Concentration of photosensitizers as a factor impacting on the results of fluorescent diagnostics

Maryakhina, Valeriya S.; Letuta, S. N.

doi:10.1088/1054-660x/24/3/035601

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…Concentration of bengal rose in all samples was 0.5 Á10 À4 M for exclusion of concentration e®ect. 4 Fluorescence spectra of photosensitizer in three media were measured on the°uorimetric setup described in our previous work.…”

Section: Introductionmentioning

confidence: 99%

Change of photosensitizer fluorescence at its diffusion in viscous liquid flow

Maryakhina

Gun’kov

2016

J. Innov. Opt. Health Sci.

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In this paper, the mathematical model of distribution of the injected compound in biological liquid°ow has been described. It is considered that biological liquid contains a few phases such as water, peptides and cells. The injected compound (for example, photosensitizer) can interact with peptides and cells. At the time, viscosity of the biological liquid depends on pathology present in organism. The obtained distribution of the compound connects on changes of its°uorescence spectra which are registered during°uorescent diagnostics of tumors. It is obtained that the curves do not have monotonic nature. There is a sharp curves decline in the¯rst few seconds after injection. Intensivity of curves rises after decreasing. It is especially pronounced for wavelength 590 nm and 580 nm (near the \transparency window" of biological tissues). Time of in-°e ction point shifts from 8.4 s to 6.9 s for longer wavelength. However, di®erence between curves is little for di®erent viscosity means of the biological liquid. Thus, additional pathology present in organism does not impact to the results of in vivo biomedical investigations.

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Section: Introductionmentioning

confidence: 99%

Change of photosensitizer fluorescence at its diffusion in viscous liquid flow

Maryakhina

Gun’kov

2016

J. Innov. Opt. Health Sci.

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“…Figure 1. Typical phosphorescence kinetics of xanthene dyes in different media: eosine in cells of wool of healthy (1) and cancer carrier mice with small (2) and large tumor (3), in glucose without cells (4)[5], rose bengal (5) and erythrosine (6) in collagenase[7].…”

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confidence: 99%

Nature of phosphorescence kinetics of xanthene dyes in biological media

Maryakhina¹

2016

Laser Phys.

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In the paper the experimental results on the nature of the phosphorescence of xanthene dyes in biological media are discussed. Phosphorescence is a monomolecular process and should have exponential type. However, the kinetics of the phosphorescence of xanthene dyes has two-exponential type in biological media. Analysis of data by experimental and theoretical methods showed that the second exponent connects on the phosphorescence of dye dimers. It can be used in biomedical investigation for dose selection of preparation delivery.

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Design and validation of a combined system for in situ measurement of photosensitizer fluorescence and tissue diffuse reflectance

Zhan,

Guo,

et al. 2024

Optics in Health Care and Biomedical Optics XIV

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Background: Quantitative measurement of photosensitizer during photodynamic therapy (PDT) is critical. One of the practical approaches is to measure photosensitizer's fluorescence, however, the influence of tissue optic properties needs to be considered in in situ measurement. Objective: To design and validate a combined system for dual detection of fluorescence and tissue diffuse reflectance. Materials and Method：For fluorescence detection, a 405 nm laser was used for excitation, a power meter for monitoring laser power and a fiber optic spectrometer for recording fluorescence. For diffuse reflectance measurement, a halogen lamp was used as light source and a fiber optic spectrometer for recording diffuse reflectance. Hemoporfin (HMME) was used as a model photosensitizer. Mice were used to evaluate the dual detection function of fluorescence and tissue diffuse reflectance after i.v. injection of HMME. Results: Solutions of different concentrations of HMME were used to test the intensity responses of the fluorescence detection system. A linear correlation was observed at HMME concentration lower than 10 μg/ml. Dual-band correction using corresponding diffuse reflectance data was used for the correction of original fluorescence spectrum. Diffuse reflectance corrected fluorescence data might truly reflect HMME concentration in the skin tissue. Conclusion: The preliminary evaluation suggests that the combined system can be used for quantitative measurement of photosensitizer fluorescence.

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Concentration of photosensitizers as a factor impacting on the results of fluorescent diagnostics

Cited by 3 publications

References 21 publications

Change of photosensitizer fluorescence at its diffusion in viscous liquid flow

Change of photosensitizer fluorescence at its diffusion in viscous liquid flow

Nature of phosphorescence kinetics of xanthene dyes in biological media

Design and validation of a combined system for in situ measurement of photosensitizer fluorescence and tissue diffuse reflectance

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