Concentrations of energy substrates in oviduct fluid in unilaterally ovariectomised pigs

Nichol, R. C.; Hunter, R. H. F.; Partridge, R.J.; Leese, Henry J.; Cooke, Gerard M.

doi:10.1016/s0034-5288(98)90154-0

Cited by 12 publications

(6 citation statements)

References 16 publications

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“…The most important difference in mitochondrial membrane potential between developmentally matched non‐cultured and cultured pig embryos was found at the early blastocyst stage. One possible explanation for the lower Δψ m in cultured embryos may be because of the several‐fold higher glucose concentration in NCSU‐23 medium in comparison with the oviductal fluid (Nichol et al. 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The most important difference in mitochondrial membrane potential between developmentally matched non-cultured and cultured pig embryos was found at the early blastocyst stage. One possible explanation for the lower Dw m in cultured embryos may be because of the several-fold higher glucose concentration in NCSU-23 medium in comparison with the oviductal fluid (Nichol et al 1998). This likely decreases the Krebs cycle metabolism of pyruvate, glutamine and glucose (Swain et al 2002), reduces oxidation rates of fatty acids (Tsujii et al 2001) and consequently maintains a higher lipid content in the cultured pig blastocyst ) in comparison with its in vivo counterpart.…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial Activity and Morphology in Developing Porcine Oocytes and Pre‐implantation Non‐Cultured and Cultured Embryos

Romek

Gajda

Rolka

et al. 2010

Reprod Domestic Animals

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Mitochondria are important determinants of developmental competence for oocytes and embryos owing to their central role in cellular metabolism, yet mitochondrial activity and morphometry during early porcine development have not been quantified. In this study, we examined the membrane potential Δψ(m) and the surface density Sv(in,m) of the inner mitochondrial membrane in pig oocytes and pre-implantation embryos using fluorescent probes and confocal microscopy. Mitochondria and their cristae were also examined by transmission electron microscope. Δψ(m) was consistently low from immature oocytes up to morulae and increased significantly in the early blastocyst before decreasing at the expanded blastocyst stage. This stage-dependent pattern of Δψ(m) changes differs from that reported for other mammals. We also determined that Δψ(m) is lower in cultured when compared to non-cultured porcine early blastocysts. Sv(in,m) was higher in immature oocytes than mature oocytes and remained constant up to the 4- to 8-cell embryo stage. It increased significantly at morula and early blastocyst stages. No differences in Sv(in,m) were found between developmentally matched non-cultured and cultured embryos. These results indicate that the inner mitochondrial membrane potential and surface density change significantly during pre-implantation porcine development in relation to metabolic alterations of the embryo. It is possible that modification of Δψ(m) by manipulating culture conditions may improve the performance of embryos that develop in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mitochondrial Activity and Morphology in Developing Porcine Oocytes and Pre‐implantation Non‐Cultured and Cultured Embryos

Romek

Gajda

Rolka

et al. 2010

Reprod Domestic Animals

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“…The reason for the drop in glucose is unclear but is likely due to a combination of factors that include decreased carrier-mediated transport across oviductal epithelium (Edwards and Leese, 1993), increased utilization of glucose by oviductal tissues (Nichol et al, 1992), and increased oviductal fluid secretion (Leese et al, 2001) resulting in the dilution of luminal glucose. In any case, the drop in glucose in oviductal fluid does not appear to be related to the presence of gametes or embryos (Nichol et al, 1998). Probably not coincidentally, culture media that either lack or have minimal glucose appear to be beneficial for sperm capacitation Albarracin et al, 2004) and early embryo development (Quinn et al, 1995).…”

