Conceptus, progesterone, and breed effects on uterine protein secretion in swine.

Vallet, J. L.; Christenson, R. K.; Trout, William E.; Klemcke, Harold G.

doi:10.2527/1998.76102657x

Cited by 62 publications

(49 citation statements)

References 37 publications

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“…However, our present data estimating P4 in the caval vein and those reported from peripheral blood collection [13,14] do not confirm such a P4 increase. It has also been suggested that the P4 level on Days 2 and 3 of pregnancy influences conceptus development [29]. Our data did not find a relation between the P4 concentration on Day 3 and pregnancy outcome.…”

Section: Gnrh Agonist Effects In Pregnant Giltscontrasting

confidence: 51%

Endocrine Effects of GnRH Agonist Application to Early Pregnant Gilts

Brüssow

Schneider

Wollenhaupt

et al. 2011

Journal of Reproduction and Development

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Abstract. The hypothesis of the present study was that a GnRH agonist application at early pregnancy would alter the pattern of the key reproductive hormones LH and FSH, and subsequently that of estradiol (E2) and especially progesterone (P4), and improve the conditions for embryo survival in early pregnant gilts. Therefore, the endocrine effects of a GnRH agonist (GnRHa) application to gilts (n=11 GnRHa treated, n=9 saline Controls) were studied in blood samples from the Vena cava caudalis. GnRHa injected on Day 12 after insemination induced elevated (P<0.01) LH and FSH levels for at least 180 min. However, subsequent LH concentrations were not altered up to Day 21 of pregnancy. LH pulse number, estimated in 6-h period samples on Days 13, 15 and 17, was not influenced by treatment and pregnancy. LH pulse amplitude was decreased (P<0.05) on Days 13 to 17 in pregnant gilts of both groups, but not in nonpregnant animals. In pregnant GnRHa-treated gilts, the basal LH level was elevated compared with the Controls (P<0.01). Additionally, differences (P<0.05) in basal LH were present between the pregnant and nonpregnant animals. The P4 and E2 secretion pattern was not affected by GnRHa. P4 concentrations increased (P<0.01) from Day 10 to Day 14 regardless of the treatment. P4 revealed a pulse-like pattern, but without a definite relation to the LH pulse characteristics. Also, pregnancy rate (73 vs. 67%) and the number of fetuses (12.8 ± 2.3 vs. 11.6 ± 2.3) were unaffected in the treated and Control gilts, respectively. The present study did not confirm the initial hypothesis that a GnRHamediated LH effect could alter ovarian steroid secretion and favorably support early embryo development and pregnancy outcome. Key words: GnRH-agonist, LH, Pig, Pregnancy, Progesterone (J. Reprod. Dev. 57: [242][243][244][245][246][247][248] 2011) rogesterone from the developing corpus luteum (CL) is the most important driver of endometrial secretion and receptivity for early conceptus development and implantation in pigs [1,2]. About 30% of porcine conceptuses are lost during the peri-implantation period [3], and this period is susceptible to a lack of P4. It has been shown that a concentration of 4 ng/ml P4 in peripheral blood appears to be minimum required for maintenance of pregnancy [4]. Attempts have been undertaken to improve pregnancy rate or reduce pregnancy losses in pigs by steroid or gonadotropin application [5][6][7]. Since steroid treatment may improve litter size [8,9], the question is whether luteal P4 secretion can be enhanced by GnRH-induced LH stimulation. This is because LH is considered to be essential in pigs for support of luteal P4 production and pregnancy outcome as demonstrated by passive immunization with anti-LH serum or blockage of LH secretion and regulation of ovarian function by a GnRH antagonist [10,11].The hypothesis of the present study was that a single GnRH agonist application, given on Day 12 after insemination, would alter the pattern of the key reproductive hormones LH and FSH, and subsequentl...

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Section: Gnrh Agonist Effects In Pregnant Giltscontrasting

confidence: 51%

Endocrine Effects of GnRH Agonist Application to Early Pregnant Gilts

Brüssow

Schneider

Wollenhaupt

et al. 2011

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

“…As progesterone is involved in secretory functions in the uterus (Adams et al 1981, Roberts et al 1987, Vallet et al 1998, differences in PR immunopresence could therefore be expected. Our results support this concept by showing PR differences between surface and glandular compartments.…”

Section: Discussionmentioning

confidence: 99%

“…A stimulatory effect by progesterone on uterine protein secretion has been shown in pigs in several studies (Adams et al 1981, Roberts et al 1987, Vallet et al 1998. During pregnancy progesterone has an important role in preparations for uterine reception of the early embryos and for the maintenance of pregnancy.…”

Section: Introductionmentioning

confidence: 94%

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Sukjumlong¹,

Dalin²,

Sahlin³

et al. 2005

Reproduction

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Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5 -6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

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“…Meishan pigs are prolific breeders, averaging three to five more pigs per litter than the European breeds, even though their ovulation rates are similar (Ford and Youngs 1993;Haley et al 1995;Vallet et al 1998). Gene expression in the endometrium of different breeds may relate to reproduction traits.…”

Section: Discussionmentioning

confidence: 99%

Expression analysis of the porcine Homeobox A11 gene and its association with litter size in Large White pigs

Lin¹,

Jia²,

Chen³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The homeobox A11 (HOXA11) gene, a well-known transcriptional regulator, plays a critical role in normal development of the uterus. In this study, the porcine HOXA11 gene, including its promoter region, was cloned and sequenced (GenBank Accession No. KF724933). Differential expression levels of HOXA11 in the endometrium at one stage of the estrous cycle and three stages of gestation for Meishan and Large White pigs were investigated. Association between the single nucleotide polymorphism (SNP) and litter size was conducted. Results suggested that expression of the HOXA11 gene was the highest on day 15 and decreased on day 26 of gestation, reaching a minimum level of expression on day 50 of gestation in Large White pigs. Expression of the HOXA11 gene tended to be consistent between day 15 of the estrous cycle and day 26 of gestation, and decreased on day 50 of gestation in Meishan pigs. Series deletion analysis and reporter expression assays identified a promoter region of the HOXA11 gene which possessed a five times higher promoter activity than the pGL3-Basic vector. Using comparative sequencing, a candidate SNP (KF724933:g.2325C>A) was identified. In a Large White pig population, this polymorphism was associated with the number of piglets. In a combined analysis containing all parity groups, sows with the AC genotype had greater total number of born (1.80; P < 0.05) and number of born alive (1.82; P < 0.05) piglets compared with the CC genotype sows. These results indicate HOXA11 may play a role in the regulation of embryo implantation in pigs.

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Conceptus, progesterone, and breed effects on uterine protein secretion in swine.

Cited by 62 publications

References 37 publications

Endocrine Effects of GnRH Agonist Application to Early Pregnant Gilts

Endocrine Effects of GnRH Agonist Application to Early Pregnant Gilts

Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Expression analysis of the porcine Homeobox A11 gene and its association with litter size in Large White pigs

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