Concentration of foot-and-mouth disease virus by ultrafiltration

Adikane, H.V.; Nene, Sanjay; Kulkarni, Sneha A.; Baxi, Preeti; Khatpe, D.S.; Aphale, P.A.

doi:10.1016/s0376-7388(97)00056-2

Cited by 5 publications

(1 citation statement)

References 7 publications

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“…Inactivation was confirmed by the innocuity test in BHK-21 cell monolayer (OIE, 2012). Inactivated FMD virus suspension was concentrated to its 1/10th of original volume by ultrafiltration technique using 100 kDa hollow-fibre cartridge (Model: UFP-100-C-4MA; GE Healthcare) in AKTA Flux 6 (GE Healthcare) protein concentrator (Adikane et al, 1997). AKTA Pure 25 (GE Healthcare) fast performance liquid chromatography (FPLC) system was used to quantify the viral antigenic load in the concentrated virus suspension as previously described by Spitteler et al (2011).…”

Section: Monovalent Vaccine Preparationmentioning

confidence: 99%

Evidence of combined effect of amino acid substitutions within G‐H and B‐C loops of VP1 conferring serological heterogeneity in foot‐and‐mouth disease virus serotype A

Islam

Rahman

Amin

et al. 2020

Transbounding Emerging Dis

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Foot-and-mouth disease virus (FMDV) serotype A exhibits a higher degree of genetic and antigenic diversity resulting in frequent vaccine failure due to serological mismatch between the vaccine and heterologous strains. Currently, knowledge on the molecular basis of antigenic relationships among the FMDVs is limited; nevertheless, intratype antigenic variation due to mutation(s) is widely considered as the main hurdle to appropriate FMD vaccine development. Here, we studied genetic and antigenic variations of four FMDV serotype A isolates, BAN/GA/ Sa-197/2013 (BAN-197), BAN/CH/Sa-304/2016 (BAN-304), BAN/DH/Sa-307/2016 (BAN-307) and BAN/DH/Sa-310/2017 (BAN-310) circulating in Bangladesh during 2013-2017.Initially, antigenic relationships (r 1 -values) of the field isolates were evaluated by the two-dimensional microneutralization test (2D-MNT) using the hyperimmune antisera raised in cattle against the vaccine strain, BAN-304. Interesingly, the results showed protective serological cross-reactivity (r 1 -values > 0.4) between the vaccine strain and the field isolates, that substantially mismatched (r 1 = 0.129 ± 0.043) with the BAN-304. Although VP1-based phylogeny grouped all the isolates within the same sublineage C (a subgroup of VP3 Δ59 vari-

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Section: Monovalent Vaccine Preparationmentioning

confidence: 99%