Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon

Tchetgna, Huguette Simo; Yousseu, Francine Sado; Kamgang, Basile; Tedjou, Armel N.; McCall, Philip; Wondji, Charles S.

doi:10.1101/2021.05.10.21256254

Cited by 6 publications

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Prospective case-control study of dengue infection in some malaria and non-malaria patients consulting at the Bertoua Regional Hospital, East-Cameroon

Galani

Ayangma

Wouatedem

et al. 2021

Preprint

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In Cameroon, recent studies have documented several cases of dengue infection in some urban areas, but dengue-malaria coinfection malaria was little investigated, especially in the East region. From the 6th July to the 4th September 2020, a case-control study including 50 cases (malaria-positive subjects) and 90 controls (non-malaria subjects) was carried out at the Bertoua Regional Hospital. Participants were prospectively enrolled and administered with a questionnaire to record information such as age, sex, dwelling place, dengue knowledge, and the environment's quality. Blood specimens were then collected and screened for dengue infection using the NS1/IgG/IgM rapid diagnostic tests and hematological parameters were measured using a MINDRAY-type hemacytometer. Of the malaria patients, 14% (7/50) were tested positive for dengue fever against 66.66% for the controls (60/90). Most malaria patients had a secondary dengue infection (57.14%, 4/7) while most of the non-malaria patients faced a primary infection (61.66%, 37/60). In both groups, women were more exposed than men to dengue and there was a significant association between the dwelling place and dengue seropositivity. Moreover, young subjects <16 years old were significantly more associated with dengue than 16-30 years old (OR=16.24, P=0.042 for cases vs, OR=21, P=0.0001 for controls). The analysis of hematological parameters showed a significant decrease (P<0.001) in platelets in cases compared to control. These results suggest that dengue fewer targets malaria- than non-malaria patients with different serological characteristics in Bertoua city. However, co-infected patients demonstrated a greater clinical vulnerability than monoinfected patients, urging the need for epidemiological surveillance.

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Prospective case-control study of dengue infection in some malaria and non-malaria patients consulting at the Bertoua Regional Hospital, East-Cameroon

Galani

Ayangma

Wouatedem

et al. 2021

Preprint

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Seroprevalence of Dengue and Chikungunya Virus Infections in Children Living in Sub-Saharan Africa: Systematic Review and Meta-Analysis

Kayange,

Hau,

Pain

et al. 2023

Children

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Dengue and chikungunya viruses are frequent causes of malarial-like febrile illness in children. The rapid increase in virus transmission by mosquitoes is a global health concern. This is the first systematic review and meta-analysis of the childhood prevalence of dengue and chikungunya in Sub-Saharan Africa (SSA). A comprehensive search of the MEDLINE (Ovid), Embase (Ovid), and Cochrane Library (Wiley) databases was conducted on 28 June 2019, and updated on 12 February 2022. The search strategy was designed to retrieve all articles pertaining to arboviruses in SSA children using both controlled vocabulary and keywords. The pooled (weighted) proportion of dengue and chikungunya was estimated using a random effect model. The overall pooled prevalence of dengue and chikungunya in SSA children was estimated to be 16% and 7%, respectively. Prevalence was slightly lower during the period 2010–2020 compared to 2000–2009. The study design varied depending on the healthcare facility reporting the disease outbreak. Importantly, laboratory methods used to detect arbovirus infections differed. The present review documents the prevalence of dengue and chikungunya in pediatric patients throughout SSA. The results provide unprecedented insight into the transmission of dengue and chikungunya viruses among these children and highlight the need for enhanced surveillance and controlled methodology.

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Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021

et al. 2022

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Senegal is hyperendemic for dengue. Since 2017, outbreaks have been noticed annually in many regions around the country, marked by the co-circulation of DENV1-3. On 8 October 2021, a Dengue virus outbreak in the Rosso health post (sentinel site of the syndromic surveillance network) located in the north of the country was notified to the WHO Collaborating Center for arboviruses and hemorrhagic fever viruses at Institut Pasteur de Dakar. A multidisciplinary team was then sent for epidemiological and virologic investigations. This study describes the results from investigations during an outbreak in Senegal using a rapid diagnostic test (RDT) for the combined detection of dengue virus non-structural protein 1 (NS1) and IgM/IgG. For confirmation, samples were also tested by real-time RT-PCR and IgM ELISA at the reference lab in Dakar. qRT-PCR positive samples were subjected to whole genome sequencing using nanopore technology. Virologic analysis scored 102 positives cases (RT-PCR, NS1 antigen detection and/or IgM) out of 173 enrolled patients; interestingly, virus serotyping showed that the outbreak was caused by the DENV-1, a serotype different from DENV-2 involved during the outbreak in Rosso three years earlier, indicating a serotype replacement. Nearly all field-tested NS1 positives samples were confirmed by qRT-PCR with a concordance of 92.3%. Whole genome sequencing and phylogenetic analysis of strains suggested a re-introduction in Rosso of a DENV-1 strain different to the one responsible for the outbreak in the Louga area five years before. Findings call for improved dengue virus surveillance in Senegal, with a wide deployment of DENV antigenic tests, which allow easy on-site diagnosis of suspected cases and early detection of outbreaks. This work highlights the need for continuous monitoring of circulating serotypes which is crucial for a better understanding of viral epidemiology around the country.

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Concurrent circulation of dengue serotype 1, 2 and 3 among acute febrile patients in Cameroon

Cited by 6 publications

References 50 publications

Prospective case-control study of dengue infection in some malaria and non-malaria patients consulting at the Bertoua Regional Hospital, East-Cameroon

Prospective case-control study of dengue infection in some malaria and non-malaria patients consulting at the Bertoua Regional Hospital, East-Cameroon

Seroprevalence of Dengue and Chikungunya Virus Infections in Children Living in Sub-Saharan Africa: Systematic Review and Meta-Analysis

Analysis of a Dengue Virus Outbreak in Rosso, Senegal 2021

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