Concise review: Harnessing iPSC-derived cells for ischemic heart disease treatment

Duan, Bin

doi:10.2478/jtim-2020-0004

Cited by 11 publications

(5 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability to develop patient-derived hiPSCs have opened new avenues to tailor personalized medicine approaches for numerous disorders and degenerative diseases [ 2 ]. Furthermore, the expandability and pluripotency of hiPSCs has been critical for their use in developing cell-based therapies for traumatic brain injury [ 3 , 4 ], spinal cord injury [ 5 , 6 ], myocardial infarction [ 7 , 8 ], acute kidney injury [ 9 ], and diabetes mellitus [ 10 , 11 ]. However, despite the improved functional outcomes observed in preclinical studies involving hiPSC-based cell therapy, several studies reported poor engraftment and long-term survival of the transplanted cells [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

Sridharan,

Dougherty,

Ahmed

et al. 2024

Stem Cell Res Ther

View full text Add to dashboard Cite

Background Human induced pluripotent stem cells (hiPSCs) and their differentiated cell types have a great potential for tissue repair and regeneration. While the primary focus of using hiPSCs has historically been to regenerate damaged tissue, emerging studies have shown a more potent effect of hiPSC-derived paracrine factors on tissue regeneration. However, the precise contents of the transplanted hiPSC-derived cell secretome are ambiguous. This is mainly due to the lack of tools to distinguish cell-specific secretome from host-derived proteins in a complex tissue microenvironment in vivo. Methods In this study, we present the generation and characterization of a novel hiPSC line, L274G-hiPSC, expressing the murine mutant methionyl-tRNA synthetase, L274GMmMetRS, which can be used for tracking the cell specific proteome via biorthogonal non-canonical amino acid tagging (BONCAT). We assessed the trilineage differentiation potential of the L274G-hiPSCs in vitro and in vivo. Furthermore, we assessed the cell-specific proteome labelling in the L274G-hiPSC derived cardiomyocytes (L274G-hiPSC-CMs) in vitro following co-culture with wild type human umbilical vein derived endothelial cells and in vivo post transplantation in murine hearts. Results We demonstrated that the L274G-hiPSCs exhibit typical hiPSC characteristics and that we can efficiently track the cell-specific proteome in their differentiated progenies belonging to the three germ lineages, including L274G-hiPSC-CMs. Finally, we demonstrated cell-specific BONCAT in transplanted L274G-hiPSC-CMs. Conclusion The novel L274G-hiPSC line can be used to study the cell-specific proteome of hiPSCs in vitro and in vivo, to delineate mechanisms underlying hiPSC-based cell therapies for a variety of regenerative medicine applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

Sridharan,

Dougherty,

Ahmed

et al. 2024

Stem Cell Res Ther

View full text Add to dashboard Cite

show abstract

“…Glutaminase 2 (GLS2) can cause ROS accumulation through mitochondria by accelerating glutamate formation ( Matés et al, 2020 ). After myocardial infarction, cardiomyocytes often undergo cell death and pathological remodeling, which easily lead to heart failure ( Duan, 2020 ). Zhou et al have suggested that miR-190a-5p inhibits cardiomyocyte ferroptosis by inhibiting GLS2 and decreases the levels of ROS, MDA, and Fe 2+ in H9c2 cells, thus playing a protective role in myocardial infarction ( Zhou et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The role of microRNAs in ferroptosis

Guo

Zhang²,

Liu³

2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Ferroptosis is a newly discovered type of programmed cell death, which is closely related to the imbalance of iron metabolism and oxidative stress. Ferroptosis has become an important research topic in the fields of cardiomyopathy, tumors, neuronal injury disorders, and ischemia perfusion disorders. As an important part of non-coding RNA, microRNAs regulate various metabolic pathways in the human body at the post-transcriptional level and play a crucial role in the occurrence and development of many diseases. The present review introduces the mechanisms of ferroptosis and describes the relevant pathways by which microRNAs affect cardiomyopathy, tumors, neuronal injury disorders and ischemia perfusion disorders through regulating ferroptosis. In addition, it provides important insights into ferroptosis-related microRNAs, aiming to uncover new methods for treatment of the above diseases, and discusses new ideas for the implementation of possible microRNA-based ferroptosis-targeted therapies in the future.

show abstract

“…In the complex pathologic process of MIRI, various stages are involved, involving platelet activation, oxidative stress, endothelial dysfunction, and inflammatory response (5). Therapeutic strategies for MIRI mainly consist of non-pharmacological interventions (ischemic preconditioning, ischemic postconditioning, and remote ischemic preconditioning), pharmacological interventions (cyclosporine A, atrial natriuretic peptide, and glucagonlike peptide 1) (6,7), and even implementation of humaninduced pluripotent cells (8). Although cell and animal experiments have confirmed the effectiveness of the therapies, the results of clinical trials have been disappointing.…”

Section: Introductionmentioning

confidence: 99%

Downregulated ALKBH5 contributes to myocardial ischemia/reperfusion injury by increasing m6A modification of Trio mRNA

Li¹,

Chen²,

Zhao³

et al. 2022

Ann Transl Med

View full text Add to dashboard Cite

Background: The modification of N 6 -methyladenosine (m 6 A) is a dynamic and reversible course that might play a role in cardiovascular disease. However, the mechanisms of m 6 A modification in myocardial ischemia/reperfusion injury (MIRI) remain unclear.Methods: A mouse model of MIRI and a cell model of oxygen-glucose deprivation/reperfusion (OGD/ R) HL-1 cells were employed. In an in vivo study, the total RNA m 6 A modification levels were determined by dot blot, and the key genes related to m 6 A modification were screened by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In an in vitro study, the effects of AlkB homolog 5 (ALKBH5), an RNA demethylase, on cell proliferation, cell injury, and apoptosis were detected by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, lactate dehydrogenase (LDH) and cardiac troponin-I (cTnI) levels, and flow cytometry. Besides, the m 6 A modification-changed and differentially expressed messenger RNA (mRNA) were determined by methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) in ALKBH5overexpressed HL-1 cells. Finally, the mRNA levels of the promising targeted gene were examined by RT-qPCR and its m 6 A modification levels were examined by MeRIP-qPCR.Results: Our results showed that RNA m 6 A modification was involved in MIRI, in which ALKBH5 was downregulated. Functionally, by overexpressing or silencing ALKBH5 in experimental cells, we verified its protective properties on cell proliferation, cell injury, and apoptosis in the process of MIRI. Besides, we provided a mass of latent different mRNAs with m 6 A modification variation in ALKBH5-overexpressed HL-1 cells. Mechanistically, we further screened the most potential targeted mRNAs and suggested that triple functional domain (Trio) mRNA could be upregulated by ALKBH5 by reducing m 6 A level of Trio.

show abstract

Concise review: Harnessing iPSC-derived cells for ischemic heart disease treatment

Cited by 11 publications

References 70 publications

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

Bioorthogonal non-canonical amino acid tagging to track transplanted human induced pluripotent stem cell-specific proteome

The role of microRNAs in ferroptosis

Downregulated ALKBH5 contributes to myocardial ischemia/reperfusion injury by increasing m6A modification of Trio mRNA

Contact Info

Product

Resources

About