Concentrations of lactoferrin and iron in human milk at different stages of lactation.

Hirai, Yukihiko; N, Kawakata; Satoh, K.; Ikeda, Yuki; Hisayasu, Sanae; Orimo, Hideo; Yoshino, Yoshio

doi:10.3177/jnsv.36.531

Cited by 79 publications

(41 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recovery studies showed close agreement between the theoretical and measured protein concentrations in dilution-overloadind assays (linear recovery on large ranges of concentrations with mean percentage very close to 100%). Results of the measurements of nine proteins performed together in a great number of human mature milk samples are, for the most part, in good agreement with those previously reported in past years (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), using different techniques and, the most often, restricted to the determination of the one or another of these proteins. Emergence of new analytical procedures in the late years, such as conventional or microparticleenhanced immunonephelometry, today allows major proteins of human milk to be analyzed with sensitivity, easiness, and singleness of methodologies.…”

Section: Discussionsupporting

confidence: 77%

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Montagne¹,

Trégoat²,

Cuillière³

et al. 2000

Clinical Biochemistry

View full text Add to dashboard Cite

Objectives: Microparticle-enhanced nephelometric immunoassays for six human milk proteins (␤-casein, -casein, ␣-lactalbumin, serum albumin, lactoferrin, and lysozyme) and conventional immunonephelometry assays for immunoglobulin A, C3, and C4 complement proteins were developed and characterized. Design and methods: Microparticle-enhanced nephelometric immunoassays are competitive assays based on the nephelometric quantification of the inhibition of microparticle-protein conjugates immunoagglutination by the proteins to be assayed. Results: High precision (CVs ranged from 1% to 14% in within-and between-assays) and recovery (linear recovery in dilution-overloading assay) ensure a reliable determination of the main human milk proteins by single-step homogeneous nephelometric immunoassays, accurate over wide ranges of concentration. These immunoassays were easily applied to a large number of mature human milk samples (between 373 and 503 according to the proteins tested). Conclusions:The immunoassays developed could be applied to the fast determination of human milk protein profile usable for nursery milk bank and fortification.

show abstract

Section: Discussionsupporting

confidence: 77%

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Montagne¹,

Trégoat²,

Cuillière³

et al. 2000

Clinical Biochemistry

View full text Add to dashboard Cite

show abstract

“…Sucrase and lactase show a sugar-dependent rapid increase in the first days of life (6). Interestingly, a rapid increase is observed in disaccharidase activities in jejunal fluid during the first, second, and third weeks of life (30) in parallel with the decrease in LF in human milk (15). We speculate that the higher concentrations of LF in colostrum contributes to the early proliferation of intestinal cells, which then differentiate as a result of its decreased concentration.…”

mentioning

confidence: 92%

“…LF, an iron-binding 80-kDa glycoprotein (12), is found in amniotic fluid and mammalian milk in iron-saturated and iron-unsaturated forms (13,14). Its concentration in human milk is related to infant age: it peaks in colostrum and rapidly decreases in mature milk (15). It has been reported that LF resists proteolysis through the infant's digestive tract (16) and binds to a specific receptor located on the enterocyte brush-border (17).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Lactoferrin Induces Concentration-Dependent Functional Modulation of Intestinal Proliferation and Differentiation

et al. 2007

View full text Add to dashboard Cite

Human milk stimulates intestinal development through the effects of various moieties. Lactoferrin (LF) is a glycoprotein of human milk whose concentration is highest in colostrum decreasing in mature milk. LF promotes enterocyte growth in intestinal cell lines. We tested the hypothesis that LF induces a distinct effect on enterocyte proliferation and differentiation, depending on its concentration. We examined the dose-related effects by humannative LF (N-LF) in Caco-2 (human colon adenocarcinoma) cells. At high concentrations, N-LF stimulated cell proliferation in immature Caco-2 cells, as judged by 3 H-thymidine incorporation. In contrast, sucrase and lactase activities were increased at low but not high LF concentrations and their mRNA were also increased, indicating a transcriptional effect. Because iron binds specific LF sites, we compared the potency of N-LF and iron-saturated LF (I-LF) and found the native form more potent. Finally, we tested the effects by bovine LF (bLF) in the same system and found the latter more potent than the human isoform in inducing cell growth and lactase expression. These results suggest that LF directly induces enterocyte growth and proliferation, depending on its concentration, thereby regulating the earlyx postnatal intestinal development. bLF could be added to infant formula as a growth factor in selected intestinal diseases.

show abstract

“…56,57 It is abundant in colostrum at up to 7 g/L, and its concentration declines sevenfold as lactation progresses. [58][59][60] After pasteurization at 62.5°C for 30 minutes, only 39% of the original lactoferrin remains in the milk. 27 This major protein of human milk chelates free iron, which may assist in iron absorption by the infant, and iron chelation also inhibits bacterial growth.…”

Section: Lactoferrinmentioning

confidence: 99%

Human Milk Glycoproteins Protect Infants Against Human Pathogens

Liu

Newburg

2013

Breastfeeding Medicine

139

104

View full text Add to dashboard Cite

Breastfeeding protects the neonate against pathogen infection. Major mechanisms of protection include human milk glycoconjugates functioning as soluble receptor mimetics that inhibit pathogen binding to the mucosal cell surface, prebiotic stimulation of gut colonization by favorable microbiota, immunomodulation, and as a substrate for bacterial fermentation products in the gut. Human milk proteins are predominantly glycosylated, and some biological functions of these human milk glycoproteins (HMGPs) have been reported. HMGPs range in size from 14 kDa to 2,000 kDa and include mucins, secretory immunoglobulin A, bile salt-stimulated lipase, lactoferrin, butyrophilin, lactadherin, leptin, and adiponectin. This review summarizes known biological roles of HMGPs that may contribute to the ability of human milk to protect neonates from disease.

show abstract

Concentrations of lactoferrin and iron in human milk at different stages of lactation.

Cited by 79 publications

References 33 publications

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Measurement of nine human milk proteins by nephelometric immunoassays: application to the determination of mature milk protein profile

Lactoferrin Induces Concentration-Dependent Functional Modulation of Intestinal Proliferation and Differentiation

Human Milk Glycoproteins Protect Infants Against Human Pathogens

Contact Info

Product

Resources

About