Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

Bussotti, Filippo; Pollastrini, Martina; Cascio, Chiara; Desotgiu, Rosanna; Gerosa, Giacomo; Marzuoli, Riccardo; Nali, Cristina; Lorenzini, Giacomo; Pellegrini, Elisa; Carucci, Maria Giovanna; Salvatori, Elisabetta; Fusaro, Lina; Piccotto, Massimo; Malaspina, Paola; Manfredi, Alice; Roccotello, Enrica; Toscano, Stefania; Gottardini, E.; Cristofori, Alessandro; Fini, Alessio; Weber, Daniel; Baldassarre, V.; Barbanti, Lorenzo; Monti, Andrea; Strasser, Reto J.

doi:10.1016/j.envexpbot.2010.10.023

Cited by 23 publications

(12 citation statements)

References 10 publications

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“…These strategies are reflected on ChlF parameters and the MR/MR 0 signal appears to be especially sensitive. Finally, the fourth cluster, including experiments 7 and 9 that were realized with a different M-PEA unit, suggests the lack of comparability of the results obtained with different M-PEA units, as previously highlighted for PF measurements acquired with Handy-PEA instruments (Bussotti et al, 2011b). Finally, the species-specific clustering of the experiments based on DF signal also supports the need to design a species-specific approach for detecting plant stress response, as already suggested by Kalaji et al (2014a).…”

Section: Discussionmentioning

confidence: 62%

“…This increase in V I is usually connected with a reduced energy demand from the Calvin cycle, and it is known as an early response to different stress factors, such as O 3 (Bussotti et al, 2011a,b;Desotgiu et al, 2012), salt (Mereu et al, 2011) and drought (Oukarroum et al, 2007(Oukarroum et al, , 2009Pollastrini et al, 2014a). Moreover, in the experiments 1e5, a typical down regulation of PSII photochemistry was also evident at the single turnover region of the fluorescence transient, with the reduction of F V /F M , and the increase in V J, indicating accumulation of Q A À (Strasser et al, 2004;Bussotti et al, 2011b).…”

Section: Discussionmentioning

confidence: 88%

“…The JIP-test was applied to the PF transients, and the following parameters were derived from each curve: F 0 , the initial fluorescence intensity measured by the MPEA at 20 ms; F M , the maximal measured fluorescence intensity; F V /F M ¼ (F M À F 0 )/F M , the maximum quantum yield of PSII; V J , the relative variable fluorescence at 3 ms, calculated as V J ¼ (F J À F 0 )/(F M À F 0 ); V I, the relative variable fluorescence at 30 ms, Michael and Strasser, 2008;Bussotti et al, 2011b).…”

Section: Data Processingmentioning

confidence: 99%

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Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Salvatori

Fusaro

Gottardini

et al. 2014

Plant Physiology and Biochemistry

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 88%

Section: Data Processingmentioning

confidence: 99%

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Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Salvatori

Fusaro

Gottardini

et al. 2014

Plant Physiology and Biochemistry

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“…An example of the phenomenon, described above, is a study in which the same leaves were measured with different HandyPEA instruments (Bussotti et al 2011a ) calibrated with identical settings (lamp intensity = 3,000 μmol photons m −2 s −1 , time = 1 s, gain = 1). Both original and normalized transient curves were compared.…”

Section: Question 27 Can Measurements Made With Different Instrumentmentioning

confidence: 99%

“…The parameter F O / F M (parameter which is sensitive to changes in heat dissipation in the PSII antenna), as well as the normalized steps of OJIP transients—J and I (fluorescence intensities at 2–3 and 30 ms, respectively)—showed very little variability when comparing the measurements of the different instruments with a coefficient of variation (CV = SD/Mean) ranging from 3 to 5 %. The parameter PIabs, which consists of the product of a parameter sensitive to the effective antenna size, a parameter based on the maximum quantum yield of PSII, and a parameter sensitive to changes in the relative position of F J (see Question 19) showed a very high variability among instruments (PIabs showed a CV = 30 %; Bussotti et al 2011a ). The high intrinsic variability of PIabs between instruments is due to the fact that this parameter is sensitive to the initial slope of the fluorescence rise and the relative position of the J-step, two factors that are both relatively sensitive to the light intensity of the beam.…”

Section: Question 27 Can Measurements Made With Different Instrumentmentioning

confidence: 99%

Frequently asked questions about in vivo chlorophyll fluorescence: practical issues

Schansker²,

et al. 2014

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The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

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Experimental in vivo measurements of light emission in plants: a perspective dedicated to David Walker

et al. 2012

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This review is dedicated to David Walker (1928-2012), a pioneer in the field of photosynthesis and chlorophyll fluorescence. We begin this review by presenting the history of light emission studies, from the ancient times. Light emission from plants is of several kinds: prompt fluorescence (PF), delayed fluorescence (DF), thermoluminescence, and phosphorescence. In this article, we focus on PF and DF. Chlorophyll a fluorescence measurements have been used for more than 80 years to study photosynthesis, particularly photosystem II (PSII) since 1961. This technique has become a regular trusted probe in agricultural and biological research. Many measured and calculated parameters are good biomarkers or indicators of plant tolerance to different abiotic and biotic stressors. This would never have been possible without the rapid development of new fluorometers. To date, most of these instruments are based mainly on two different operational principles for measuring variable chlorophyll a fluorescence: (1) a PF signal produced following a pulse-amplitude-modulated excitation and (2) a PF signal emitted during a strong continuous actinic excitation. In addition to fluorometers, other instruments have been developed to measure additional signals, such as DF, originating from PSII, and light-induced absorbance changes due to the photooxidation of P700, from PSI, measured as the absorption decrease (photobleaching) at about 705 nm, or increase at 820 nm. In this review, the technical and theoretical basis of newly developed instruments, allowing for simultaneous measurement of the PF and the DF as well as other parameters is discussed. Special emphasis has been given to a description of comparative measurements on PF and DF. However, DF has been discussed in greater details, since it is much less used and less known than PF, but has a great potential to provide useful qualitative new information on the back reactions of PSII electron transfer. A review concerning the history of fluorometers is also presented.

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Conclusive remarks. Reliability and comparability of chlorophyll fluorescence data from several field teams

Cited by 23 publications

References 10 publications

Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

Frequently asked questions about in vivo chlorophyll fluorescence: practical issues

Experimental in vivo measurements of light emission in plants: a perspective dedicated to David Walker

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