Concerning the determination of total intracellular concentrations by the cryoscopic method

Glassy State and Cryopreservation of Mint Shoot Tips

“…Equivalent solute concentrations (isoosmotic) can be obtained from the freezing equilibrium temperatures obtained by DSC . The observed decrease in this temperature can be related by Raoult's law to the molal concentration of the solution.…”

“…2 Equivalent solute concentrations (isoosmotic) can be obtained from the freezing equilibrium temperatures obtained by DSC. 55 The observed decrease in this temperature can be related by Raoult's law to the molal concentration of the solution. In the case of tissues, it would rather be an average of the different solution pools in the different cell types and subcellular compartments of the specimens studied.…”

Water Relations of the Ovarian Cycle

“…Another equally remarkable feature of osmolality is its constant level throughout body-fluid compartments. This has been convincingly demonstrated in several different ways for mammalian serum and intracellular fluid (Buckley et al, 1958;Appelboom et al, 1958;Maffly and Leaf, 1959), and cryoscopically for human serum and other body fluids in a variety of pathological conditions (Hendry, 1962). There is, therefore, every reason for believing that in both health and disease, constant osmolality, or constant water activity, prevails throughout the intracellular, extracellular and loculated spaces of the body.…”