Concentration dependence of translational diffusion coefficients for globular proteins

Scott, David J.; Harding, Stephen E.; Winzor, Donald J.

doi:10.1039/c4an01060d

Cited by 30 publications

(24 citation statements)

References 59 publications

(131 reference statements)

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“…The anomalously high diffusion coefficient for degludec at low ionic strength, I , appears to be due to the effects of non-ideality (see Harding & Johnson 30 & Scott et al . 31 ) through unsuppressed charge repulsion effects. Upon the addition of salt and increase of I to approximate physiological conditions, these electrostatic interactions are suppressed, reducing the apparent diffusion coefficient.…”

Section: Discussionmentioning

confidence: 99%

Glargine and degludec: Solution behaviour of higher dose synthetic insulins

Adams

Alzahrani

Jiwani

et al. 2017

Sci Rep

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Single, double and triple doses of the synthetic insulins glargine and degludec currently used in patient therapy are characterised using macromolecular hydrodynamic techniques (dynamic light scattering and analytical ultracentrifugation) in an attempt to provide the basis for improved personalised insulin profiling in patients with diabetes. Using dynamic light scattering and sedimentation velocity in the analytical ultracentrifuge glargine was shown to be primarily dimeric under solvent conditions used in current formulations whereas degludec behaved as a dihexamer with evidence of further association of the hexamers (“multi-hexamerisation”). Further analysis by sedimentation equilibrium showed that degludec exhibited reversible interaction between mono- and-di-hexamer forms. Unlike glargine, degludec showed strong thermodynamic non-ideality, but this was suppressed by the addition of salt. With such large injectable doses of synthetic insulins remaining in the physiological system for extended periods of time, in some case 24–40 hours, double and triple dose insulins may impact adversely on personalised insulin profiling in patients with diabetes.

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Section: Discussionmentioning

confidence: 99%

Glargine and degludec: Solution behaviour of higher dose synthetic insulins

Adams

Alzahrani

Jiwani

et al. 2017

Sci Rep

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“…Scott et al [62] suggested an expression for the diffusion coefficient corresponding to Eq. (4.18) with D 0 replaced by D SE .…”

Section: Generalized Stokes-einstein Relationmentioning

confidence: 99%

Generalized Einstein relation for the mutual diffusion coefficient of a binary fluid mixture

Felderhof

2017

The Journal of Chemical Physics

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The method employed by Einstein to derive his famous relation between the diffusion coefficient and the friction coefficient of a Brownian particle is used to derive a generalized Einstein relation for the mutual diffusion coefficient of a binary fluid mixture. The expression is compared with the one derived by de Groot and Mazur from irreversible thermodynamics, and later by Batchelor for a Brownian suspension. A different result was derived by several other workers in irreversible thermodynamics. For a nearly incompressible solution the generalized Einstein relation agrees with the expression derived by de Groot and Mazur. The two expressions also agree to first order in solute density. For a Brownian suspension the result derived from the generalized Smoluchowski equation agrees with both expressions.

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“…Of additional concern is the assumed concentration-independence of the translational diffusion coefficient, an approximation that has been rendered difficult to verify by the essential demise of procedures for measuring D under the constraints of constant temperature and solvent chemical potential [25]. Although attention to these two aspects is clearly required in future studies attempting to take advantage of the suggested procedure, the present theoretical considerations have …”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 96%