Concentration, quantitation, and identification of hemopoietic stem cells.

Thymus-dependent lymphocytes in human bone marrow.

“…These cells and their progenitor, the pluripotent hematopoietic stem cell, are morphologically similar to lymphocytes (15,16). In murine systems, hematopoietic stem cells have been separated from lymphocytes by density centrifugation (7,17).…”

A Computational Simulation Technology for Specific Counting of Perinatal and Postnatal Human Tissue Stem Cells for Transplantation Medicine

“…In particular, these approaches were applied to several challenging problems in stem cell biology that were difficult to interrogate by observational or experimental approaches. These more challenging problems included identifying and quantifying tissue stem cells in vivo, discovering the mathematical form of their competing self-renewal and production of committed progenitor cells, and investigating how tissue stem cells and their subtended cellular systems respond to physiological factors and exogenous agents like environmental toxicants and drugs [38][39][40][41][42][43][44][45][46]. Beyond relevance to important health-related processes in the body (e.g., normal growth and maturation, diseases like cancer, and aging), knowledge of such tissue stem cell properties is crucial to in vitro processes, too, like tissue engineering, stem cell manufacturing, stem cell transplantation medicine, and drug discovery.…”

“…The most actively pursued tissue stem cell modelling has focused on the identity and selfrenewal properties of hematopoietic stem cells [36,[38][39][40][41][42] or gastrointestinal tract stem cells, including primarily small intestinal epithelial stem cells [12,[47][48][49] and, to a lesser extent, colonic epithelial stem cells [43]. Recent modelling reports [40][41][42][43][44][45][46][47][48][49][50][51][52] focus on the still unsettled question whether tissue stem cells achieve their balance of self-renewal versus tissue cell renewal by stochastic or deterministic division and differentiation programs [41,[53][54][55].…”

Annotation on Stem Cells and Differentiation Fields

“…By 1967 there were some data on the sue and general morphology of the presumptive normal hemopoietic stem cell (NHSC) in mice [18]. Unfortunately, the techniques used in purifying such cells from normal tissues were very laborious and only partially successful.…”