Concentration- and Time-dependent Effects of the Synthetic Estrogen, 17α-ethinylestradiol, on Reproductive Capabilities of the Zebrafish,<i>Danio rerio</i>

Schäfers, Christoph; Teigeler, Matthias; Wenzel, Andrea; Maack, Gerd; Fenske, Martina; Segner, Helmut

doi:10.1080/15287390701236470

Cited by 127 publications

(103 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…So, these two parameters do not appear to be relevant endpoints to detect the possible presence of organic pollutants in drinking waters at trace levels, or perhaps the substances that could have an effect are not present in the studied waters or, at least, are not present at harmful concentrations. In fact, studies have shown that chronic estrogen exposure of zebrafish to 17α-ethinylestradiol, which may be present in wastewaters and surface waters (Canonica et al, 2008), induced delayed onset of spawning and reduced fecundity and fertilization success at ng/L concentrations (Schäfers et al, 2007;Segner, 2009). …”

Section: Discussionmentioning

confidence: 99%

Zebrafish as a possible bioindicator of organic pollutants with effects on reproduction in drinking waters

Martínez-Sales

García-Ximénez

Espinós

2015

Journal of Environmental Sciences

View full text Add to dashboard Cite

Organic contaminants can be detected at low concentrations in drinking water, raising concerns for human health, particularly in reproduction. In this respect, we attempted to use the zebrafish as a bioindicator to detect the possible presence of these substances in drinking water, aiming to define the most relevant parameters to detect these substances, which particularly affect the development and reproduction of zebrafish. To this end, batches of 30 embryos with the chorion intact were cultured in drinking waters from different sources, throughout their full life-cycle up to 5 months, in 20 L tanks. Six replicates were performed in all water groups, with a total of 24 aquariums. Two generations (F0 and F1) were studied and the following parameters were tested: in the F0 generation, survival and abnormality rates evaluated at 5 dpf (days post fertilization) and at 5 mpf (months post fertilization), the onset of spawning and the fertility rate from 3 mpf to 5 mpf, and the sex ratio and underdeveloped specimens at 5 mpf. Furthermore, in the F0 offspring (F1), survival and abnormality rates were evaluated at 5 dpf and the hatching rate at 72 hpf. These results revealed that the hatching rate is the most sensitive parameter to distinguish different levels of effects between waters during the early life stages, whereas the rate of underdeveloped specimens is more suitable at later life stages. Regarding adult reproduction, fertility rate was the most sensitive parameter. The possible reversibility or accumulative nature of such effects will be studied in future work.

show abstract

Section: Discussionmentioning

confidence: 99%

Zebrafish as a possible bioindicator of organic pollutants with effects on reproduction in drinking waters

Martínez-Sales

García-Ximénez

Espinós

2015

Journal of Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…h For fathead minnow, P. promelas, the reported LOEC of 0.32 ng EE2/L (41) was considered a NOEC (see discussion in text). I The NOEC of 0.05 ng EE2/L for D. rerio reported by Segner et al (57) is excluded from the analysis because a subsequent study by this group using intermediate exposure concentrations resulted in NOECs of 0.31 and 0.36 ng/L for the F0 and F1 generations (42). j VTG from Hill and Janz (67).…”

Section: Nash Et Al 2005mentioning

confidence: 99%

Derivation of an Aquatic Predicted No-Effect Concentration for the Synthetic Hormone, 17α-Ethinyl Estradiol

Caldwell

Mastrocco

Hutchinson

et al. 2008

Environ. Sci. Technol.

236

138

View full text Add to dashboard Cite

17α-Ethinyl estradiol (EE2) is a synthetic estrogen widely used in combination with other steroid hormones in oral contraceptives and in the contraceptive patch. EE2 has been detected in sewage treatment plant effluents in the low nanogram -per-liter range and occasionally in surface waters in the U.S., U.K., Canada, Brazil, Germany, and elsewhere. The mode of action is receptor-mediated, and estrogen receptors exist in mammals and other vertebrates. A large number of studies on the effects of EE2 on aquatic organisms exist. One hundred English language studies published between 1994 and 2007, one as yet unpublished study, and findings published in conference proceedings (in German) were compared to published data quality criteria to identify the most relevant studies for deriving a predicted no-effect concentration (PNEC). Reproduction in fish was identified as the most sensitive end point in aquatic species. A species sensitivity distribution was constructed using no observed effect concentrations (NOECs) for reproductive effects from 39 papers in 26 species, resulting in a median hazardous concentration at which 5% of the species tested are affected (HC5,50) of 0.35 ng/L. After comparing this HC5,50 to all of the laboratory and field-derived toxicity information available for EE2, we recommend using 0.35 ng/L as the PNEC for EE2 in surface water. This PNEC is below 95% of the existing NOECs for effects on reproduction and is also below virtually all of the NOECs for vitellogenin induction in the key fish reproduction studies.

