Concentration of serum laminin and type IV collagen in liver diseases assayed by a sandwich enzyme-immunoassay using monoclonal antibodies

Yokoya, Yukihiro; Iwata, Kazushi; Muragaki, Yasuteru; Shiota, Chiyo; Morimoto, Yuuki; Inoue, Miyuki; Itoh, Hidekazu; Nishioka, Satoshi; Ooshima, Akira

doi:10.1016/0009-8981(92)90049-v

Cited by 14 publications

(11 citation statements)

References 16 publications

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“…Similar to our results, Friedman et al and others [11, 30, 31] found that serum levels of collagen IV and laminin were significantly higher in patients with hepatic disorders than in healthy controls. Although in our study, laminin was found to be the only marker that highly correlates with HAI (P < 0.01) in the viral group, Lu et al [32] reported that laminin has no diagnostic value either in inflammation or in fibrotic changes in patients with chronic liver disease.…”

Section: Discussionsupporting

confidence: 93%

Clinical Benefits of Biochemical Markers of Fibrosis in Egyptian Children With Chronic Liver Diseases

Abdel-Ghaffar¹

2011

Gastroenterol Res

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BackgroundThe need for repetition of liver biopsy, especially in assessing the degree of fibrosis and follow-up of treatment protocols, justifies an intensive search for non-invasive alternatives. We attempted to investigate the clinical usefulness of serum fibrogenesis markers in pediatric chronic liver diseases.MethodsWe measured serum levels of TGF-β1, collagen IV, laminin, MMP-2 and EGF-R, in 50 children with chronic liver disease (HBV, HCV and Bilharziasis) and 30 healthy controls, and determined their relationship to frequently used liver function tests and liver biopsy findings in patients.ResultsTGF-β1, collagen IV, laminin and MMP-2, but not EGF-R, were significantly higher in patients than in controls (P < 0.01). None of these markers correlated with the histological fibrosis stage, whereas laminin correlated with necroinflammatory activity (P < 0.01). TGF-β1, collagen IV, laminin and MMP-2 had the ability to discriminate patients with significant fibrosis, while only collagen IV and laminin were able to discriminate those with cirrhosis. Among these markers, collagen IV had the best predictive accuracy for significant fibrosis (AUROC 0.94; PPV 91.5%) and cirrhosis (AUROC 0.85; PPV 80%).ConclusionsIn conclusion, these markers may be useful in reducing but not replacing the need for liver biopsy in the monitoring of disease progression and treatment effectiveness and might be an inseparable part of assessment of chronic hepatopathies.

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Section: Discussionsupporting

confidence: 93%

Clinical Benefits of Biochemical Markers of Fibrosis in Egyptian Children With Chronic Liver Diseases

Abdel-Ghaffar¹

2011

Gastroenterol Res

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“…Type IV collagen is composed of three domains, that is, the major triple helix, the aminoterminal triple-helix (7s domain) and the carboxyterminal globular domains (NCl), and is organized in a network fashion (20). Serum levels of the 7s domain, the NC1 domain and the triple-helix are increased in chronic liver disease and correlate with the degree of the hepatic fibrosis (12, [21][22][23][24]. Besides these markers, serum TIMP-1 can serve as a marker of fibrosis (9-12).…”

Section: Discussionmentioning

confidence: 99%

Serum levels of tissue inhibitor of metalloproteinases‐2 and of precursor form of matrix metalloproteinase‐2 in patients with liver disease

Ebata

Fukuda

Nakano

et al. 1997

Liver

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Serum levels of tissue inhibitor of metalloproteinases-2 (TIMP2) and of precursor form of matrix metalloproteinase-2 (proMMP2) were determined in patients with chronic hepatitis and hepatocellular carcinoma by a one-step sandwich enzyme immunoassay. Serum levels of TIMP2 and proMMP2 were significantly higher in patients with chronic liver disease, than in normal controls. Serum levels of TIMP2 showed a weak negative correlation with the serum albumin level and prothrombin time (PT). Serum levels of proMMP2 in patients with chronic hepatitis were strongly correlated with those of type IV collagen and were negatively correlated with PT and serum albumin levels. Serum proMMP2 levels were also significantly correlated with histological stages. These data indicate that serum levels of proMMP2 might be useful in the fol-

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“…In an archetype basement membrane, the laminin lattice interweaves the lacework of type IV collagen, and nidogen (entactin) assumes the critical role of physically linking these two otherwise unconnected polymers [3]. The two predominant components in this architecture, type IV collagen and laminin, can be followed by radioimmunologic quantification of specific fragments, C-IV and P1, respectively, that are detectable in small amounts of serum and suitable as surrogate biomarkers for 'remote sensing' [4][5][6]. The C-IV epitope locates to a helical part of the type IV collagen molecule not involved in polymerization, the P1 epitope relates to the center of the branched laminin molecule.…”

Section: Introductionmentioning

confidence: 99%

Basement Membrane Biomarkers in Very Low Birth Weight Premature Infants

Aghai

Arevalo²,

Lumicao³

et al. 2002

Neonatology

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Basement membranes, critical for vital organs like the lungs, consist of two interwoven homopolymers, one assembled by type IV collagens and one by laminins. We hypothesized their serum antigens C-IV and P1, respectively, to be global measures for the maturity of these organs. In 39 very low birth weight premature neonates (means: gestational age, 25.8 weeks; birth weight, 779 g) requiring intensive care, we analyzed these biomarkers during the first two months post partum. Median C-IV and P1 exceeded adult levels by one order of magnitude. The individuals with the lowest first week C-IV values (mean: 667 ng/ml) required significantly longer neonatal intensive care unit stays than those with the highest values (mean: 2,467 ng/ml), on average 109 vs. 80 days (p = 0.008) irrespective of gestational age. Patients diagnosed with bronchopulmonary dysplasia (BPD) at 36 weeks postconceptional age, already in their first week of life displayed C-IV levels lower than in controls, suggesting a defect in pulmonary basement membrane remodeling. This is the first identification by a matrix biomarker of a BPD-antecedent state.

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Concentration of serum laminin and type IV collagen in liver diseases assayed by a sandwich enzyme-immunoassay using monoclonal antibodies

Cited by 14 publications

References 16 publications

Clinical Benefits of Biochemical Markers of Fibrosis in Egyptian Children With Chronic Liver Diseases

Clinical Benefits of Biochemical Markers of Fibrosis in Egyptian Children With Chronic Liver Diseases

Serum levels of tissue inhibitor of metalloproteinases‐2 and of precursor form of matrix metalloproteinase‐2 in patients with liver disease

Basement Membrane Biomarkers in Very Low Birth Weight Premature Infants

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