Concentration‐dependent effect of bleaching agents on the immunolabelling of interleukin‐6, interleukin‐17 and CD5‐positive cells in the dental pulp

Benetti, Francine; Gomes‐Filho, João Eduardo; Ferreira, Leonardo L. G.; Sivieri-Araújo, Gustavo; Ervolino, Edílson; Briso, André Luiz Fraga; Cintra, Luciano Tavares Ângelo

doi:10.1111/iej.12891

Cited by 29 publications

(39 citation statements)

References 41 publications

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“…Additionally, other studies have reported that IL‐17 is related to inflammatory events after bleaching procedures (Benetti et al . , Ferreira et al . ).…”

Section: Review Of Identified Publicationsmentioning

confidence: 99%

The Presence and involvement of interleukin‐17 in apical periodontitis

2019

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Apical periodontitis (AP) is a chronic inflammatory disease characterized by periapical tissue inflammation and destruction of the associated alveolar bone. It is caused by microbial infections within the root canal and the resultant host immune responses in the periapical tissues. The proinflammatory cytokine interleukin (IL)‐17 has been shown to play an important role in many inflammatory diseases. There is increasing evidence of the presence of IL‐17 in AP, which might be associated with disease pathogenesis. Moreover, several animal studies indicate the potential role of IL‐17 in periapical inflammation and the resultant bone resorption in AP. This article reviews recent studies regarding the collective in vitro, in vivo and clinical evidence of the presence and involvement of IL‐17 in AP. A search related to IL‐17 in apical periodontitis was conducted on PubMed, EMBASE and Web of Science databases using keywords and controlled vocabulary. Two independent reviewers first screened titles and abstracts and then the full texts that were included. A total of 25 papers were identified, of the 25 included articles, 7 involved laboratory studies on cell cultures, 11 involved animal experimentations, and 7 were observational studies using human clinical samples. In conclusion, evidence for the presence of IL‐17 in AP from human and animal models is clear. However, there is relatively little information currently available that would highlight the specific role of IL‐17 in AP.

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“…Additionally, other studies have reported that IL‐17 is related to inflammatory events after bleaching procedures (Benetti et al . , Ferreira et al . ).…”

Section: Review Of Identified Publicationsmentioning

confidence: 99%

The Presence and involvement of interleukin‐17 in apical periodontitis

2019

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“…, , Benetti et al . , ). The animals were housed in a temperature‐controlled environment (22 °C ± 1 °C, 70% humidity and a 12‐h light–dark cycle) and received water and food ad libitum .…”

Section: Methodsmentioning

confidence: 99%

“…or submitted to immunohistochemical analysis by using an indirect immunoperoxidase technique (Benetti et al . , Ferreira et al . ) for osteocalcin, osteopontin and ROS.…”

Section: Methodsmentioning

confidence: 99%

“…These cells were present in the pulp even 30 days after the bleaching procedure (Benetti et al . ). The presence of apoptotic cells in the pulp tissue of rat molars was also observed after the use of higher concentrations of H 2 O 2 in the later periods, where pulp tissue was already organized (Benetti et al .…”

Section: Introductionmentioning

confidence: 97%

“…On the other hand, studies have verified the presence of CD5‐positive cells (Benetti et al . ), which may indicate the proliferation of thymocyte‐like or mature T‐lymphocyte cells and B cells (Lozano et al . , Brown & Lacey , de Wit et al .…”

Section: Introductionmentioning

confidence: 99%

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The presence of osteocalcin, osteopontin and reactive oxygen species‐positive cells in pulp tissue after dental bleaching

et al. 2018

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Aim To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS‐positive cells were counted. Paired t‐test and Wilcoxon signed‐rank test were used (P < 0.05). Results The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS‐positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions Post‐bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti‐ROS was involved in cellular defence against H2O2.

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