Concentrations of uterine luminal prostaglandins in mares with acute and persistent endometritis

Watson, E.D.; Stokes, C.R.; David, Juan; Bourne, F.J.; Ricketts, S. W.

doi:10.1111/j.2042-3306.1987.tb02574.x

Cited by 52 publications

(25 citation statements)

References 22 publications

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“…The assay used for PGF has been described previously (Watson et al, 1987). Assays for PGE-2 and 6-keto-PGF-la were performed using an iodinated tracer and reagents from New England Nuclear (N. Billerica, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares

Watson

Sertich²

1989

Reproduction

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Summary. Embryos, endometrial biopsies, and uterine lavage fluid were collected from pregnant and non-pregnant mares 14 days after ovulation. Embryos were cultured for 20\m=.\5h with and without endometrial tissue from pregnant mares, and endometrial tissue was cultured alone. Endometrial content of PGF tended to be higher (P = 0\m=.\06) in non-pregnant than in pregnant mares, but the amount of PGF released from tissue during culture was similar for pregnant and non-pregnant mares. Lavage fluid from non-pregnant mares also tended (P= 0\m=.\08) to contain higher concentrations of PGF. Co\ x=req-\ incubation of embryos with endometrium from pregnant mares significantly (P = 0\m=.\01) lowered concentrations of PGF in medium. Tissue concentrations and release of PGE-2 and 6-keto-PGF-1\g=a\were similar in endometrial samples from pregnant and non\x=req-\ pregnant mares and prostaglandin production was unaffected by the presence of an embryo during incubation. Horse embryos released all three prostaglandins during a 20\m=.\5-hincubation.

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Section: Methodsmentioning

confidence: 99%

Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares

Watson

Sertich²

1989

Reproduction

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“…Both IgG and C3 declined during this time, which may be explained by active opsonization of bacteria. A rapid migration of PMNs into the uterine lumen in response to a uterine infection has been reported by Watson et al [16] and by Pycock and Allen [23]. Optimal function of PMNs requires opsonization of the infectious agent before it can be phagocytized and killed.…”

Section: Discussionmentioning

confidence: 89%

“…Mares susceptible to CUI have been proposed to have elevated concentrations of immunoglobulins [9,15] and complement [16] in uterine fluids when compared to resistant mares. However, previous studies of immunloglobulin and complement concentrations in the uterus have been based on the analysis of uterine washes retrieved with an unknown dilution of fluid.…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin (IgG and IgA) and complement (C3) concentrations in uterine secretion following an intrauterine challenge of Streptococcus Zooepidemicus in mares susceptible to versus resistant to chronic uterine infection1

Troedsson

Liu

Thurmond

1993

Biology of Reproduction

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The validity of measuring concentrations of immunoglobulins in undiluted uterine secretions was established. The concentrations of IgG, IgA, and cleavage factor C3 of the complement system in uterine secretions were compared in mares with different resistance to chronic uterine infection (CUI). The uteri of mares susceptible (n = 6) and resistant to CUI (n = 5) were inoculated with 5 x 10(6) Streptococcus zooepidemicus when the mares were in estrus. Uterine secretions were sampled, and sampling was immediately followed by a uterine lavage at 5 and 24 h after bacterial inoculation. During a subsequent estrus, bacterial inoculation of the uterus was repeated, and samples were taken from the mares 12 and 36 h later. The fraction of uterine secretion in each uterine washing was determined by dividing the protein concentration of the undiluted uterine secretion by the protein concentration of the corresponding uterine washing. There was a significant correlation between measured concentrations of immunoglobulins in uterine secretions and calculated concentrations in the uterine washings (p < 0.05). Concentrations of IgG and C3 in uterine secretions declined similarly in both susceptible and resistant mares during the first 24 h after bacterial inoculation (p < 0.04). In contrast to the susceptible group, which showed a continuous decline at 36 h, resistant mares demonstrated an increased concentration of both IgG and C3 at this time. Concentrations of IgA did not differ between susceptible and resistant mares. It was concluded that the described method of sampling uterine secretions was useful for analyzing IgG and IgA.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…PGF2α and its metabolite PGFM are not only secreted during physiological luteolysis (Ginther et al, 2010) but they are also secreted after transrectal uterine manipulation (Velez et al, 1991), after performing AI/ET procedures (Scenna et al, 2005, Koblischke et al, 2008 or in cases of endometrial infections in cows (Del Vecchio et al, 1992, Archbald et al, 1998, Mateus et al, 2003 and mares (Watson et al, 1987). To evaluate whether the CL lifespan is affected by the biopsy procedure in cattle, Mann and Lamming (1994) did a repeated collection of endometrial tissues in cyclic cows (2 days interval) in the latter stages of the luteal phase (Days 13 to 17 of the ).…”

Section: Effects Of Endometrial Sampling On Uterine Healthmentioning

confidence: 99%

“…In mares, trauma caused by handling the uterine tract after performing ET or EB during metoestrus or dioestrus, induced the release of luteolytic pulses of PGF2α from the endometrium, subsequently shortening the oestrous cycle (Baker et al, 1981, Watson et al, 1987, Gilbert, 1989, Koblischke et al, 2008.…”

Section: Introductionmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Concentrations of uterine luminal prostaglandins in mares with acute and persistent endometritis

Cited by 52 publications

References 22 publications

Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares

Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares

Immunoglobulin (IgG and IgA) and complement (C3) concentrations in uterine secretion following an intrauterine challenge of Streptococcus Zooepidemicus in mares susceptible to versus resistant to chronic uterine infection1

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

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