amplitude of 20% (equivalent to 100 W) for 25 min. These conditions were chosen on the basis of a preliminary screen of extraction conditions. HPH extracted proteins and pigments more efficiently than US, whereas US was superior for uronic acid extraction. Interestingly, the two methods had similar extraction yields for carbohydrates under the studied conditions. We also analyzed the kinetics of molecule release by considering the centrifugation time lag for HPH and applying a first-order kinetic model for US. HPH outperformed US in terms of the immediate extraction and release of molecules.

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Section: High-pressure Homogenization (Hph)supporting

confidence: 88%

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Rida,

Peydecastaing,

Takache

et al. 2024

Processes

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Coupling ultrasound and membrane filtration for the fractionation of Spirulina platensis sp. and the recovery of phycocyanin and pigment-free proteins

Obeid,

Rida,

Peydecastaing

et al. 2024

Biotechnol Lett

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The cyanobacterium Spirulina platensis was subjected to a fractionation process involving ultrasound-assisted extraction and membrane filtration to obtain a pure phycocyanin fraction and a clarified colorless protein fraction free of chlorophyll and carotenoids. The effects of pressure and power on total protein release were assessed. The retention of the extracted proteins was then assessed by ultrafiltration, with and without ammonium sulfate precipitation. Total protein recovery yields reached 97% in aqueous solution, at a low frequency (12 kHz), atmospheric pressure, and with an ultrasonic power of 200 Watts (W). Ammonium sulfate (25% w/v) precipitation was used to remove pigments and impurities from the crude protein extract. Finally, semi-frontal ultrafiltration resulted in high levels of C-phycocyanin recovery in the retentate: 95% and 91% with 10 and 100 kDa-cutoff membranes, respectively. However, the levels of total non-pigmented proteins in the permeate compartment did not exceed 67% with a 100 kDa-cutoff membrane. A fractionation process is proposed here for the valorization of two different protein fractions from Spirulina platensis.

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Anthroponics: Application and effects on growth of parsley, rhipsalis, coriander, and basil fed with urine fertiliser

Sohn,

El Saliby,

Merenda

et al. 2024

Desalination and Water Treatment

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Concentration and desalting of Tetraselmis suecica crude extract by ultrafiltration

Cited by 3 publications

References 54 publications

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Coupling ultrasound and membrane filtration for the fractionation of Spirulina platensis sp. and the recovery of phycocyanin and pigment-free proteins

Anthroponics: Application and effects on growth of parsley, rhipsalis, coriander, and basil fed with urine fertiliser

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