Section: Introductionmentioning

confidence: 91%

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Tollner¹,

VandeVoort²,

Yudin³

et al. 2008

Molecular Reproduction Devel

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Capacitation of macaque sperm in vitro has been achieved efficiently only with the addition of both cyclic nucleotides and methylxanthines. The use of these exogenous sperm activators clouds an understanding of the normal mechanisms underlying capacitation and may slow early embryo development following in vitro fertilization (IVF). We demonstrate that culture medium which simulates periovulatory oviductal fluid with respect to bicarbonate (HCO(3)(-)) and glucose concentration induces capacitation in a high percentage of macaque sperm as determined by the ability of sperm to undergo both the release of coating protein DEFB126 and the zona pellucida-induced acrosome reaction (AR). Few sperm were able to undergo the AR following 6 hr incubation in medium containing either 35 mM HCO(3)(-) (approximately 7.2 pH) or 90 mM HCO(3)(-) (approximately pH 7.8) with 5 mM glucose. When glucose concentration was lowered to 0.5 mM to match levels reported for women at midcycle, the AR rate increased significantly in sperm incubated in both levels of HCO(3)(-), indicating that glucose interferes with sperm responsiveness to increasing HCO(3)(-) concentration observed in the primate oviduct during ovulation. Even greater synchronization of capacitation could be achieved with nonphysiologic extremes of alkalinity or energy substrate deprivation. In the latter case, sperm achieved high rates of IVF. A shift in pH from 7.2 to 7.8 in a HEPES-buffered medium was sufficient to remove DEFB126 from the surface of most sperm after only 3 hr. The loss of DEFB126 from sperm under periovulaory fluid conditions has implications for the timing of release of sperm from the oviductal reservoir.

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“…La libération des molécules intracytoplasmiques dans la lumière tubaire, consécutive à la nécrose, est la cause d'une surestimation de leur concentration. Le temps entre la mort et le prélèvement doit être le plus court possible, de l'ordre de la minute (Li et al 2007 (Hugentobler et al 2007b), alors que chez la femme, elle se situe aux alentours de 25 mM (Ménezo & Guérin 1997 et chez la lapine (Ménezo & Guérin 1997), la concentration du glucose varie entre 1 et 3 mM, alors que chez la truie, elle est cinq fois plus élevée (Nichols et al 1998).…”

Section: Récupération D'oviductes à L'abattoirunclassified

“…Ainsi, dans toutes les espèces, le volume des sécrétions tubaires ainsi que la concentration de leurs différents constituants sont augmentés lors de la phase oestrogé-nique du cycle (oestrus) par rapport à la phase lutéale. Chez la truie, la concentration de glucose diminue de dix fois après l'ovulation (Nichols et al 1998) et de six chez la femme, entre la phase folliculaire et le milieu de la phase lutéale (Gardner et al 1996). Des concentrations plus importantes en acides aminés pendant l'oestrus dans le fluide tubaire par rapport au plasma traduisent l'existence d'un transport actif hormonodé-pendant dans cette espèce, comme chez la brebis (Nancarrow et al 1992) et la jument (Engle et al 1984).…”

Section: Influence Hormonaleunclassified

Microenvironnement tubaire: rôle dans la maturation des ovocytes canins in vivo et in vitro

Fontaine¹,

Reynaud²,

Thoumire³

et al. 2009

bavf

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Chez la plupart des mammifères, les ovocytes sont bloqués en métaphase II au moment de l'ovulation et cette inhibition de la méiose est ensuite levée par la fécondation. Chez les chiennes et les autres femelles de canidés, les ovocytes sont libérés au stade de prophase I, et il faut encore attendre 48 à 72 heures pour qu'ils atteignent le stade de métaphase II et deviennent fécondables. Cette particularité constitue aujourd'hui un frein au développement des biotechnologies de la reproduction chez les canidés. En effet, dans les essais de maturation in vitro d'ovocytes canins, seuls 10 à 30 % des ovocytes atteignent le stade de métaphase au bout de 72 heures de culture. Chez la chienne, la maturation nucléaire se produisant dans l'oviducte, des substituts de l'oviducte (milieux de culture comme le Synthetic Oviductal Fluid, explants d'oviductes, cultures sur tapis de cellules tubaires) ont été utilisés pour les cultures d'ovocytes in vitro, afin d'en améliorer le rendement, mais sans grand succès jusqu'à maintenant. Cet échec peut être dû au manque de données sur la composition du liquide tubaire de la chienne. L'étude de ce microenvironnement prend donc tout son intérêt, celui-ci étant probablement assez différent de celui des autres femelles, ne serait-ce que par l'existence du processus de lutéinisation préovulatoire dans cette espèce. À terme, la conception d'un milieu de maturation sur la base de la composition du liquide tubaire pourrait être une voie intéressante pour augmenter les taux de maturation in vitro. Mots

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Concentrations of energy substrates in oviduct fluid in unilaterally ovariectomised pigs

Cited by 12 publications

References 16 publications

Mitochondrial Activity and Morphology in Developing Porcine Oocytes and Pre‐implantation Non‐Cultured and Cultured Embryos

Mitochondrial Activity and Morphology in Developing Porcine Oocytes and Pre‐implantation Non‐Cultured and Cultured Embryos

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Microenvironnement tubaire: rôle dans la maturation des ovocytes canins in vivo et in vitro

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