Purpose: This study attempted to identify the extent of career anchors, professional self-concept, and work values among nursing students, as well as to provide a basis for developing educational programs to help nursing students make appropriate career decisions based on the results of the analysis of the relationship between professional self-concept and work values according to the type of career anchors. Methods: This descriptive survey study was conducted to determine the effect of career anchor type on nursing students' self-concept of the nursing profession and work values. The data collection period extended from December 1 to December 31, 2023, and the collected data were statistically analyzed using SPSS/WIN 25.0 program. Results: The results showed that technical/functional, service/commitment, and entrepreneur anchors had significant effects on nursing professional self-concept, with an explanatory power of 28.7%. The results showed that technical/functional, autonomy/independence, and service/ commitment anchors had significant effects on work values, with an explanatory power of 33.9%. Conclusion: The results of this study suggest that career anchor types should be identified and customized educational programs should be developed for nursing students to develop positive professional self-concept and work values.