Concentrations of 5-Hydroxytryptamine in Rat Brain and Pineal after Adrenalectomy and Cortisol Administration

McKennee, C.T.; Timiras, Paola S.; Quay, W.B.

doi:10.1159/000121672

Cited by 18 publications

(4 citation statements)

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“…The presented data clearly show that the action of the glucocorticoid is time-dependent. All changes induced by the dose used are diminished after 2 h and the most remarkable changes can be seen within 1 h. This would certainly explain some of the discrepancies between different authors, namely that at a certain time after corticoid treatment no change in brain serotonin content could be seen [McKennee et al, 1966;Shah et al, 1968].…”

Section: Discussionmentioning

confidence: 97%

Effect of Adrenocortical Hormones on Activity of the Serotoninergic System in Limbic Structures in Rats

Telegdy

Vermes²

1975

Neuroendocrinology

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A single dose of corticosterone (1 mg/kg b.w. i.p.) increased the 5-hydroxytryptamine (5-HT) content in the mesencephalon, amygdala and hypothalamus. The maximum was observed at 15 min following administration. The 5-HT level returned to normal between 60 and 180 min. The 5-hydroxyindoleacetic acid (5-HIAA) content decreased in the mesencephalon and hypothalamus at 15 min, and then increased to the maximum at 30 min in the mesencephalon and at 45 min in the hypothalamus and amygdala. There were no changes in the septum and hippocampus either in 5-HT or in 5-HIAA content following corticosterone administration. Desoxycorticosterone administration (1 mg/rat i.p.) was ineffective. Tritiated 5-HT uptake did not change in the hypothalamus and mesencephalon tissue in vitro after corticosterone or desoxycorticosterone treatment. Adrenalectomy caused a decrease in the 5-HT content in all brain areas studies. 5-HIAA decreased only in the hypothalamus. Corticosterone administration normalized the 5-HT and 5-HIAA content in the hypothalamus and mesencephalon in adrenalectomized rats. Tritiated 5-HT uptake was lower in the hypothalamus and mesencephalon in adrenalectomized rats. Corticosterone administration increased the activity back to normal in the hypothalamus, however desoxycorticosterone was ineffective. The data suggest that the plasma corticosterone level plays a role in the regulation of the activity of the serotoninergic system in certain limbic brain structures.

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Section: Discussionmentioning

confidence: 97%

Effect of Adrenocortical Hormones on Activity of the Serotoninergic System in Limbic Structures in Rats

Telegdy

Vermes²

1975

Neuroendocrinology

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“…Several studies have shown that turnover of 5-HT decreases within several hours after ADX due to de creases in the activity of the synthetic enzyme tryptophan hydroxylase [12,13]. However, many researchers find no significant effects of ADX on levels or turnover of 5-HT following the initial few hours after surgeir [14,16]. In deed, the primary effect of ADX on the metabolism of 5-HT may be to prevent the permissive role of CORT in the stress induction of tryptophan hydroxylase activity [16][17][18].…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic Analyses of the Effects of Adrenalectomy and Corticosterone on 5-HT<sub>1A</sub> and 5-HT<sub>1B</sub> Receptors in the Dorsal Hippocampus and Cortex of the Rat

Mendelson

McEwen²

1992

Neuroendocrinology

174

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Quantitative autoradiography was used to evaluate the effects of adrenalectomy (ADX) and corticosterone (CORT) on binding at 5-HT_1A and 5-HT_1Breceptors in the dorsal hippocampus and cortex of the rat. ADX increased binding of [³H]8-hydroxy-2-(di-n-propylamino)tetralin at 5-HT_1A receptors in the oriens and lacunosum moleculare layers of CA2 and CA3, in the lacuno-sum moleculare layer of CA4 region, and in the dentate gyms. In restraint-stressed ADX rats, binding was increased only in the oriens and lacunosum moleculare layers of CA2. Restoration of baseline levels of CORT reversed the effects of ADX on 5-HT_1A receptors in the hippocampus, while high levels of CORT decreased binding at 5-HT_1A receptors in the dentate gyms. No treatment affected binding at 5-HT_1A receptors in the CAl region of the hippocampus or in the cortex. ADX increased binding of [¹²⁵I]iodocyanopindolol at 5-HT_1B receptors in the infrapyramidal dentate, but this effect was not observed in ADX rats that were restrained. CORT treatment in both ADX and SHAM (adrenally intact) rats resulted in binding at 5-HT_1B receptors that was lower than that in untreated ADX and SHAM rats in the infrapyramidal dentate, and lower than that in ADX rats in the suprapyramidal dentate and CA4. In ADX and SHAM rats, CORT also reduced binding at 5-HT_1B receptors in area 2 of the cortex. It is suggested that decreases in binding at 5-HTA and 5-HT_1B/1D receptors resulting from chronic exposure to high levels of CORT may also occur in animals that fail to adapt to chronic severe stress. Such changes in binding may play important roles in the etiology of depression.

