Concurrent exercise training and Murf-l and Atrogin-1 gene expression in the vastus lateralis muscle of male Wistar rats

Moradi, Yashar; Zehsaz, Farzad; Nourazar, Mir Alireza

doi:10.1016/j.apunsm.2020.02.001

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Exercise increased the mRNA expressions of Atrogin-1 and Murf1 . Similar results have been observed in rats [ 131 ]. Exercise has also been described to upregulate apoptotic pathways within the muscle [ 132 , 133 ].…”

Section: Discussionsupporting

confidence: 91%

Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice

et al. 2022

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Obesity and aging promote chronic low-grade systemic inflammation. The aim of the study was to analyze the effects of long-term physical exercise and/or omega-3 fatty acid Docosahexaenoic acid (DHA) supplementation on genes or proteins related to muscle metabolism, inflammation, muscle damage/regeneration and myokine expression in aged and obese mice. Two-month-old C57BL/6J female mice received a control or a high-fat diet for 4 months. Then, the diet-induced obese (DIO) mice were distributed into four groups: DIO, DIO + DHA, DIO + EX (treadmill training) and DIO + DHA + EX up to 18 months. Mice fed a control diet were sacrificed at 2, 6 and 18 months. Aging increased the mRNA expression of Tnf-α and decreased the expression of genes related to glucose uptake (Glut1, Glut4), muscle atrophy (Murf1, Atrogin-1, Cas-9) and myokines (Metrnl, Il-6). In aged DIO mice, exercise restored several of these changes. It increased the expression of genes related to glucose uptake (Glut1, Glut4), fatty acid oxidation (Cpt1b, Acox), myokine expression (Fndc5, Il-6) and protein turnover, decreased Tnf-α expression and increased p-AKT/AKT ratio. No additional effects were observed when combining exercise and DHA. These data suggest the effectiveness of long-term training to prevent the deleterious effects of aging and obesity on muscle dysfunction.

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Section: Discussionsupporting

confidence: 91%

Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice

et al. 2022

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“…On the other hand, no significant differences were observed in signaling pathway related to muscle synthesis and atrophy between the untreated group and the stapled group. Several papers have shown that signaling pathway related to muscle mass may increase or decrease during treadmill exercise depending on the experimental conditions [36][37][38][39][40]. Studies conducted under experimental conditions similar to the present study, including treadmill exercise and time of dissection, have reported an increase in MuRF1 and mTOR associated with muscle atrophy after treadmill exercise [41,42].…”

Section: Discussionsupporting

confidence: 59%

Anti-muscle Atrophy Effects ofLactobacillus reuteriATG-F4 and Its Impact on Intestinal Microbiota and Metabolites: Implications for Prophylaxis and Therapy

Lee¹,

Lee²,

Park³

et al. 2023

Preprint

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Lactobacillus reuteri ATG-F4, human gut-derived bacteria, was orally administrated in a model of hindlimb immobilization and confirmed the muscular performance, muscle mass and mechanism on anti-atrophy study. Concomitantly, the changes in the intestinal flora, the metabolites and cytokines were investigated. In the stapled immobilization mice model, ATG-F4 treated group had significantly increased muscle mass, myofiber size, running time to be exhausted and grip strength. The cytokine levels in serum and muscle tissues were reduced by ATG-F4 treatment. Furthermore, the phosphorylation of proteins involved in muscle synthesis such as mTOR, p70S6K, rpS6 and 4E-BP1 increased and MuRF1 related to muscle atrophy factor reduced in the TA and GA muscles. ATG-F4 treatment changed the ratio of main intestinal microflora by increasing the family Muribaculaceae (phylum Bacteroidetes) and decreasing the family Lachnospiraceae (phylum Firmicutes) and Lactobacillaceae (phylum Firmicutes). Also, the level of short chain fatty acids (SCFAs) including butyric acid and acetic acid in the serum of ATG-F4 group were increased. These results suggest that L. reuteri ATG-F4 can inhibit muscle atrophy and it is associated with the microbiota and its metabolites with the anti-inflammation effect. ATG-F4 may be a potential prophylactic or therapeutic composition for muscle atrophy.

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“…Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 26 . Forkhead Box O1 27 . Forkhead Box O3…”

Section: Compliance With Ethical Guidelinesmentioning

confidence: 99%

Effect of Reduced Physical Activity on Murf-1 and Atrogin-1 Gene Expression in Soleus Muscle of Wistar Rats Following Endurance, Resistance and Combined Training

Madahi

Gharakhanlou²,

Kazemi³

et al. 2022

SJRM

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Background and Aims Understanding the involved mechanisms in muscle atrophy can help develop new treatment methods for atrophic conditions. Skeletal muscle atrophy via biochemical and transcriptional pathways can increase the expression of some muscle atrophy related genes. The present study aims to assess the effect of reduced physical activity by the spinal nerve ligation (SNL) after a period of resistance, endurance and combined exercises on the expression of MuRF-1 and Atrogin-1 genes in male rats. Methods In this experimental study, 30 male Wistar rats were randomly divided into four groups: Control+SNL, combined exercise+SNL, endurance exercise+SNL, and resistance training+SNL. The exercises were performed for six weeks. After this period, the protocol of reduced physical activity by the SNL was implemented for four weeks. At the end, soleus muscle was isolated and the expression levels of MuRF-1 and Atrogin-1 genes were measured by real-time polymerase chain reaction technique. To examine the gene expression differences between the groups, analysis of variance and Tukey’s post hoc test were used. The significant level was set at 0.05. Results The results showed that the expression of MuRF-1 gene in the combined exercise+SNL and resistance training+SNL groups was significantly lower than in the control group (P<0.05). The Atrogin-1 gene expression was significantly reduced only in the combined exercise+SNL group compared to the control group (P<0.05). Conclusion It seems that the rats with resistance training are more resistant to SNL-induced atrophy than the group with endurance exercise.

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Concurrent exercise training and Murf-l and Atrogin-1 gene expression in the vastus lateralis muscle of male Wistar rats

Cited by 5 publications

References 26 publications

Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice

Changes Induced by Aging and Long-Term Exercise and/or DHA Supplementation in Muscle of Obese Female Mice

Anti-muscle Atrophy Effects ofLactobacillus reuteriATG-F4 and Its Impact on Intestinal Microbiota and Metabolites: Implications for Prophylaxis and Therapy

Effect of Reduced Physical Activity on Murf-1 and Atrogin-1 Gene Expression in Soleus Muscle of Wistar Rats Following Endurance, Resistance and Combined Training

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