Concentrations of Neutrophil-Derived Proteins in Meconium and Their Correlations

Lisowska-Myjak, Barbara; Żytyńska-Daniluk, Joanna; Skarżyńska, Ewa

doi:10.2217/bmm-2016-0034

Cited by 13 publications

(11 citation statements)

References 36 publications

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“…In fact, throughout and immediately after birth, infants are exposed to multiple microbial communities derived from the external environment [51]. Moreover, the composition of the new-born's microbiome is initially set not only by maternal microbiome transfer but also by birth mode, early-life feeding practices [52] and delivery mode [53].…”

Section: Discussionmentioning

confidence: 99%

Prenatal and postnatal determinants in shaping offspring's microbiome in the first year of life. Preliminary results.

Raspini¹,

Porri²,

Giuseppe³

et al. 2019

Preprint

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Background Fetal programming during in utero life defines the set point of physiological and metabolic responses that lead into adulthood; events happening in “the first 1,000 days” play a role in the development of non-communicable diseases (NCDs). The infant gut microbiome is a highly dynamic organ, which is sensitive to maternal factors and environmental insults; it modifies its composition over the host’s lifespan and is one of the elements driving this intergenerational NCDs' transmission. The A.MA.MI (Alimentazione MAmma e bambino nei primi MIlle giorni) project aims at investigating the possible correlation between pre-natal and post-natal factors and the infant gut microbiome composition, during the first year of life at different follow-up. We describe the study design of the A.MA.MI Study and present some preliminary results.Methods A.MA.MI is a longitudinal, prospective, observational study that includes a group of mother-infant pairs (n=63) attending the Neonatal Unit, Fondazione IRCCS Policlinico San Matteo, Pavia (Italy). The study was planned to provide data collected before discharge (T0) and at 1,6,12 months after birth (T1,T2,T3). Maternal and infant anthropometric measurements are assessed at each time. Other variables evaluated are pre-pregnancy/gestational weight status (T0), maternal dietary habits/physical activity (T1-T3); infant medical history, type of feeding, antibiotics/probiotics/supplements use, environment exposures (e.g cigarette smoking, pets, environmental temperature) (T1-T3). A child stool sample was planned to be collected at each time and analyzed using metagenomics 16S ribosomal RNA gene sequence-based methods. Maternal urine samples were planned to be collected at T3 to investigate pollutants exposure (Phthalates, Bisphenol A and Hydroxypyrene).Results Concerning the birth mode (cesarean section vs. vaginal delivery) significant differences were found only at genera and species levels (T0). A significantly higher relative abundance of Firmicutes was found in meconium of infants born from mothers affected by overweight/obesity, when compared to women with normal weight before pregnancy (T0). Regards type of feeding (breastfed vs formula-fed) the gut microbiota composition differed significantly only at genus and species level (T1).Conclusion These preliminary and explorative results confirmed that pre-pregnancy BMI, mode of delivery and infant factors could affect the infant microbiota composition at different levels.

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Section: Discussionmentioning

confidence: 99%

Prenatal and postnatal determinants in shaping offspring's microbiome in the first year of life. Preliminary results.

Raspini¹,

Porri²,

Giuseppe³

et al. 2019

Preprint

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“…Fecal MPO in adults is reportedly a parameter of IBD 18 and ulcerative colitis severity 19 . The correlation between calprotectin and MPO in neonates was demonstrated 20 . Fecal eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are often seen in a patient with food allergic colitis and typically present in children with an atopic family history before the age of two years 21 .…”

Section: Introductionmentioning

confidence: 95%

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

Vidová

Benesová

Klánová

et al. 2021

Sci Rep

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An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha-1-antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (> 100; typically 0.04 to 14.77 µg/mg of total protein), coefficient of determination (R2; > 0.99), the limit of detection (LOD; 0.003 to 0.04 µg/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 µg/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.

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“…Calprotectin (36.5 kDa), a heterodimeric (S100A8/A9) calcium-and zinc-binding protein which makes up 5 % of the total protein and 60% of the cytosolic protein of neutrophils. It is present in meconium at higher (even >10 fold) levels than in feces of children over 1 year of age and adults [3,16].…”

Section: Introductionmentioning

confidence: 96%

“…As the first feces of the newborn it is noninvasively collected in the first 24-48 hours after birth. It is a carrier of numerous chemical compounds and metabolites, including specific proteins with different biological properties and functions during gestation [ 3,4]. In the current laboratory diagnostics, the potential properties of meconium as a source of specific parameters for assessing the importance of intrauterine development on the health of the newborn are not sufficiently understood or practically used.…”

Section: Introductionmentioning

confidence: 99%

“…Proteomics has confirmed the complexity of meconium proteins [3,4]. Selection of certain individual proteins detected in meconium, which interact with each other in the performance of their biological functions, may offer a new diagnostic panel and provide knowledge about their role(s) in maintaining homeostasis of the intrauterine environment.…”

Section: Introductionmentioning

confidence: 99%

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Links Between Vitamin D-Binding Protein, Alpha-1 Antitrypsin and Neutrophil Proteins in Meconium

Lisowska-Myjak

Skarżyńska

Jakimiuk

2023

Cell Physiol Biochem

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Background/Aims: Alpha-1 antitrypsin (AAT), vitamin-D binding protein (VDBP) and neutrophil granule proteins are specifically related to the neutrophil function and may be considered candidate biomarkers detected and measured in meconium (the first feces of newborn infants) as signals indicating abnormal processes in the fetal stage. Individual proteins found in meconium can be a source of information pertaining to the intrauterine metabolic processes. Methods: Concentrations of AAT, VDBP, calprotectin, myeloperoxidase, lactoferrin and elastase were measured using ELISA tests in 80 meconium samples collected from 19 healthy, full-term neonates. Results: The meconium concentrations of VDBP and AAT (mean±SD, [mg/g meconium]: 3.74±6.93, 3.72±1.79, respectively) were approximately 1000 times higher than those of the protein granule proteins calprotectin, myeloperoxidase, elastase and lactoferrin (mean ± SD, [µg/g meconium]: 285.7±215.8, 1.83±1.73, 1.72±2.70, 45.58±78.89, respectively). The correlation between VDBP and AAT was negative (r= - 0.40. p=0.000) and those between VDBP and calprotectin (r=0.38, p=0.000) and VDBP and myeloperoxidase (r=0.45, p=0.000) were positive. AAT was found to correlate positively with lactoferrin (r=0.38, p=0.000). Conclusion: The correlations between the concentrations of VDBP and AAT, and with neutrophil granule proteins observed in meconium indicate their functional relationship in the intrauterine environment of the developing fetus. Meconium can be seen as an apparently underutilized source of biomarkers for evaluation of metabolic processes specific to fetal development.

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Concentrations of Neutrophil-Derived Proteins in Meconium and Their Correlations

Abstract: Calprotectin, lactoferrin, myeloperoxidase and PMN-elastase concentrations in meconium are interrelated. These proteins may serve as objective biomarkers describing and/or assessing the intrauterine environment.

Cited by 13 publications

References 36 publications

Prenatal and postnatal determinants in shaping offspring's microbiome in the first year of life. Preliminary results.

Prenatal and postnatal determinants in shaping offspring's microbiome in the first year of life. Preliminary results.

Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation

Links Between Vitamin D-Binding Protein, Alpha-1 Antitrypsin and Neutrophil Proteins in Meconium

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