show abstract

“…The CF of HD fish was significantly reduced due to both reduced weight and length. Growth of fish has been reported to respond very sensitively to estrogen exposure (Ashfield et al 1998, Schäfers et al 2007). The underlying mechanisms of this growth inhibitory effect of estrogen exposure may be a crosstalk between the estrogen and the growth hormone (GH) / insulin-like growth factor (IGF) systems.…”

Section: Discussionmentioning

confidence: 99%

Long-term estrogen exposure of whitefish Coregonus lavaretus induces intersex but not Lake Thun-typical gonad malformations

Kipfer

Segner²,

Wenger³

et al. 2009

Dis. Aquat. Org.

View full text Add to dashboard Cite

A high prevalence of gonad morphological variations has been observed in whitefish Coregonus lavaretus from Lake Thun (Switzerland). To clarify the role of endocrine disruption as a possible cause of the gonad alterations, whitefish were reared in a long-term laboratory experiment under exposure to 17β-estradiol (E2). Fish were fed from first-feeding until 3 yr of age at a daily rate of 0 (control), 0.5 or 50 µg E2 kg -1 fish. E2 exposure resulted in a time-and concentration-dependent increase of prevalence and intensity of intersex gonads, i.e. gonads that macroscopically appeared as either testis or ovary but microscopically contained both male and female germ cells. Four types of intersex could be distinguished: Types 1 and 2 were composed of mainly male tissue, with Type 1 containing single oocytes and Type 2 displaying an ovary-like lamellar structure of the tissue. In Type 3, an increased percentage of the tissue was occupied by female germ cells, while in Type 4, the majority of the gonad tissue consisted of female germ cells. Chronic E2 exposure additionally resulted in a concentration-dependent shift of the sex ratio towards females, a reduced condition factor, retarded gonad growth together with delayed maturation of germ cells, and elevated levels of hepatic vitellogenin mRNA. However, Lake Thun-typical alterations of gonad morphology were not induced by chronic E2 exposure. The results provide evidence that estrogen-active compounds unlikely play a role in the etiology of gonad malformations in Lake Thun whitefish. KEY WORDS: Estrogen · Whitefish · Coregous lavaretus · Gonad morphology · Sex differentiation · Intersex · Vitellogenin · Endocrine disruption Resale or republication not permitted without written consent of the publisherDis Aquat Org 84: [43][44][45][46][47][48][49][50][51][52][53][54][55][56] 2009 drinking water for ~400 000 inhabitants of the area, a possible impact of environmental pollution is of particular public concern. In addition, whitefish is a frequently eaten fish and chemical contamination could therefore pose a risk to consumers. Relevant to human health and the health and development of the coregonid populations, it is thus important to clarify the causes of the gonad alterations.Variations in gonad morphology of fish have been reported in a number of studies worldwide (Kinnison et al. 2000, Blazer 2002, Mikaelian et al. 2002. In fact, the morphology and differentiation of the gonads of fish are known to be susceptible to a variety of environmental factors such as temperature (Patino et al. 1996, Baroiller et al. 1999, parasites (Wiklund et al. 1996, Jobling & Tyler 2003 and chemical substances (Kime 1995, Jobling et al. 1998. One environmental condition that has repeatedly been found to cause gonad alterations in fish is exposure to endocrine-disrupting compounds (EDCs) (Segner et al. 2003, Matozzo et al. 2007). Among the endocrine-active substances, chemicals that act as estrogen-receptor ligands and activate estrogen signaling pathways have received the mos...

show abstract

Concentration- and Time-dependent Effects of the Synthetic Estrogen, 17α-ethinylestradiol, on Reproductive Capabilities of the Zebrafish,Danio rerio

Cited by 127 publications

References 37 publications

Zebrafish as a possible bioindicator of organic pollutants with effects on reproduction in drinking waters

Zebrafish as a possible bioindicator of organic pollutants with effects on reproduction in drinking waters

Derivation of an Aquatic Predicted No-Effect Concentration for the Synthetic Hormone, 17α-Ethinyl Estradiol

Long-term estrogen exposure of whitefish Coregonus lavaretus induces intersex but not Lake Thun-typical gonad malformations

Contact Info

Product

Resources

About