Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing

Chamberlain, Giselle; Fox, James M.; Ashton, Brian A.; Middleton, Jim

doi:10.1634/stemcells.2007-0197

Cited by 2,152 publications

(1,777 citation statements)

References 126 publications

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“…[24] However, they are also capable of differentiating into nonmesenchymal cells like neural cells [25] under suitable conditions. Earlier, differentiation of hMSCs was tried and carried out by the addition of various growth factors in the culture media at different stages of growth or maturation.…”

Section: Growth Factor Delivery Via A8 Hydrogel Formed In Presence Anmentioning

confidence: 99%

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Das

Kumar

Jha

et al. 2017

Adv Healthcare Materials

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promoting regeneration. [3] Hydrogels present a unique microstructure to the encapsulated cells influencing cell signaling and hence cellular fate. Peptide hydrogels are a class of physical hydrogels where the component peptides self-assemble noncovalently to form an ordered nanostructure [4] and eventually supramolecular networks, which entrap water for gelation. [5] Recently with the advent of protein engineering, relatively simple peptide based hydrogels have gained increasing attention in biomaterial development. [6] The major advantage of employing short peptides as hydrogelators is that their properties could be easily tuned and controlled via side chain modification and chemical alteration to achieve the desired functionality. [7] Moreover, peptide hydrogels are also advantageous as they have less potential for inflammation and also have biodegradable components [8] compared to polymer based hydrogels. Recently, we have shown the application of another class of hydrogels based on "amyloid fibrils" for possible application in 2D, 3D cell culture, and stem cell differentiation. [2a,b] Amyloids are highly stable protein/peptide aggregates with cross-β-sheet rich structure. [9] Although amyloids are historically associated with various human diseases, recent discoveries have suggested functional roles of amyloids that help organisms survival rather causing diseases. [10] Also, studies have shown that amyloid fibrils are rather benign or less toxic species compared to soluble oligomers. [11] Due to higher order structure, amyloid possesses unique stability against various harsh environmental conditions such as wide ranges of pHs, temperature, and proteases, which makes amyloids attractive for designing biomaterials for tissue engineering as well as various nanotechnological applications. [2h,12] Recently, a number of studies also indicate that amyloids are unique cell adhesive material that promotes adhesion of cells without the presence of tripeptide motif Arg-Gly-Asp (RGD) motif. [13] Our recent study has also shown that amyloid based hydrogels could be suitably used as implantable materials for tissue engineering, owing to their noninflammatory property along with ease of delivery with minimally invasive surgery. [2b] In a natural tissue environment, extra cellular matrix (ECM) has a favorable nanotopography [14] for cells and contains large depots of growth factors that affect the physiological functions of the cells. [3c] In an attempt to mimic such a natural niche to provide better stem cell differentiation, presence and controlled exposure of various growth factors are Amyloid based hydrogels can mimic the extracellular matrix and serve as matrices for tissue engineering both in vitro and in vivo. A pH responsive selfassembled amyloid hydrogel system is used to encapsulate various growth factors for driving stem cell differentiation toward neuronal lineage. Diffusion studies with fluorescence recovery after photobleaching and bulk release with the model protein fluorescein isothiocyanate-bovin...

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Section: Growth Factor Delivery Via A8 Hydrogel Formed In Presence Anmentioning

confidence: 99%

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Das

Kumar

Jha

et al. 2017

Adv Healthcare Materials

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“…5 MSCs can migrate to sites of tissue damage, 6,7 where they are activated by inflammatory cytokines (such as IFN-c) and several chemokines, which can recruit or suppress lymphocytes. [8][9][10][11] Interleukin-1b (IL-1b) is an important inflammatory factor that is expressed abundantly during the active phase of UC. [12][13][14] The UC mice induced by DSS treatment exhibit many symptoms similar to human UC, such as diarrhea, bloody feces, body weight loss, mucosal ulceration and shortening of the large intestine.…”

Section: Introductionmentioning

confidence: 99%

Pre-treatment with IL-1β enhances the efficacy of MSC transplantation in DSS-induced colitis

et al. 2012

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Mesenchymal stem cells (MSCs) have been used experimentally for treating inflammatory disorders, partly due to their immunosuppressive properties. Although interleukin-1b (IL-1b) is one of the most important inflammatory mediators, growing evidence indicates that IL-1b signaling elicits the immunosuppressive properties of MSCs. However, it remains unclear how IL-1b signaling accomplishes this activity. Here, we focus on the therapeutic efficacy of IL-1b-primed MSCs in the dextran sulfate sodium (DSS)-induced colitis model, in addition to the underlining mechanisms. We first found that IL-1b-primed MSCs, without any observable phenotype change in vitro, significantly attenuated the development of DSS-induced murine colitis. Moreover, IL-1b-primed MSCs modulated the balance of immune cells in the spleen and the mesenteric lymph nodes (MLNs) through elevating cyclooxygenase-2 (COX-2), IL-6 and IL-8 expression and influencing the polarization of peritoneal macrophages. Importantly, IL-1b-primed MSCs possessed an enhanced ability to migrate to the inflammatory site of the gut via upregulation of chemokine receptor type 4 (CXCR4) expression. In summary, IL-1b-primed MSCs have improved efficacy in treating DSS-induced colitis, which at least partly depends on their increased immunosuppressive capacities and enhanced migration ability.

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“…24 Other studies have shown that MSC transplantation into fetal or neonatal animals have resulted in the engraftment to different tissues without provoking an immune response. 25,26 Even though most of the MSC get trapped in the lungs after intravenous injection, evidence shows that MSC are capable of homing to the injured tissue after intravenous injection. Cultured rat and human MSC have been shown to migrate into the sites of brain injury after cerebral ischemia when transplanted intravenously in rats.…”

Section: Potential Clinical Application Of Mscmentioning

confidence: 99%

Therapeutic potential of genetically modified mesenchymal stem cells

2008

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Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing

Abstract: ABSTRACT

Cited by 2,152 publications

References 126 publications

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Controlled Exposure of Bioactive Growth Factor in 3D Amyloid Hydrogel for Stem Cells Differentiation

Pre-treatment with IL-1β enhances the efficacy of MSC transplantation in DSS-induced colitis

Therapeutic potential of genetically modified mesenchymal stem cells

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