Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

Witjas, Franca M.R.; Berg, Bernard M. van den; Berg, Cathelijne W. van den; Engelse, Marten A.; Rabelink, Ton J.

doi:10.1002/sctm.18-0155

Cited by 68 publications

(56 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This further underscores the relevance of the endothelial ECM as an organotypic signaling platform involved in angiocrine signaling and tissue homeostasis. 29 It may also point to an increased dependency of these specific vascular beds on Ang1-Tie2 signaling for their function. 30 Indeed, glomerular endothelium has a high expression of the Tie2 receptor relative to other vascular beds.…”

Section: Discussionmentioning

confidence: 99%

Glomerular Function and Structural Integrity Depend on Hyaluronan Synthesis by Glomerular Endothelium

Berg¹,

Wang²,

Boels³

et al. 2019

JASN

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Glomerular Function and Structural Integrity Depend on Hyaluronan Synthesis by Glomerular Endothelium

Berg¹,

Wang²,

Boels³

et al. 2019

JASN

Self Cite

View full text Add to dashboard Cite

show abstract

“…The stem cell niche is comprised of two main factors: 1) differentiated cells, often important for producing signals such as growth factors; and 2) the extracellular matrix (ECM), the network of insoluble proteins that provides structure to the intercellular space [3][4][5]. A growing body of evidence supports the ECM as an important regulator of stem cell behaviors, including division, quiescence, and differentiation [3,[6][7][8]. At the tissue level, the ECM also provides a physical barrier that prevents neoplastic stem cells from leaving their compartment and invading into other tissues, thereby slowing tumour progression and metastasis [4,9,10].…”

Section: Introductionmentioning

confidence: 99%

Planarian EGF repeat-containing genes megf6 and hemicentin are required to restrict the stem cell compartment

2020

View full text Add to dashboard Cite

The extracellular matrix (ECM) is important for maintaining the boundaries between tissues. This role is particularly critical in the stem cell niche, as pre-neoplastic or cancerous stem cells must pass these boundaries in order to invade into the surrounding tissue. Here, we examine the role of the ECM as a regulator of the stem cell compartment in the planarian Schmidtea mediterranea, a highly regenerative, long-lived organism with a large population of adult stem cells. We identify two EGF repeat-containing genes, megf6 and hemicentin, with identical knockdown phenotypes. We find that megf6 and hemicentin are needed to maintain the structure of the basal lamina, and in the absence of either gene, pluripotent stem cells migrate ectopically outside of their compartment and hyper-proliferate, causing lesions in the body wall muscle. These muscle lesions and ectopic stem cells are also associated with ectopic gut branches, which protrude from the normal gut towards the dorsal side of the animal. Interestingly, both megf6 and hemicentin knockdown worms are capable of regenerating tissue free of both muscle lesions and ectopic cells, indicating that these genes are dispensable for regeneration. These results provide insight into the role of planarian ECM in restricting the stem cell compartment, and suggest that signals within the compartment may act to suppress stem cell hyperproliferation. expressed in different cell populations, we find that both are required to maintain the epithelial basal lamina. In the absence of either gene, stem cells can escape their compartment and migrate towards the skin of the animal, where they divide at an accelerated rate and cause lesions in the muscle. These results show that the extracellular matrix plays a role in limiting the boundaries of the stem cell compartment. megf6 and hemicentin restrict the planarian stem cell niche PLOS Genetics | https://doi.

show abstract

“…18 Physical contact is essential for cells bioactivities such as proliferation, cell migration, and differentiation. 19 These events were promptly facilitated by neighboring scaffolds. 20 Even, the type of ECM participates in the regulation of cell expansion, migration, and survival.…”

Section: Microencapsulation Increased Emt Ratementioning

confidence: 99%

Hollow Alginate-Poly-L-Lysine-Alginate Microspheres Promoted an Epithelial-Mesenchymal Transition in Human Colon Adenocarcinoma Cells

et al. 2019

View full text Add to dashboard Cite

Purpose: Today, there is an urgent need to develop a three-dimentional culture systems mimicking native in vivo condition in order to screen potency of drugs and possibly any genetic alterations in tumor cells. Due to the existence of limitations in animal models, the development of three dimensional systems is highly recommended. To this end, we encapsulated human colon adenocarcinoma cell line HT29 with alginate-poly-L-lysine (Alg-PLL) microspheres and the rate of epithelial-mesenchymal transition was monitored. Methods: Cells were randomly divided into three groups; control, alginate and Alg-PLL. To encapsulate cells, we mixed HT-29 cells (1 × 106 ) with 1 mL of 0.05% PLL and 1% Alg mixture and electrosprayed into CaCl2 solution by using a high-voltage power. Cells from all groups were maintained at 37˚C in a humidified atmosphere containing 5% CO2 for 7 days. Cell viability was assessed by MTT assay. To monitor the stemness feature, we measured the transcription of genes such as Snail, Zeb, and Vimentin by using real-time PCR analysis. Results: Addition of PLL to Alg in a hallowed state increased the cell survival rate compared to the control and Alg groups (P<0.05). Cells inside Alg-PLL tended to form microcellular aggregates while in Alg microspheres an even distribution of HT-29 cells was found. Real-time PCR analysis showed the up-regulation of Snail, Zeb, and Vimentin in Alg-PLL microspheres compared to the other groups, showing the acquisition of stemness feature (P<0.05). Conclusion: This study showed that hallow Alg-PLL microspheres increased the epithelialmesenchymal transition rate after 7 days in in vitro condition. Such approaches could be touted as appropriate in vitro models for drug screening.

show abstract

Concise Review: The Endothelial Cell Extracellular Matrix Regulates Tissue Homeostasis and Repair

Cited by 68 publications

References 64 publications

Glomerular Function and Structural Integrity Depend on Hyaluronan Synthesis by Glomerular Endothelium

Glomerular Function and Structural Integrity Depend on Hyaluronan Synthesis by Glomerular Endothelium

Planarian EGF repeat-containing genes megf6 and hemicentin are required to restrict the stem cell compartment

Hollow Alginate-Poly-L-Lysine-Alginate Microspheres Promoted an Epithelial-Mesenchymal Transition in Human Colon Adenocarcinoma Cells

Contact Info

Product

Resources

About