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“…Following administration of corticosteroids, brain serotonin content was decreased (11-13), unchanged (14,15), or increased (11,16). According to Telegdy and Vermis (17), a single dose of corticosterone increased the serotonin content in the mesen cephalon, amygdala and hypothalamus.…”

Section: Resultsmentioning

confidence: 99%

Effect of neonatal hydrocortisone treatment on brain monoamines in developing rats.

Kurosawa¹,

Kageyama²,

JOHN³

et al. 1980

Jpn.J.Pharmacol

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Abstract-In our previous study, neonatal treatment with hydrocortisone was shown to produce a marked retardation of pituitary-adrenocortical development in infant rats. The present investigation was an attempt to determine whether or not the retarded activity is caused by functional changes in brain monoamine systems. In rats treated with hydrocortisone (0.5 mg/rat, s.c.) on the 2nd day of life, the development of whole brain was suppressed significantly. However, norepinephrine, dopamine and serotonin contents in the brain were higher in these rats than in controls. These changes of mono amine contents were apparent in the hypothalamus, diencephalon and pons-medulla oblongata.Our data suggest that monoaminergic nervous systems are potentiated with hydrocortisone in these brain regions, although the results do not necessarily explain the retarded hypothalamo-pituitary function.Neonatal treatment of rats with several corticosteroids caused a marked atrophy of the adrenal gland and the thymus, these producing the wasting syndrome (1). In the treated animals, circadian rhythm of plasma corticosterone appeared at 4 weeks of age, but the plasma levels of corticosterone were significantly lower than those of controls until 6 weeks (2). Since the secretion of ACTH is likely to be affected by brain monoamines (3), the lowered plasma corticosterone levels in neonatally hydrocortisone-treated rats might be related to changes in monoamine contents in the brain. In this regard, Ulrich et al. (4) reported that both norepinephrine and serotonin in the medial hypothalamus were elevated, but dopamine levels did not increase in those rats at 30 days of age. However, these workers determined the content in the hypothalamus at one age only. More recently, Nyakas (5) found an increased norepinephrine content in the hindbrain of 4 month old rats given corticosterone on the 3rd to 5th postnatal days. The present study was undertaken to observe serial changes in monoamine contents from 1 to 7 weeks of age in the brain of rats given hydrocortisone neonatally. Our results are related to the ontogenic pattern of brain monoamines, as affected by hydrocortisone treatment. MATERIALS AND METHODSMale and female Wistar rats were housed at a constant temperature of 25±2'C under controlled lighting (fluorescent illumination from 07:00 to 19:00). Rat biscuits (Oriental Yeast Co.) and water were given ad lib.Newborn rats were given 0.5 mg hydrocortisone acetate (Merck) in 0.05 ml solvent s.c., on the 2nd day of life. The solvent consisted of 0.4 % polysorbate-80, 0.5 % carboxy methylcellulose and 0.5% benzylalcohol in 0.9% NaCI. Control rats were given the solvent only. About three-fourths of the litters were treated with hydrocortisone and the remaining served as controls. The rats were left with their mothers until weaning at 21 days of life.Almost 1,000 newborn rats were used, but more than 50 % of the infant rats given hydro cortisone died within 10 days.The animals were decapitated between 13:00 and 14:00 hr. The whole brain was quickly remov...

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Concentrations of 5-Hydroxytryptamine in Rat Brain and Pineal after Adrenalectomy and Cortisol Administration

Cited by 18 publications

References 1 publication

Effect of Adrenocortical Hormones on Activity of the Serotoninergic System in Limbic Structures in Rats

Effect of Adrenocortical Hormones on Activity of the Serotoninergic System in Limbic Structures in Rats

Autoradiographic Analyses of the Effects of Adrenalectomy and Corticosterone on 5-HT<sub>1A</sub> and 5-HT<sub>1B</sub> Receptors in the Dorsal Hippocampus and Cortex of the Rat

Effect of neonatal hydrocortisone treatment on brain monoamines in developing rats.

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