Concentrations of fetuin-A, osteoprotegerin and α-Klotho in patients with alcoholic liver cirrhosis

Prystupa, Andrzej; Dabrowska, Anna; Sak, Jarosław; Tarach, Jerzy S.; Toruń-Jurkowska, Anna; Lachowska-Kotowska, Patrycja; Dzida, Grzegorz

doi:10.3892/etm.2016.3754

Cited by 21 publications

(16 citation statements)

References 39 publications

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“…However, in order to confirm the usefulness of OSGIN1 determinations in ALD, further research conducted on larger groups of patients diagnosed with alcoholic cirrhosis are necessary. In the scientific literature, a similar postulate is reported in relation to the alpha-klotho protein [28][29][30]. One of the limitations of the current study is the small number of research subjects.…”

Section: Discussionsupporting

confidence: 70%

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Sak¹,

Prystupa²,

Bis-Wencel³

et al. 2021

Ann Agric Environ Med.

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Introduction andObjective. Human oxidative stress-induced growth inhibitor 1 (OSGIN1) is a protein identified in 2001 which belongs to the OKL38 protein family. The aim of the study was to investigate the levels of this protein depending on the severity of alcohol-induced liver cirrhosis. Materials and method. The study group consisted of 60 patients: 30 patients with cirrhosis in the P-Ch A and B stage and 30 in the P-Ch C stage. The control group consisted of 18 healthy individuals without liver diseases, who did not abuse alcohol. Oxidative stress induced growth inhibitor 1 (OSGIN1), fibroblast growth factor 1 (FGF1) and fibroblast growth factor 21 (FGF21) were determined in blood serum using enzyme-linked immunosorbent assay (ELISA) kits. All absorbance readings were conducted using an Epoch Microplate Spectrophotometer (BioTek Instrumentals, Inc., Winooski, VT, USA). OSGIN1, FGF1 and FGF21 concentrations were determined using Sandwich enzyme immunoassay kits (by Cloud Clone Corp., Katy, TX, USA). Statistica 13.3 (TIBCO Software, Inc.) was used for data analysis. Results. The concentration of OSGIN1 was 0.028 ± 0.017 in the control group which increased with the advancement of liver cirrhosis (stage of Pugh-Child): 0.075 ± 0.098 in the P-Ch A + B group and 0.121 ± 0.134 in the P-Ch C stage. Multiple comparison tests confirmed statistically significant differences in OSGIN1 concentration between the control group and P-Ch C (p <0.02). Significant correlations were noted between OSGIN1 and FGF1 (r = 0.39; p = 0.004) and between OSGIN1 and FGF21 (r = 0.53; p <0.0001). Conclusions. The study revealed that the level of OSGIN1 increased significantly in the P-Ch C stage of liver cirrhosis. It is possible that OSGIN1 may be used for the non-invasive diagnosis of ALD, but its possible diagnostic value is still very uncertain.

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Section: Discussionsupporting

confidence: 70%

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Sak¹,

Prystupa²,

Bis-Wencel³

et al. 2021

Ann Agric Environ Med.

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“…TNFRSF11b mRNA was shown in liver tissue before but it did not correlate with the higher levels of OPG found in the serum of patients with primary biliary cirrhosis compared to controls, suggesting the excess OPG is coming from a different source [11]. We therefore first assessed whether the previously reported elevated levels of OPG in liver fibrosis were a result of elevated production within the fibrotic liver or may be coming from other parts of the body [8,9]. We indeed found higher levels of OPG in human liver tissue lysates of cirrhotic livers as compared to control livers, which may point at liver production.…”

Section: Discussionmentioning

confidence: 97%

“…Several studies have pointed towards osteoprotegerin (OPG) as a novel clinical biomarker associated with liver diseases [7]. Higher serum levels were measured in patients with alcoholic liver cirrhosis, primary biliary cirrhosis, and nonalcoholic steatohepatitis, and OPG was found to correlate with disease severity in some of these studies [8][9][10][11][12][13][14][15]. Furthermore, OPG was included as an additional parameter in a panel of markers in the Coopscore© to increase the diagnostic accuracy of this test [16].…”

