Concomitant augmentation of CD4+CD29+ helper inducer and diminution of CD4+CD45RA+ suppressor inducer subset in patients infected with human T cell lymphotropic virus types I or II

Lal, Renu B.; Rudolph, Donna L.; Schmid, Daniela; Lairmore, Michael Dale

doi:10.1111/j.1365-2249.1992.tb02990.x

Cited by 7 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because p300 is limited in cells, the expression of p300 in long-lived lymphocytes, such as memory T cells, would be particularly important for their survival. Interestingly, HTLV-1 has been demonstrated to target memory T cells (Yoshie et al, 2002;Lal et al, 1992). We demonstrate that ER localization of p12 I is required for its ability to increase p300.…”

Section: Discussionmentioning

confidence: 78%

Calcium-dependent enhancement of transcription of p300 by human T-lymphotropic type 1 p12I

et al. 2006

View full text Add to dashboard Cite

Human T-lymphotropic virus type 1 (HTLV-1) p12I localizes to the endoplasmic reticulum and Golgi causing sustained release of calcium, T cell activation, and enhanced expression of several calcium-regulated genes. In recent microarray studies, p300 mRNA was increased in T cells expressing p12I. The co-activator p300 is a key regulator of cellular and viral transcription; however, factors that influence its transcriptional regulation are less well studied. We hypothesized that the transcription of p300 is calcium dependent and that sustained low magnitude increases in intracellular calcium may enhance the transcription of p300. Herein, we report enhanced expression of p300 in T cells by p12I in a calcium-dependent, but calcineurin-independent manner. Sustained low magnitude calcium release induced by ionomycin in T cells was sufficient to increased mRNA and protein levels of p300 resulting in enhanced transcription from a p300-dependent promoter. Promoter analysis of the p300 gene was used to predict calcium-responsive transcription factor binding sites. Using mutant forms of p12I, we demonstrate that ER localization of the viral protein is required to increase p300. In addition, p12I reversed the repression of HTLV-1 LTR-driven transcription by HTLV-1 p30II, a p300-binding protein. HTLV-1 p12I-mediated enhancement of p300 expression represents a novel mechanism of regulation of cellular gene expression by viral proteins. By targeting a ubiquitous second messenger such as calcium, HTLV-1 p12I may regulate the expression of the cellular transcriptional co-activator p300 to modulate viral gene expression and promote lymphocyte survival.

show abstract

Section: Discussionmentioning

confidence: 78%

Calcium-dependent enhancement of transcription of p300 by human T-lymphotropic type 1 p12I

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Têm sido cada vez mais frequentes as investigações que avaliam se a associação destes dois tipos de retrovírus poderia resultar em aceleração da evolução para AIDS. Os resultados até o momento são controversos(230)(231)(232)(233).…”

unclassified

Infecção pelos retrovírus HIV-1, HTLV-I e HTLV-II na população feminina da Penitenciária do Estado de São Paulo: prevalência, fatores de risco e conhecimento desse risco

Ferreira¹

View full text Add to dashboard Cite

LA V -Vírus associado a linfoadenopatia, "Linphoadenopaty associated virus" MMTV -Vírus de tumor mamario de camundongos, "Mouse mammary tumor vírus" MPMV -Vírus Mason-Pfizer de macacos, "Mason-Pfizer monkey vírus" MS -Ministério da Saúde MuLV -Vírus da leucemia murina de Moloney, "Moloney murine leukemia virus" MuSV -Vírus do Sarcoma murino de Moloney, "Moloney murine sarcoma virus" OMSIWHO -Organização Mundial da Saúde, "World Health Organization" OR -"Odds Ratio" peR -Reação em cadeia da polimerase, "Polymerase Chain Reaction" RNA -Ácido ribonucléico, "Ribonucleic acid" RSV/ASV -Vírus de sarcoma de aves, "Rous sarcoma vírus /Avian sarcoma virus" SiSFV -Vírus foamy dos símios, "Simiam foamy vírus" SIV -Vírus da imunodeficiência simiam, "Simiam immunodeficiency virus" SRV -Retrovirus dos símios, "Simiam retro vírus" SSA V -Vírus associado ao sarcoma dos símios, "Simiam sarcoma associated vírus" STL V -Vírus da leucemia de células T dos símios, "Simiam T -celIleukemia virus"

show abstract

Phenotypic expression of integrin membrane receptors on spontaneously proliferating CD8 cells in human T-lymphotropic virus type II (HTLV-II)-infected individuals

Lal¹,

Rudolph²,

Rowe³

et al. 1992

J Clin Immunol

View full text Add to dashboard Cite

Spontaneous lymphocyte proliferation in the absence of exogenous stimulators was examined in asymptomatic HTLV-II-seropositive (n = 12) and seronegative individuals (n = 16). Mean spontaneous lymphocytic proliferation significantly increased on day 8 postculture in HTLV-II-infected individuals (5762 +/- 899 cpm) compared with normal controls (2034 +/- 925 cpm, P less than 0.01). The proliferating cells in infected individuals were predominantly T cells; neither B cells nor monocytes demonstrated any proliferation. Phenotypic analysis of cultured cells from individuals with HTLV-II infection demonstrated differential expression of integrin molecules as defined by anti-CD29 and anti-S6F1 (42.8 +/- 4.2 and 39.6 +/- 5.9%, respectively) on CD8 cells, as compared with day 0 peripheral blood mononuclear cells (PBMC) from infected individuals (19.7 +/- 3.5 and 19.9 +/- 1.9%, respectively) or normal controls (12.9 +/- 3.1 and 11.5 +/- 2.5%, respectively; P less than 0.001 for both comparisons). These CD8+ cells did not express CD16 or CD11b. The culture supernatants derived from the spontaneously proliferating cells had significantly increased levels of sCD8 and sCD25 (765 +/- 180 and 1805 +/- 320 U/ml, respectively) compared with those from normal controls (222 +/- 120 and 305 +/- 90 U/ml, respectively; P less than 0.01). Furthermore, culture supernatants derived from spontaneously proliferating PBMC from HTLV-II-infected individuals had no detectable levels of HTLV antigen and did not stimulate proliferation of PBMC from normal donors. These results suggest that the spontaneous proliferation in HTLV-II asymptomatic carriers is due to expansion of CD8 cells expressing integrin receptors which may serve as costimulatory molecules for their activation.

show abstract

Concomitant augmentation of CD4+CD29+ helper inducer and diminution of CD4+CD45RA+ suppressor inducer subset in patients infected with human T cell lymphotropic virus types I or II

Cited by 7 publications

References 34 publications

Calcium-dependent enhancement of transcription of p300 by human T-lymphotropic type 1 p12I

Calcium-dependent enhancement of transcription of p300 by human T-lymphotropic type 1 p12I

Infecção pelos retrovírus HIV-1, HTLV-I e HTLV-II na população feminina da Penitenciária do Estado de São Paulo: prevalência, fatores de risco e conhecimento desse risco

Phenotypic expression of integrin membrane receptors on spontaneously proliferating CD8 cells in human T-lymphotropic virus type II (HTLV-II)-infected individuals

Contact Info

Product

Resources

About