Concentration of plasma albumin in its accessible space in postmortem human dermis

Bert, Joel L.; Pearce, R. H.; Mathieson, J.

doi:10.1016/0026-2862(86)90055-5

Cited by 25 publications

(25 citation statements)

References 18 publications

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“…They applied a centrifugation technique to isolate interstitial fluid and assayed the distribution volumes for a range of endogenous proteins as well as radioactive probes (20,26,28). In this specialized tissue only 39% of the V i was available to albumin, in agreement with previous data from another collagen-rich tissue, namely, dermis (51). These experiments also suggested that the intrafibrillar water con- tained in collagen fibers was variable, a phenomenon that may affect fibril volume and organization and thus the overall exclusion value provided by such fibers (26).…”

Section: Interstitial Volume Exclusion In Tail Tendon and Rabbit Lungsupporting

confidence: 76%

“…Tumor tissue has at least an order of magnitude higher hydraulic conductivity than cornea (219, 447) that will facilitate fluid removal by centrifugation. Wiig et al (494) used the extracellular tracer 51 Cr-EDTA as a probe to show possible "contamination" of cellular fluid. Tumor cell fluid addition to the centrifuged volume should thus show up as a reduced 51 Cr-EDTA concentration in the centrifugate relative to plasma (FIGURE 10).…”

Section: Tissue Centrifugationmentioning

confidence: 99%

“…Wiig et al (494) used the extracellular tracer 51 Cr-EDTA as a probe to show possible "contamination" of cellular fluid. Tumor cell fluid addition to the centrifuged volume should thus show up as a reduced 51 Cr-EDTA concentration in the centrifugate relative to plasma (FIGURE 10). An observed ratio in peripheral tumor not significantly different from 1.0 for G Յ424 suggested no dilution of extracellular fluid, whereas the gradual reduc- tion of this ratio when exposing the tissue to higher Gforces indicated an increasing dilution probably due to extrusion of cellular fluid and/or cell damage.…”

Section: Tissue Centrifugationmentioning

confidence: 99%

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Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Wiig

Swartz²

2012

Physiological Reviews

577

574

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The interstitium describes the fluid, proteins, solutes, and the extracellular matrix (ECM) that comprise the cellular microenvironment in tissues. Its alterations are fundamental to changes in cell function in inflammation, pathogenesis, and cancer. Interstitial fluid (IF) is created by transcapillary filtration and cleared by lymphatic vessels. Herein we discuss the biophysical, biomechanical, and functional implications of IF in normal and pathological tissue states from both fluid balance and cell function perspectives. We also discuss analysis methods to access IF, which enables quantification of the cellular microenvironment; such methods have demonstrated, for example, that there can be dramatic gradients from tissue to plasma during inflammation and that tumor IF is hypoxic and acidic compared with subcutaneous IF and plasma. Accumulated recent data show that IF and its convection through the interstitium and delivery to the lymph nodes have many and diverse biological effects, including in ECM reorganization, cell migration, and capillary morphogenesis as well as in immunity and peripheral tolerance. This review integrates the biophysical, biomechanical, and biological aspects of interstitial and lymph fluid and its transport in tissue physiology, pathophysiology, and immune regulation.

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Section: Interstitial Volume Exclusion In Tail Tendon and Rabbit Lungsupporting

confidence: 76%

Section: Tissue Centrifugationmentioning

confidence: 99%

Section: Tissue Centrifugationmentioning

confidence: 99%

See 1 more Smart Citation

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Wiig

Swartz²

2012

Physiological Reviews

577

574

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“…The binding affinity of flurbiprofen for albumin is superior to that of ketoprofen, as mentioned above, and the binding ratios of the two drugs are 99.9% and 98.7%, respectively (Valko et al, 2003). It is notable that both the viable epidermis and dermis contain, on average, about 2% extravascular albumin, to which both flurbiprofen and ketoprofen can bind (Bert et al, 1986). The high lipophilicity and an effect of the superior protein-binding activity on the clearance mechanism are likely to be the major reasons why the skin concentration of flurbiprofen was higher than that of ketoprofen.…”

Section: Differences In the Time-course Changes In The Skin And Plasmmentioning

confidence: 80%

Evaluation of Percutaneous Drug Permeation Using a Lateral Sectioning Approach

Goi¹,

Morishita²,

Taniguchi³

et al. 2012

Readings in Advanced Pharmacokinetics - Theory, Methods and Applications

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“…Resulta interesante que los niveles de expresión de albúmina mostraron un perfil exactamente opuesto al de la hemoglobina, con mayor intensidad en la capa superficial de la cadera (comparada con la profunda) y en el depósito dorsal (comparado con el resto). Estas diferencias podrían estar influidas por el papel de la albúmina en el control de la presión osmótica intersticial y a una mayor proximidad de los vasos sanguíneos en estas localizaciones [10, 20]. …”

Section: Discussionunclassified

El proteoma del tejido adiposo subcutáneo muestra heterogeneidad anatómica

Martos‐Moreno

Sackmann-Sala

Berryman

et al. 2013

Anales de Pediatría

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Introducción El tejido adiposo blanco (TAB) subcutáneo (Sc) humano podría variar dependiendo de su localización anatómica, con diferencias en su perfil proteómico. Pacientes y métodos Se obtuvieron aspirados de TAB-Sc de seis mujeres con IMC >25 kg/m2, sometidas a liposucción. Dicho TAB-Sc se obtuvo de seis localizaciones anatómicas: abdominal superior e inferior, muslo, dorsal, flanco y cadera, analizándose su perfil proteómico mediante electroforesis bidimensional. En muslo y abdomen superior se compararon, además, las muestras obtenidas de las dos capas del TAB-Sc (profunda y superficial). Resultados Se detectaron 21 proteínas que mostraban una intensidad de expresión diferente entre las seis localizaciones anatómicas y 14 entre las capas superficial y profunda de una misma región. Entre las proteínas identificadas se incluyen: vimentina (proteína estructural); proteínas “heat-shock” (HSPs), superóxido-dismutasa, (estrés/chaperoninas); proteína fijadora de ácidos grasos 4 (FABP-4) y alfa-enolasa (metabolismo lipídico y de los hidratos de carbono, respectivamente) y ATP-sintetasa (producción de energía). Entre las regiones estudiadas, el TAB-Sc dorsal mostraba un perfil proteómico particular, con menor expresión de proteínas implicadas en la producción de energía y metabolismo (ATP-sintetasa, alfa-enolasa, HSPs y FABP-4) que el resto de regiones. Conclusiones Los niveles de expresión de diversas proteínas en el TAB-Sc humano no son homogéneos, difiriendo entre localizaciones anatómicas. Esto sugiere la existencia de diferencias funcionales en el TAB-Sc de acuerdo con su localización anatómica, lo que debe considerarse antes de asumir la extrapolación de los datos derivados del TAB-Sc de una determinada localización al de otras partes de la anatomía.

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Concentration of plasma albumin in its accessible space in postmortem human dermis

Cited by 25 publications

References 18 publications

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Interstitial Fluid and Lymph Formation and Transport: Physiological Regulation and Roles in Inflammation and Cancer

Evaluation of Percutaneous Drug Permeation Using a Lateral Sectioning Approach

El proteoma del tejido adiposo subcutáneo muestra heterogeneidad anatómica

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