Concentration dependence of fluorescence signal in a microfluidic fluorescence detector

Banerjee, Ansuman; Shuai, Yun; Dixit, Rahul; Papautsky, Ian; Klotzkin, D.

doi:10.1016/j.jlumin.2010.02.002

Cited by 40 publications

(41 citation statements)

References 16 publications

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“…A second bandpass filter centred on the fluorophore wavelength emission was used before the signal capture camera. The DB-OLED was used at 7.0 V with a total optical energy density of 85 mW/cm 2 (Banerjee et al, 2010). The fluorescence signal was acquired with a high sensitivity CCD camera, acquiring an image with a 12-bit digitalisation.…”

Section: Construction Of the Bio-sensor Sensitivity Curvementioning

confidence: 99%

OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples

Manzano

Cecchini

Fontanot

et al. 2015

Biosensors and Bioelectronics

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Section: Construction Of the Bio-sensor Sensitivity Curvementioning

confidence: 99%

OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples

Manzano

Cecchini

Fontanot

et al. 2015

Biosensors and Bioelectronics

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“…Banerjee and coworkers analyzed some of the parameters that affect the LOD [90] with the same setup they used previously [64] but with an Alq3-based green OLED, rather than a collimated metal halide lamp, as the excitation source. They repeated the detection of rhodamine 6G and demonstrated the effect of the depth of the microfluidic channel, the responsivity of the OPD, and the pump light power on the LOD.…”

Section: Signal To Noise Ratiomentioning

confidence: 99%

“…Measurements were conducted using 1 mM of Rhodamine 6G, OLED/OPD with 0.1 mA/W responsivity and 50 μm channel height with lock-in output, 5 V drive and 0.035 mA/W responsivity, and lock-in-input for 50 μm channel respectively. Reprinted from reference [90]; copyright 2010, with permission from Elsevier B.V.…”

Section: Signal To Noise Ratiomentioning

confidence: 99%

Organic Photodetectors in Analytical Applications

et al. 2015

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This review focuses on the utilization of organic photodetectors (OPDs) in optical analytical applications, highlighting examples of chemical and biological sensors and lab-on-a-chip spectrometers. The integration of OPDs with other organic optical sensor components, such as organic light emitting diode (OLED) excitation sources and thin organic sensing films, presents a step toward achieving compact, eventually disposable all-organic analytical devices. We discuss recent advances in developing and integrating OPDs for various applications as well as challenges faced in this area.

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“…The OLED has a flat surface, which makes it easy to integrate with microfluidic devices and is sufficiently flexible to permit it to be fabricated into the required size and shape using photolithography techniques. The OLED has been utilized as an excitation light source for a fluorescence detection system using microfluidics [37][38][39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, spectrometers [37], charge-coupled devices (CCDs) [38], photomultipliers (PMTs) [39], inorganic photodiodes (PDs) [40] and an organic photodiode (OPD) [41][42] can be used as the detector in such systems. Although the resolution of a spectrometer is sufficient, it is large and expensive and requires optics.…”

Section: Introductionmentioning

confidence: 99%

An integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection

Nakajima

Okuma

Morioka

et al. 2011

J of Separation Science

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A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm(2) was 13 μW (1.7 mW/cm(2)). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.

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Concentration dependence of fluorescence signal in a microfluidic fluorescence detector

Cited by 40 publications

References 16 publications

OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples

OLED-based DNA biochip for Campylobacter spp. detection in poultry meat samples

Organic Photodetectors in Analytical Applications

An integrated enzyme‐linked immunosorbent assay system with an organic light‐emitting diode and a charge‐coupled device for fluorescence detection

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