Section: Introductionmentioning

confidence: 99%

Osteoprotegerin Is more than a Possible Serum Marker in Liver Fibrosis: A Study into Its Function in Human and Murine Liver

et al. 2020

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Osteoprotegerin (OPG) serum levels are associated with liver fibrogenesis and have been proposed as a biomarker for diagnosis. However, the source and role of OPG in liver fibrosis are unknown, as is the question of whether OPG expression responds to treatment. Therefore, we aimed to elucidate the fibrotic regulation of OPG production and its possible function in human and mouse livers. OPG levels were significantly higher in lysates of human and mouse fibrotic livers compared to healthy livers. Hepatic OPG expression localized in cirrhotic collagenous bands in and around myofibroblasts. Single cell sequencing of murine liver cells showed hepatic stellate cells (HSC) to be the main producers of OPG in healthy livers. Using mouse precision-cut liver slices, we found OPG production induced by transforming growth factor β1 (TGFβ1) stimulation. Moreover, OPG itself stimulated expression of genes associated with fibrogenesis in liver slices through TGFβ1, suggesting profibrotic activity of OPG. Resolution of fibrosis in mice was associated with decreased production of OPG compared to ongoing fibrosis. OPG may stimulate fibrogenesis through TGFβ1 and is associated with the degree of fibrogenesis. It should therefore be investigated further as a possible drug target for liver fibrosis or biomarker for treatment success of novel antifibrotics.

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“…However, they do find significantly increase levels of OPG in cirrohotic patients as compare to control. 16 We also compare levels of OPG with sign and symptoms of chronic liver disease, and found a significant positive association of OPG with splenomegaly, hepatomegaly, skin pigmentation, malena, and prolong bleeding. The reason could be as these sign, symptoms appear at later stages of the disease, and OPG levels were high as the severity of the disease rises according to Child-Pugh’s classification.…”

Section: Discussionmentioning

confidence: 89%

Association of serum osteoprotegerin with severity of chronic liver disease in female patients: A potential biomarker

Tariq

Hussain

et al. 2020

Pak J Med Sci

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Objective: To determine the association of serum osteoprotegerin (OPG) with the severity of chronic liver disease in female patients. Methods: This case-control study was conducted in Madina Teaching Hospital from 2019-2020.An institutional review board of University Medical and Dental College, The University of Faisalabad gave the approval to conduct the study. Only female patients of age group 40 to 60 years having CLD were included in this study. Total 80 participants were enrolled after fulfilling the inclusion and exclusion criteria. Serum OPG levels were measured by enzyme linked immunosorbant assay (ELISA) supplied by ELAB Sciences, USA. The severity of disease was assessed by Child-Pugh classification. Results: OPG levels were significantly different between the three Child-Pugh classes. OPG levels were significantly high in class C indicating increased level of this cytokine in CLD as compared to class A (p = <0.05). There was a positive association of OPG with splenomegaly (OR = 2.10, p = <0.001), hepatomegaly (OR = 4.41, (p = < 0.05), skin pigmentation (OR = 2.06, p = <0.05), malena (OR = 1.87, p = <0.05) and prolonged bleeding (OR = 1.86, p = <0.05). Conclusion: The levels of serum Osteoprotegerin is increased in severe form of chronic liver disease (Class C) of Child-Pughs classification as compared to mild (Class A) and moderate (Class B) forms of Child-Pughs classification. doi: https://doi.org/10.12669/pjms.36.6.2678 How to cite this:Tariq S, Tariq S, Hussain S, Baig M. Association of serum osteoprotegerin with severity of chronic liver disease in female patients: A potential biomarker. Pak J Med Sci. 2020;36(6):---------. doi: https://doi.org/10.12669/pjms.36.6.2678 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Concentrations of fetuin-A, osteoprotegerin and α-Klotho in patients with alcoholic liver cirrhosis

Cited by 21 publications

References 39 publications

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Osteoprotegerin Is more than a Possible Serum Marker in Liver Fibrosis: A Study into Its Function in Human and Murine Liver

Association of serum osteoprotegerin with severity of chronic liver disease in female patients: A potential biomarker

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