Concentration of Transforming Growth Factor-beta 1 in Chronic Periapical Lesions / Ispitivanje koncentracije transformišućeg faktora rasta beta 1 u hroničnim periapeksnim lezijama

Popović, Jelena; Cvetković, Tatjana; Džopalić, Tanja; Mitić, Aleksandar; Nikolić, Marija; Barac, Radomir

doi:10.1515/afmnai-2015-0004

Cited by 1 publication

(1 citation statement)

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“…Although TGF-β stimulates initially the inflammatory responses by attracting lymphocytes and monocytes to the inflammatory site, it suppresses the proliferation of these cells as well as the production of different proinflammatory cytokines (19). TGF-β is also expressed in PLs (16,20), irrespective of whether PLs are asymptomatic or symptomatic. It can be postulated that this cytokine is important for down-regulation of inflammatory and immune responses within PLs and for the repair of periapical bone loss.…”

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Marković¹,

Tomić²,

Djokić³

et al. 2015

Acta Facultatis Medicae Naissensis

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The pathophysiology of periapical lesions (PLs) is under control of pro-inflammatory and anti-inflammatory (mainly immunoregulatory) cytokines. We have recently established mesenchymal stem cells (MSCs) from PLs and showed their suppressive effects on the production of proinflammatory cytokines from PLs inflammatory cells (ICs). In this work we studied the production of interleukin (IL)-10, IL-27 and transforming growth factor (TGF)-β, by PL-ICs in direct or indirect contacts with PL-MSCs. PL-ICs, which were isolated from four different asymptomatic PLs, predominantly composed of lymphocytes, followed by neutrophil granulocytes, macrophages and plasma cells. PLMSCs, expressing typical MSC markers, were co-cultivated with PL-ICs at 1:10 ratio, either in direct contact or in a transwell-system, for 24 hours. The levels of cytokines in cell-culture supernatants were tested by ELISA. The results showed that PL-MSCs up-regulated the production of all three immunoregulatory cytokines by PL-ICs. PL-MSCs stimulated the production of IL-10 and IL-27 via soluble factors, whereas the up-regulation of TGF-β required direct cell-to-cell contacts. In conclusion, our results showed for the first time the involvement of PL-MSCs in restriction of inflammation in PLs by up-regulation of immunoregulatory cytokines.

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Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%