Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Dymock, D.C.; Brown, M. P.; Merritt, K.A.; Trumble, Troy N.

doi:10.2460/ajvr.75.8.722

Cited by 11 publications

(15 citation statements)

References 41 publications

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“…They suggest that greater background non-specific binding in serum samples masks small changes in cytokine concentrations that are better detected using plasma, and that ‘higher’ is not necessarily better. In contrast, Dymock et al (Dymock et al 2014) concluded that stromal cell-derived factor 1 measured in serum was more sensitive than plasma or synovial fluid in distinguishing horses with osteochondral injury from uninjured horses. In the current study, the objective was to compare the values obtained from serum and plasma in cats, however future work should evaluate the relative sensitivity of serum and plasma to detect changes in cytokine concentrations in disease in a larger sample of cats.…”

Section: Resultsmentioning

confidence: 96%

“…Despite this, obtaining whole blood is more convenient than most other sampling modalities, particularly in clinical cases, and offers a potential for screening large populations. In fact, concentrations of several cytokines have been found to be altered in the serum and plasma of patients during disease, including IL-6 in humans with rheumatoid arthritis (Burska, Boissinot, and Ponchel 2014) and dogs with immune-mediated polyarthritis (Foster et al 2014), and stromal cell-derived factor-1 in horses with osteochondral injury (Dymock et al 2014). These reports pertain to serum or plasma samples, but the two sample types are not necessarily interchangeable.…”

Section: Introductionmentioning

confidence: 99%

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A comparison of serum and plasma cytokine values using a multiplexed assay in cats

Gruen

Messenger

Thomson

et al. 2016

Veterinary Immunology and Immunopathology

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Background Degenerative joint disease (DJD) is highly prevalent in cats, and pain contributes to morbidity. In humans, alterations of cytokine concentrations have been associated with joint deterioration and pain. Similar changes have not been investigated in cats. Cytokine concentrations can be measured using multiplex technology with small samples of serum or plasma, however, serum and plasma are not interchangeable for most bioassays. Correlations for cytokine concentrations between serum and plasma have not been evaluated in cats. Objective To evaluate the levels of detection and agreement between serum and plasma samples in cats. Animals Paired serum and plasma samples obtained from 38 cats. Methods Blood was collected into anti-coagulant free and EDTA Vacutainer® tubes, serum or plasma extracted, and samples frozen at −80°C until testing. Duplicate samples were tested using a 19-plex feline cytokine/chemokine magnetic bead panel. Results Agreement between serum and plasma for many analytes was high, however correlation coefficients ranged from −0.01 to 0.97. Results from >50% of samples were below the lower limit of quantification for both serum and plasma for nine analytes, and for an additional three analytes for plasma only. Conclusions and clinical importance While serum and plasma agreement was generally good, detection was improved using serum samples.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

A comparison of serum and plasma cytokine values using a multiplexed assay in cats

Gruen

Messenger

Thomson

et al. 2016

Veterinary Immunology and Immunopathology

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“…Data from multiple species demonstrate production of inflammatory mediators post-injury, including cytokines and chemokines observed in human patients [26-30]. Recent studies address how inflammatory gene expression varies with time after injury in common rodent models.…”

Section: Methodsmentioning

confidence: 99%

Inflammation in joint injury and post-traumatic osteoarthritis

Lieberthal

Sambamurthy

Scanzello

2015

Osteoarthritis and Cartilage

380

350

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Inflammation is a variable feature of osteoarthritis (OA), associated with joint symptoms and progression of disease. Signs of inflammation can be observed in joint fluids and tissues from patients with joint injuries at risk for development of post-traumatic osteoarthritis (PTOA). Furthermore, inflammatory mechanisms are hypothesized to contribute to the risk of OA development and progression after injury. Animal models of PTOA have been instrumental in understanding factors and mechanisms involved in chronic progressive cartilage degradation observed after a predisposing injury. Specific aspects of inflammation observed in humans, including cytokine and chemokine production, synovial reaction, cellular infiltration and inflammatory pathway activation, are also observed in models of PTOA. Many of these models are now being utilized to understand the impact of post-injury inflammatory response on PTOA development and progression, including risk of progressive cartilage degeneration and development of chronic symptoms post-injury. As evidenced from these models, a vigorous inflammatory response occurs very early after joint injury but is then sustained at a lower level at the later phases. This early inflammatory response contributes to the development of PTOA features including cartilage erosion and is potentially modifiable, but specific mediators may also play a role in tissue repair. Although the optimal approach and timing of anti-inflammatory interventions after joint injury are yet to be determined, this body of work should provide hope for the future of disease modification tin PTOA.

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“…37 In horses with osteochondral injuries, serum levels were decreased whereas synovial fluid concentrations increased in comparison with uninjured animals. 38 Shen and coworkers recently studied the effects of human meniscus-derived stem/progenitor cells (hMeSPCs) in a rat menscectomy model. These cells were injected intra-articularly 1 week after meniscectomy, and homed to the injured meniscus.…”

Section: Ccl19/21mentioning

confidence: 99%

Chemokines and inflammation in osteoarthritis: Insights from patients and animal models

Scanzello

2017

Journal Orthopaedic Research

174

140

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Evidence has been building that the pathologic drive for development of osteoarthritis (OA) involves more than simple mechanical “wear and tear.” Inflammatory mechanisms play an important role in the tissue response to joint injury, and are involved in development of post-traumatic OA. Inflammation also appears integral to the progression of OA, whether post-traumatic or spontaneous, contributing to the evolution of joint tissue degradation and remodeling as well as joint pain. Both patient-based studies and in vivo models of disease have shed light on a number of inflammatory pathways and mediators that impact various aspects of this disease, both structurally and symptomatically. Recent work in this field has implicated inflammatory chemokines in osteoarthritis pathogenesis. Expression of multiple chemokines and their receptors is modulated during disease in both patients and animal models. Although best known for their effects on leukocyte migration and trafficking within the immune system, chemokines can have a wide variety of effects on both motile and non-motile cell types, impacting proliferation, differentiation, and activation of cellular responses. Their role in OA models has also demonstrated diverse effects on disease that exemplify their wide-ranging effects. Understanding how these important mediators of inflammation impact joint disease, and whether they can be targeted therapeutically, is actively being investigated by many groups in this field. This narrative review focuses on evidence published within the last 5 years highlighting chemokine-mediated pathways with mechanistic involvement in osteoarthritis and joint tissue repair.

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Concentrations of stromal cell-derived factor-1 in serum, plasma, and synovial fluid of horses with osteochondral injury

Cited by 11 publications

References 41 publications

A comparison of serum and plasma cytokine values using a multiplexed assay in cats

A comparison of serum and plasma cytokine values using a multiplexed assay in cats

Inflammation in joint injury and post-traumatic osteoarthritis

Chemokines and inflammation in osteoarthritis: Insights from patients and animal models

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