Concentration profile of tobramycin in exhaled breath condensate after inhalation of a single dose: A pilot study

Khoubnasabjafari, Maryam; Azarbayjani, Anahita Fathi; Rahimpour, Elaheh; Jouyban-Gharamaleki, Vahid; Kim, Hannah Yejin; Alffenaar, Johannes; Chan, Hak‐Kim; Jouyban, Abolghasem

doi:10.1016/j.jddst.2021.102394

Cited by 7 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wide variations of drug concentrations in EBC were observed in many published works. The mean values and standard deviations for a concentration-time profile of tobramycin in EBC were reported in earlier work ( 12 ). The variations of tobramycin in EBC were also reported in another work ( 45 ).…”

Section: Discussionmentioning

confidence: 82%

“…In the following study, the applicability of the hypothesis was tested on the tobramycin profile in EBC. Although the results of this small-size pilot study were promising, we had two main limitations; i.e., only one tobramycin inhaled formulation was available, and we used an analytical method with low sensitivity and selectivity, which resulted in wide variations of EBC concentration profiles of six healthy volunteers ( 12 ). In another interesting work, Sadiq et al ( 13 ) investigated the lung pharmacokinetics of several inhaled and orally administered drugs including salbutamol.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new method for investigating bioequivalence of inhaled formulations: A pilot study on salbutamol

Rezaei¹,

Khoubnasabjafari

Jouyban-Gharamaleki

et al. 2023

J. pharm. pharm. sci.

Self Cite

View full text Add to dashboard Cite

Purpose: An efficient, cost-effective and non-invasive test is required to overcome the challenges faced in the process of bioequivalence (BE) studies of various orally inhaled drug formulations. Two different types of pressurized meter dose inhalers (MDI-1 and MDI-2) were used in this study to test the practical applicability of a previously proposed hypothesis on the BE of inhaled salbutamol formulations.Methods: Salbutamol concentration profiles of the exhaled breath condensate (EBC) samples collected from volunteers receiving two inhaled formulations were compared employing BE criteria. In addition, the aerodynamic particle size distribution of the inhalers was determined by employing next generation impactor. Salbutamol concentrations in the samples were determined using liquid and gas chromatographic methods.Results: The MDI-1 inhaler induced slightly higher EBC concentrations of salbutamol when compared with MDI-2. The geometric MDI-2/MDI-1 mean ratios (confidence intervals) were 0.937 (0.721–1.22) for maximum concentration and 0.841 (0.592–1.20) for area under the EBC-time profile, indicating a lack of BE between the two formulations. In agreement with the in vivo data, the in vitro data indicated that the fine particle dose (FPD) of MDI-1 was slightly higher than that for the MDI-2 formulation. However, the FPD differences between the two formulations were not statistically significant.Conclusion: EBC data of the present work may be considered as a reliable source for assessment of the BE studies of orally inhaled drug formulations. However, more detailed investigations employing larger sample sizes and more formulations are required to provide more evidence for the proposed method of BE assay.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

A new method for investigating bioequivalence of inhaled formulations: A pilot study on salbutamol

Rezaei¹,

Khoubnasabjafari

Jouyban-Gharamaleki

et al. 2023

J. pharm. pharm. sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Likewise, Khoubnasabjafari et al conducted a pilot study on healthy volunteers who had received an inhaled monodose of tobramycin. They successfully detected tobramycin in all the EBC samples collected from an expiratory reservoir [35].…”

Section: Discussionmentioning

confidence: 99%

Beta-Lactam Antibiotics Can Be Measured in the Exhaled Breath Condensate in Mechanically Ventilated Patients: A Pilot Study

Escalona

Soto

Oviedo

et al. 2023

JPM

View full text Add to dashboard Cite

Different techniques have been proposed to measure antibiotic levels within the lung parenchyma; however, their use is limited because they are invasive and associated with adverse effects. We explore whether beta-lactam antibiotics could be measured in exhaled breath condensate collected from heat and moisture exchange filters (HMEFs) and correlated with the concentration of antibiotics measured from bronchoalveolar lavage (BAL). We designed an observational study in patients undergoing mechanical ventilation, which required a BAL to confirm or discard the diagnosis of pneumonia. We measured and correlated the concentration of beta-lactam antibiotics in plasma, epithelial lining fluid (ELF), and exhaled breath condensate collected from HMEFs. We studied 12 patients, and we detected the presence of antibiotics in plasma, ELF, and HMEFs from every patient studied. The concentrations of antibiotics were very heterogeneous over the population studied. The mean antibiotic concentration was 293.5 (715) ng/mL in plasma, 12.3 (31) ng/mL in ELF, and 0.5 (0.9) ng/mL in HMEF. We found no significant correlation between the concentration of antibiotics in plasma and ELF (R2 = 0.02, p = 0.64), between plasma and HMEF (R2 = 0.02, p = 0.63), or between ELF and HMEF (R2 = 0.02, p = 0.66). We conclude that beta-lactam antibiotics can be detected and measured from the exhaled breath condensate accumulated in the HMEF from mechanically ventilated patients. However, no correlations were observed between the antibiotic concentrations in HMEF with either plasma or ELF.

show abstract

“…In a similar study, UV–vis spectroscopy has been utilized for tobramycin detection from breath samples of healthy subjects inhaling antibiotics for a certain amount of time. [ 32 ] However, also in this study, no investigation has been performed to correlate antibiotic levels in plasma and EBC. Some attempts have been made to quantify different types of antibiotics in EBC using chromatographic methods.…”

Section: Introductionmentioning

confidence: 98%

Biosensor‐Enabled Multiplexed On‐Site Therapeutic Drug Monitoring of Antibiotics

et al. 2021

View full text Add to dashboard Cite

Personalized antibiotherapy ensures that the antibiotic concentration remains in the optimal therapeutic window to maximize efficacy, minimize side effects, and avoid the emergence of drug resistance due to insufficient dosing. However, such individualized schemes need frequent sampling to tailor the blood antibiotic concentrations. To optimally integrate therapeutic drug monitoring (TDM) into the clinical workflow, antibiotic levels can either be measured in blood using point‐of‐care testing (POCT), or can rely on noninvasive sampling. Here, a versatile biosensor with an antibody‐free assay for on‐site TDM is presented. The platform is evaluated with an animal study, where antibiotic concentrations are quantified in different matrices including whole blood, plasma, urine, saliva, and exhaled breath condensate (EBC). The clearance and the temporal evaluation of antibiotic levels in EBC and plasma are demonstrated. Influence of matrix effects on measured drug concentrations is determined by comparing the plasma levels with those in noninvasive samples. The system's potential for blood‐based POCT is further illustrated by tracking ß‑lactam concentrations in untreated blood samples. Finally, multiplexing capabilities are explored successfully for multianalyte/sample analysis. By enabling a rapid, low‐cost, sample‐independent, and multiplexed on‐site TDM, this system can shift the paradigm of “one‑size‐fits‐all” strategy.

show abstract

Concentration profile of tobramycin in exhaled breath condensate after inhalation of a single dose: A pilot study

Cited by 7 publications

References 19 publications

A new method for investigating bioequivalence of inhaled formulations: A pilot study on salbutamol

A new method for investigating bioequivalence of inhaled formulations: A pilot study on salbutamol

Beta-Lactam Antibiotics Can Be Measured in the Exhaled Breath Condensate in Mechanically Ventilated Patients: A Pilot Study

Biosensor‐Enabled Multiplexed On‐Site Therapeutic Drug Monitoring of Antibiotics

Contact Info

Product

Resources

About