Concise nanotherapeutic modality for cancer involving graphene oxide dots in conjunction with ascorbic acid

Shih, Chun-Yan; Wang, Pei-Ting; Chung, Weon Kuu; Wang, Wen-Hsiu; Chiang, I-Ting; Su, Wu‐Chou; Huang, Wei‐Ping; Teng, Hsisheng

doi:10.1039/d3nr00431g

Cited by 1 publication

(1 citation statement)

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“…The addition of a different shell can achieve a reduction in oxidation [ 25 ]. Free radical production is an important factor that contributes to NCs’ toxicity [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Divergent Responses of Hydrophilic CdSe and CdSe@CdS Core–Shell Nanocrystals in Apoptosis and In Vitro Cancer Cell Imaging: A Comparative Analysis

Das,

Bhatt,

Parambil

et al. 2023

JFB

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With their distinctive core–shell design, core–shell nanocrystals have drawn interest in catalysis, medicinal research, and nanotechnology. These nanocrystals have a variety of characteristics and possible uses. The application of core–shell nanocrystals offers significant potential in increasing diagnostic and therapeutic approaches for cancer research in apoptosis and in vitro cancer cell imaging. In the present study, we investigated the fluorescence behavior of hydrophilic CdSe (core-only) and CdSe@CdS (core–shell) nanocrystals (NCs) and their potential in cancer cell imaging. The addition of a CdS coating to CdSe NCs increased the fluorescence intensity tenfold. The successful fabrication of core–shell CdSe@CdS nanocrystals was proven by a larger particle size (evaluated via DLS and TEM) and their XRD pattern and surface morphology compared to CdSe (core-only) NCs. When these NCs were used for bioimaging in MCF-7 and HEK-293 cell lines, they demonstrated excellent cellular uptake due to higher fluorescence intensity within cancerous cells than normal cells. Comparative cytotoxicity studies revealed that CdSe NCs were more toxic to all three cell lines (HEK-293, MCF-7, and HeLa) than CdSe@CdS core–shell structures. Furthermore, a decrease in mitochondrial membrane potential and intracellular ROS production supported NCs inducing oxidative stress, which led to apoptosis via the mitochondria-mediated pathway. Increased cytochrome c levels, regulation of pro-apoptotic gene expression (e.g., p53, Bax), and down-regulation of Bcl-2 all suggested cellular apoptosis occurred via the intrinsic pathway. Significantly, at an equivalent dose of core–shell NCs, core-only NCs induced more oxidative stress, resulting in increased apoptosis. These findings shed light on the role of a CdS surface coating in reducing free radical release, decreasing cytotoxicity, and improving fluorescence, advancing the field of cell imaging.

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“…The addition of a different shell can achieve a reduction in oxidation [ 25 ]. Free radical production is an important factor that contributes to NCs’ toxicity [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Divergent Responses of Hydrophilic CdSe and CdSe@CdS Core–Shell Nanocrystals in Apoptosis and In Vitro Cancer Cell Imaging: A Comparative Analysis

Das,

Bhatt,

Parambil

et al. 2023

JFB

View full text Add to dashboard Cite

show abstract

Concise nanotherapeutic modality for cancer involving graphene oxide dots in conjunction with ascorbic acid

Abstract: Cancer cells tend to have higher intracellular reactive oxygen species (ROS) levels and are more vulnerable to ROS-generating therapies such as ascorbic acid (H2Asc) therapy, whose potency has been explored...

Cited by 1 publication

References 66 publications

Divergent Responses of Hydrophilic CdSe and CdSe@CdS Core–Shell Nanocrystals in Apoptosis and In Vitro Cancer Cell Imaging: A Comparative Analysis

Divergent Responses of Hydrophilic CdSe and CdSe@CdS Core–Shell Nanocrystals in Apoptosis and In Vitro Cancer Cell Imaging: A Comparative Analysis

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