Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows

Kitano, Nana; Isobe, Naoki; Noda, Jun; Takahashi, Toshihiko

doi:10.1111/asj.13372

Cited by 5 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of LAP in milk infected with S. aureus, Streptococcus bovis, Streptococcus dysgalactiae, and E. coli was found to be significantly higher than that in uninfected milk (Kawai et al 2013). Kitano et al (2020) states that the concentration patterns of LAP secreted in milk from the udders of healthy lactating cows follow the baseline data. These distinct concentration patterns might indicate various protective responses.…”

Section: Discussionmentioning

confidence: 70%

Polish Journal of Veterinary Sciences

Šerstņova¹,

Pilmane²,

Vitenberga-Verza³

et al. 2022

View full text Add to dashboard Cite

Great economic losses to the dairy industry are associated with bovine mastitis, which results in poor milk quality and high treatment costs. Anti-inflammatory proteins play an important role in the suppression of the immune response against invading pathogenic microorganisms and are therefore being studied for possible use in the early diagnosis of mastitis. In our study, we used milk samples from 15 cows of Holstein Friesian breed with different health status (5 healthy, 5 subclinical, and 5 clinical animals), and tested them using immunohistochemical (IHC) analysis to evaluate the presence of IL-2, IL-10, TGF-β1, βDEF-2, DEF-3, and Cathelicidin LL37 proteins. The calculation of positively and negatively stained cells for each biomarker was performed using the semiquantitative counting method. We found the presence of all factors with the exception of Cathelicidin LL37, which was almost absent in milk samples of all animal groups. The significant decrease of IL-10, β-def2, and β-def3 expression levels within the 3 days of sampling, found in the milk of animals with sub-and clinical mastitis, indicates the loss of antiinflammatory protection of the affected cow's udder. In contrast, the stable increase of IL-2 and TGF-β1 positive cells observed in the milk of mastitis-affected cows, and the similar expression of these factors in the milk of healthy animals, indicate the possible lack of involvement of these cytokines at an early stage of udder inflammation.

show abstract

Section: Discussionmentioning

confidence: 70%

Polish Journal of Veterinary Sciences

Šerstņova¹,

Pilmane²,

Vitenberga-Verza³

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It has been reported that inflammation-related factors, such as intramammary lipopolysaccharide administration [ 52 ] and intramammary infection of E. coli [ 41 ], increase the S100A7 concentration. It has also been pointed out that even in healthy milk, the S100A7 concentration is lowest in the first pre-squeezed milk, then it gradually increases [ 24 ], and it is also affected by the season [ 48 ]. From these studies, it was considered that the milk samples investigated in this study were not from mammary glands in an inflammatory state, and that known factors that influence the S100A7 concentration were sufficiently excluded.…”

Section: Discussionmentioning

confidence: 99%

Regression tree analysis of the relationship between the concentrations of antimicrobial components and the microbiota of normal milk from dairy cows

Shinozuka

Suzuki

Kaneko

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to determine the concentrations of antimicrobial components (immunoglobulin A (IgA), lactoferrin (LF), lingual antimicrobial peptide (LAP), and S100A7) in normal milk and their relation to host factors (Age, somatic cell count (SCC), days in milk, richness, and alpha diversity of the milk microbiota) in dairy cows using multivariate regression tree analyses, and to clarify how the milk microbiota is related to the obtained results. Thirty normal milk samples were collected from a commercial dairy farm in June 2020. The thresholds that predicted the concentration of each antimicrobial component in milk were obtained by regression tree analysis, and the beta-diversity of the milk microbiota composition between groups divided according to each threshold was compared by an analysis of similarities test. The IgA and LF concentrations were mainly predicted by the SCC (177,500 and 70,000 cells/ml, respectively), and the LAP and S100A7 concentrations were predicted by Age (29.667 and 40.3 months, respectively). No relationship was observed between the concentration of IgA, LAP, or S100A7 and the milk microbiota composition between the groups divided by the threshold for prediction, but the milk microbiota composition was significantly different between the groups divided by the threshold for predicting the LF concentration.Our results indicated that the LF concentration in normal milk may be associated with the milk microbiota composition.

show abstract

“…S100A7, an AMP with a strong antimicrobial activity [ 16 , 17 ], which is first identified in the epithelial cells of human psoriasis skin [ 18 , 19 ], has been proved to play a critical role in IRR against the invasion and infection of pathogenic microorganisms. S100A7 can be detected in bovine milk [ 20 , 21 ] and serum [ 22 ]. In a bovine udder, the expression of S100A7 is common in teat skin and the streak canal [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…S100A7 can be detected in bovine milk [ 20 , 21 ] and serum [ 22 ]. In a bovine udder, the expression of S100A7 is common in teat skin and the streak canal [ 20 , 21 ]. But the expression of S100A7 was limited to bacteria-infected teat cisterns rather than the epithelial cells of the alveolus [ 17 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells

Yan

Niu

et al. 2022

Animals

View full text Add to dashboard Cite

S100A7 has received extensive attention in the prevention and treatment of mastitis across a broad spectrum, yet there is a little information about its mechanism, especially in the immunomodulatory effects of estrogen. In the present study, based on the milk bacteriological culture (BC) of 30 dairy goats, the concentration of both estrogen and S100A7 in the BC-positive samples was not significantly different than in the BC-negative samples; the estrogen abundance in subclinical and clinical mastitis samples also showed only a limited difference; compared with healthy samples, the S100A7 abundance in subclinical mastitis samples differed little, while it was significantly decreased in clinical mastitis samples. Moreover, the relationship between estrogen and S100A7 was positive, and the regression equation was y = 0.3206x + 23.459. The goat mammary epithelial cells (gMECs) were isolated and treated with 1, 10, 100 nM E2 and/or 5 μg/mL lipopolysaccharide (LPS), respectively, for 6 h. Compared with control samples, 5 μg/mL LPS, 10 nM E2 and 100 nM E2 markedly induced S100A7 expression and secretion. More than separated treatment, the cooperation of LPS and E2 also significantly increased S100A7 expression, rather than S100A7 secretion. The p-ERK was up-regulated markedly with 100 nM E2 treatment, while the expression of p-JNK, p-p38 and p-Akt had little effect. The G protein-coupled estrogen receptor 1(GPER1) agonist G1 markedly induced S100A7 expression and secretion in gMECs, and the estrogen nuclear receptor antagonist ICI and GPER1 antagonist G15 significantly repressed this process. In conclusion, E2 binds to nuclear and membrane receptors to regulate the expression and secretion of S100A7 via the ERK1/2-signaling pathway in gMECs.

show abstract

Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows

Cited by 5 publications

References 18 publications

Polish Journal of Veterinary Sciences

Polish Journal of Veterinary Sciences

Regression tree analysis of the relationship between the concentrations of antimicrobial components and the microbiota of normal milk from dairy cows

Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells

Contact Info

Product

Resources

About